Tcp11 (NM_013687) Mouse Tagged ORF Clone

CAT#: MG223625

Tcp11 (tGFP-tagged) - Mouse t-complex protein 11 (Tcp11) transcript variant 1, (10ug)

Product Images

Circular map for MG223625

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tcp11
• Synonyms: D17Ken1; Tcp-11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223625 representing NM_013687
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGACGTGAAAGAGAGGGCGGCCCGGAAGGAGCCCGGCGCGGCAGAGAGCGCCTCCCGTGAATCCC
  GCGGGGGAAACACCCGGGAGAGCGCGAGCAGCGCCCAGGGGCACCGATCGTGTAGGTTCAACCGTCGCCC
  GAGCACGGCCGCCCTCACCCCAGGGTCCGCGCAGGGGCGCGGGGTAAAGACGGCGCCACGAGGTCCCGTG
  GGCCACGGAGGACTGCGGACTGGCCCGACCTCCCGGTGTCCCCAGCCCTCCGCCCGAGCGAAGCTCCCCT
  CGGTCACGCGTGGGGCACCGCTTCCGCCATCCCCGGGAAAGGGCCACCTCGGCGGGACCCCGAGCTCGCA
  CAGGCTAGGTATGACGGAGAGAGTCCATGACGCTTCCAAGCTTGATTGTCAGTTGGAAGAAAGGAGTTTA
  TCTTCAAGCAGCCTGAAAGGCAAGGTCAAGGACACCATGCCCAGCGACTTCTGGGAGCATCTGAATGAGC
  AGCTGTCAGCCGTGCCCCCCGACTTCAGCTGTGCTCTTGAGCTTCTGAAGGAGATCAAAGAGATCCTGTT
  GTCCCTGCTGCTGCCACGGCAGAGCCGCCTGAAGAATGAGATCGAGGAAGCTCTGGACATGGAGTTCCTC
  CAGCAGCAAGCCGACCGCGGAGACCTGAATGTCTCCTACCTCTCCAAGTATATCCTCAACATGATGGTCT
  TGTTGTGTGCGCCAATCCGAGACGAGGCTGTGCAGAGACTTGAGAACATTTCAGATCCGGTCCGGCTGCT
  GAGGGGGATCTTCCAGGTCTTGGGACAGATGAAAATGGACATGGTGAACTACACCATCCAGAGCCTCCAG
  CCCCAGCTTCAGGAACACTCCGTCCAGTTTGAGCGGGCTCAGTTCCAGGAGCGCCTCAACAAAGAGCCCA
  GACTCCTCAACCACACCACGAAGTGGCTCACCCAGGCGGCCACACAGCTCATTGCCCCCTCTGCCAGTTC
  CTCTGACTTGCAGGACTGCTCCAGCTCGGCCGGCCCATCTCCCAGTGATGTCGCCGTCCCAGAGCCACTC
  AGCCCCGCGATGGTGCTGTCTCAAGGATTCCTGAACCTTCTCACCTGGGACCCTGAGAATGAAGAGTTTC
  CTGAAACCCTAGTGGCAGACAGACCCCGGCTGCAGGAGCTGGAGTCGCAGCAGAGTCAGCTAACCATCCT
  TGCCTCTGTCTTGCTGGTGGCCAGTAGCTTCTCTGACAGTGGTCTGTTTAGCTCACCCCAGTTTGTAGAC
  AAGCTGAAACAAATCACGAAGTCCCTGGTGGAGGATTTCAACTCCAGGCCCGAGGAAGTGATGCAGTCAG
  TGAGTGAGCAGGTGGTGGAGGAAGTCCACCAGGGTCTCGAGAGCATGGGGTTGGCCGCTCTGAGCAGCGA
  GAATACGGCGTCCCTGGTGGGCCAGCTCCAGAACATTGCCAAGAAGGAAAACTGCGTCCGCAGCGTCATT
  GACCAGCGTATACACTTGTTCCTCAAGTGCTGCTTTGTCCTGGGTGTGCAGCGATCCCTCTTGGACCTTC
  CCGGGGGCCTCACTCTGATCGAAGCTGAGCTGGCGGAACTGGGCCAGAAGTTTGTCAGCCTGACCCATCA
  CAACCAGCAGGTGTTTGCCCCGTATTACACCGAGATCTTAAAAACCCTCATCTCCCCAGCCCAGACCCTG
  GCCACCAAAGGTGGGTCTCTC
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223625 representing NM_013687
  Red=Cloning site Green=Tags(s)
  
  MPDVKERAARKEPGAAESASRESRGGNTRESASSAQGHRSCRFNRRPSTAALTPGSAQGRGVKTAPRGPV
  GHGGLRTGPTSRCPQPSARAKLPSVTRGAPLPPSPGKGHLGGTPSSHRLGMTERVHDASKLDCQLEERSL
  SSSSLKGKVKDTMPSDFWEHLNEQLSAVPPDFSCALELLKEIKEILLSLLLPRQSRLKNEIEEALDMEFL
  QQQADRGDLNVSYLSKYILNMMVLLCAPIRDEAVQRLENISDPVRLLRGIFQVLGQMKMDMVNYTIQSLQ
  PQLQEHSVQFERAQFQERLNKEPRLLNHTTKWLTQAATQLIAPSASSSDLQDCSSSAGPSPSDVAVPEPL
  SPAMVLSQGFLNLLTWDPENEEFPETLVADRPRLQELESQQSQLTILASVLLVASSFSDSGLFSSPQFVD
  KLKQITKSLVEDFNSRPEEVMQSVSEQVVEEVHQGLESMGLAALSSENTASLVGQLQNIAKKENCVRSVI
  DQRIHLFLKCCFVLGVQRSLLDLPGGLTLIEAELAELGQKFVSLTHHNQQVFAPYYTEILKTLISPAQTL
  ATKGGSL
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013687
• ORF Size: 1701 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013687.3, NP_038715.2
• RefSeq Size: 2001 bp
• RefSeq ORF: 1704 bp
• Locus ID: 21463
• Cytogenetics: 17 14.62 cM