Sema6c (NM_011351) Mouse Tagged ORF Clone

CAT#: MG224118

Sema6c (tGFP-tagged) - Mouse sema domain transmembrane domain (TM) and cytoplasmic domain (semaphorin) 6C (Sema6c), (10ug)

Product Images

Circular map for MG224118

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sema6c
• Synonyms: mKIAA1869; Semay
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224118 representing NM_011351
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGTGCCCCCCACTCCATGCCCCTGCTGCTCCTGCTGCTGTTGCTGTCATCGCTCCCCCAAGCCC
  AGGCTGCCTTTCCCCAGGACCCCACCCCTCTGTTGACCTCTGACCTTCAAGGTGCCTCTCCATCATCCTG
  GTTCCGGGGCCTGGAGGACGATGCTGTGGCTGCGGAACTTGGGCTGGACTTTCAGAGATTCCTGACCTTG
  AACCGGACCTTGCTTGTGGCTGCCCGGGATCACGTTTTCTCCTTCGATCTTCAAGCCCAAGAAGAAGGGG
  AGGGGCTGGTGCCCAACAAGTTTCTGACATGGAGGAGTCAAGATATGGAGAACTGTGCAGTCCGGGGAAA
  GCTGACGGACGAATGCTACAACTACATCCGTGTTCTTGTTCCCTGGAACTCGCAGACACTTCTTGCCTGT
  GGAACAAATTCCTTCAGCCCTATGTGCCGCAGCTATGGGATAACATCTCTGCAACAGGAGGGTGAGGAGC
  TGAGTGGGCAGGCTCGATGCCCCTTTGATGCCACCCAGTCCACTGTGGCCATCTTTGCAGAGGGCAGTTT
  GTACTCAGCCACAGCAGCAGATTTCCAGGCCAGTGATGCTGTAGTTTACAGAAGTCTTGGACCTCAGCCT
  CCACTCCGTTCTGCAAAGTATGACTCCAAGTGGCTTCGAGAGCCACACTTTGTCTATGCGTTGGAGCATG
  GAGAGCACGTCTACTTCTTCTTCCGAGAAGTCTCTGTGGAGGATGCCCGCTTAGGGAGGGTGCAGTTTTC
  CAGGGTAGCCCGGGTATGTAAACGTGACATGGGTGGCTCACCTCGGGCCTTGGATCGCCACTGGACATCC
  TTCCTTAAGCTGAGGCTCAACTGCTCTGTCCCTGGGGACTCTACCTTCTACTTTGACGTCTTACAGTCCT
  TAACTGGGCCTGTGAACCTGCATGGCCGCTCCGCTCTCTTTGGGGTCTTCACTACTCAGACCAATAGCAT
  TCCTGGGTCTGCAGTCTGCGCCTTCTACCTAGATGATATTGAACGTGGCTTTGAGGGCAAGTTCAAGGAG
  CAGAGGAGTCTGGATGGGGCCTGGACTCCTGTGTCTGAGGACAAGGTCCCCTCACCCAGGCCAGGGTCCT
  GTGCAGGTGTGGGTGCAGCTGCCTCGTTCTCCTCCTCTCAAGACCTACCTGATGATGTCCTGCTCTTCAT
  CAAGGCACACCCGCTGCTGGATCCTGCTGTGCCACCCGCCACCCATCAACCGCTCCTCACTCTGACTAGC
  AGGGCCCTGCTGACCCAGGTAGCTGTGGACGGAATGGCTGGTCCCCACAGAAATACTACTGTCCTGTTTC
  TTGGCTCCAACGATGGGACAGTGCTGAAGGTGCTACCTCCAGGGGGTCAGTCTTTGGGCTCTGAGCCTAT
  CGTCTTGGAAGAGATTGATGCCTACAGCCATGCCCGGTGCAGTGGGAAGCGGTCACCCCGAGCTGCGCGG
  CGGATCATAGGGCTGGAGCTGGACACTGAGGGTCACAGGCTTTTTGTGGCCTTTCCTGGATGTATCGTCT
  ACCTCTCTCTCAGCCGGTGTGCCCGGCATGGAGCATGTCAGAGGAGCTGCCTGGCTTCTCTGGACCCATA
  CTGTGGATGGCATCGATCCAGAGGCTGCATGAGTATCAGGGGACCTGGTGGGACTGATGTCGATCTGACT
  GGGAACCAGGAATCCACAGAGCACGGTGACTGCCAAGATGGAGCGACCGGGAGTCAGTCTGGCCCTGGAG
  ATTCTGCTTATGGCGTGCGCAGGGACCTTTCCCCAGCCTCAGCCTCCCGATCCATTCCCATCCCACTCCT
  CCTGGCCTGTGTGGCCGCGGCCTTCGCTTTGGGCGCCTCAGTCTCCGGTCTCTTGGTGTCCTGTGCTTGT
  CGTCGCGCGAACCGCCGTCGGAGCAAGGACATCGAGACCCCGGGGCTGCCGCGCCCCCTCTCCCTTCGCA
  GCCTGGCCCGGCTGCACGGTGGCGGTCCTGAGCCCCCGCCTCCGCCCAAGGATGGAGATGCAGCGCAAAC
  GCCCCAGCTCTACACTACCTTCCTGCCTCCGCCCGACGGCGGATCCCCACCGGAGCTGGCCTGCCTACCC
  ACGCCGGAGACGACGCCCGAGCTGCCCGTGAAGCACCTCCGTGCCTCCGGGGGCCCCTGGGAATGGAACC
  AGAATGGGAACAACGCCTCGGAGGGCCCAGGCCGCCCACCACGGGGCTGCAGCGGGGCGGGCGGGCCCGC
  ACCGCGAGTGCTGGTGAGGCCACCGCCCCCTGGCTGCCCCGGGCAGGCGGTAGAGGTGACCACGCTGGAG
  GAACTGCTGCGCTACCTGCACGGCCCTCAGCCGCCCAGGAAGGGTAGCGAACCTCTCGCCTCCGCCCCGT
  TCACCTCCCGGCCGCCCGCCTCGGAGCCCGGCGCCTCGCTGTTCGTGGACTCCAGCCCGATGCCGCGGGA
  TGGCGTTCCGCCGCTCAGGCTCGACGTGCCACCCGAAGGCAAGCGCGCTGCCCCGAGCGGGCGGCCTGCT
  CTCTCGGCCCCAGCCCCGCGCCTGGGTGTCGGCGGCAGCCGCCGATTGCCCTTTCCCACACACCGGGCGC
  CCCCGGGCCTGCTCACGCGAGTGCCCTCGGGAGGCCCGGCCAGGTACTCCGGGGGGCCCGGGAGGCACCT
  CCTGTACCTGGGCCGGCCCGAAGGCCATCGCGGCCGCTCCCTGAAGAGGGTGGACGTGAAGTCTCCGCTG
  TCGCCCAAGCCGCCCCTCGCCTCCCCGCCGCAGCCCGCCCCGCACGGTGGTCATTTCAACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224118 representing NM_011351
  Red=Cloning site Green=Tags(s)
  
