Sts (NM_009293) Mouse Tagged ORF Clone
CAT#: MG223101
- TrueORF®
Sts (tGFP-tagged) - Mouse steroid sulfatase (Sts), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_009293" in other vectors (2)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Sts |
Synonyms | ArsC |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223101 representing NM_009293, codon optimized.
Due to the complexity of NM_009293, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGGCCCAGACCCTTGCTGCTGGCGGTGATGGCCGCTACTTTGGCCGATATAATCCTGGCTGCCG ATCCCGCGCCCGCCGGACCCGCTCCGAGGCCTCCCAACTTCCTTTTGATCATGGCAGATGATCTTGGAAT CGGAGATCTTGGCTGCTACGGAAACAAAACGTTGCGGACTCCACACCTGGACCGCCTGGCTAGGGAGGGA GTGAAACTCACTCAACACCTGGCTGCTGCTCCCCTTTGTACTCCTTCCCGGGCCGCCTTCCTGACCGGCC GCTACCCACCACGGTCAGGCATGGCTGCCCACGGCCGGGTCGGTGTATACTTGTTTACCGCGTCTAGCGG GGGGCTCCCTCCATCCGAAGTCACCATGGCACGCCTGCTGAAGGGACGGGGCTATGCTACCGCTCTTATC GGCAAATGGCACCTGGGGCTGTCCTGTAGGGGCGCCACCGACTTTTGTCACCATCCGCTGAGGCATGGCT TTGATAGGTTCCTCGGAGTGCCAACTACCAACTTGCGCGACTGCAGACCTGGCGCCGGGACCGTATTTGG CCCTGCTCTCCGGGTGTTTGCGGCAGGCCCACTGGCTGCCCTCGGGGCATCACTTGCAGCTATGGCCGCT GCCAGATGGGCAGGGCTTGCTAGAGTGCCAGGGTGGGCTTTGGCTGGTACTGCCGCCGCAATGCTGGCCG TCGGGGGCCCTCGGTCCGCTAGTTGCTTGGGGTTTAGGCCAGCAAACTGCTTCCTCATGGATGATCTGGC TGTTGCACAAAGGCCAACCGACTATGGCGGCCTCACCAGACGGCTGGCTGACGAGGCCGCCCTGTTTCTT CGGCGCAATCGCGCAAGACCGTTCCTCCTTTTCCTGAGCTTCCTGCACGTTCACACAGCACACTTCGCCG ACCCTGGGTTCGCAGGGCGCTCTCTGCACGGGGCCTACGGGGATTCTGTAGAAGAGATGGACTGGGGTGT GGGTAGGGTCCTCGCTGCCCTGGATGAGCTGGGACTGGCTAGAGAAACCCTGGTTTACTTTACTTCAGAC CACGGGGCACACGTGGAGGAGCTGGGGCCCAGAGGAGAGCGAATGGGAGGCTCCAACGGTGTCTTCCGAG GCGGCAAGGGAAATAACTGGGAAGGCGGCGTCCGCGTGCCTTGCCTGGTCAGATGGCCTCGCGAACTCAG TCCTGGACGGGTCGTTGCCGAACCCACCAGCTTGATGGATGTCTTCCCCACAGTAGCCAGACTCGCCGGA GCCGAACTGCCAGGCGACAGGGTTATCGATGGTCGCGACCTGATGCCTTTGCTTCGCGGCGATGCGCAGA GGAGCGAGCATGAATTCCTTTTTCACTACTGTAACGCCTACTTGCAGGCAGTCCGCTGGCACAATGGAAG CGCTGTGTGGAAGGCCTTTTACTTCACCCCTAATTTCGCCCCCGCTGGCGCCAACGGGTGTTTCAGCACT CACGTCTGCCTTTGCGCCGGTCCTGCCCACGTTACTGCACACGACCCTCCCCTGCTGTTCGACCTGACTC GGGATCCTGGAGAAAGGCGACCTCTTACGCCAGAAGCCGAGCCGCGGCACCGAGAGGTCCTCGATGCAAT CGACGCAGCAGCCAGAGCTCACCGAGCACGCCTGCGGCCAGCCCCCGACCAGCTGGCTCCTCGCCACCTT ATGTGGAAACCATGGCTGCAGCTGTGGGGTGGAGGGGGTGCTGGGGGTGGCGCCGGTGCACAGGACGATT CCGGGCACGCCCACGGCGACGGCAGCCATGCCCATGACGACCCTGGTCACGCCCAAGACCGGGGTGACGA TGATGCACATTATGGTGGCCACGCGACCACTCGCACACAGGCCACCCCACGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223101 representing NM_009293
Red=Cloning site Green=Tags(s) MPRPRPLLLAVMAATLADIILAADPAPAGPAPRPPNFLLIMADDLGIGDLGCYGNKTLRTPHLDRLAREG VKLTQHLAAAPLCTPSRAAFLTGRYPPRSGMAAHGRVGVYLFTASSGGLPPSEVTMARLLKGRGYATALI GKWHLGLSCRGATDFCHHPLRHGFDRFLGVPTTNLRDCRPGAGTVFGPALRVFAAGPLAALGASLAAMAA ARWAGLARVPGWALAGTAAAMLAVGGPRSASCLGFRPANCFLMDDLAVAQRPTDYGGLTRRLADEAALFL RRNRARPFLLFLSFLHVHTAHFADPGFAGRSLHGAYGDSVEEMDWGVGRVLAALDELGLARETLVYFTSD HGAHVEELGPRGERMGGSNGVFRGGKGNNWEGGVRVPCLVRWPRELSPGRVVAEPTSLMDVFPTVARLAG AELPGDRVIDGRDLMPLLRGDAQRSEHEFLFHYCNAYLQAVRWHNGSAVWKAFYFTPNFAPAGANGCFST HVCLCAGPAHVTAHDPPLLFDLTRDPGERRPLTPEAEPRHREVLDAIDAAARAHRARLRPAPDQLAPRHL MWKPWLQLWGGGGAGGGAGAQDDSGHAHGDGSHAHDDPGHAQDRGDDDAHYGGHATTRTQATPR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009293 |
ORF Size | 1872 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009293.1, NP_033319.1 |
RefSeq Size | 2283 bp |
RefSeq ORF | 1875 bp |
Locus ID | 20905 |
UniProt ID | P50427 |
Cytogenetics | XY 75.0 cM |
Gene Summary | Catalyzes the conversion of sulfated steroid precursors, such as dehydroepiandrosterone sulfate (DHEA-S) and estrone sulfate to the free steroid.[UniProtKB/Swiss-Prot Function] |
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FAQs |
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