  MPRAPHSMPLLLLLLLLSSLPQAQAAFPQDPTPLLTSDLQGASPSSWFRGLEDDAVAAELGLDFQRFLTL
  NRTLLVAARDHVFSFDLQAQEEGEGLVPNKFLTWRSQDMENCAVRGKLTDECYNYIRVLVPWNSQTLLAC
  GTNSFSPMCRSYGITSLQQEGEELSGQARCPFDATQSTVAIFAEGSLYSATAADFQASDAVVYRSLGPQP
  PLRSAKYDSKWLREPHFVYALEHGEHVYFFFREVSVEDARLGRVQFSRVARVCKRDMGGSPRALDRHWTS
  FLKLRLNCSVPGDSTFYFDVLQSLTGPVNLHGRSALFGVFTTQTNSIPGSAVCAFYLDDIERGFEGKFKE
  QRSLDGAWTPVSEDKVPSPRPGSCAGVGAAASFSSSQDLPDDVLLFIKAHPLLDPAVPPATHQPLLTLTS
  RALLTQVAVDGMAGPHRNTTVLFLGSNDGTVLKVLPPGGQSLGSEPIVLEEIDAYSHARCSGKRSPRAAR
  RIIGLELDTEGHRLFVAFPGCIVYLSLSRCARHGACQRSCLASLDPYCGWHRSRGCMSIRGPGGTDVDLT
  GNQESTEHGDCQDGATGSQSGPGDSAYGVRRDLSPASASRSIPIPLLLACVAAAFALGASVSGLLVSCAC
  RRANRRRSKDIETPGLPRPLSLRSLARLHGGGPEPPPPPKDGDAAQTPQLYTTFLPPPDGGSPPELACLP
  TPETTPELPVKHLRASGGPWEWNQNGNNASEGPGRPPRGCSGAGGPAPRVLVRPPPPGCPGQAVEVTTLE
  ELLRYLHGPQPPRKGSEPLASAPFTSRPPASEPGASLFVDSSPMPRDGVPPLRLDVPPEGKRAAPSGRPA
  LSAPAPRLGVGGSRRLPFPTHRAPPGLLTRVPSGGPARYSGGPGRHLLYLGRPEGHRGRSLKRVDVKSPL
  SPKPPLASPPQPAPHGGHFNF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011351
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011351.2, NP_035481.1
• RefSeq Size: 2843 bp
• RefSeq ORF: 2796 bp
• Locus ID: 20360
• UniProt ID: Q9WTM3
• Cytogenetics: 3 F2.1
• Gene Summary: May be a stop signal for the dorsal root ganglion neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections (By similarity).[UniProtKB/Swiss-Prot Function]