Nudt12 (NM_026497) Mouse Tagged ORF Clone

CAT#: MG222776

Nudt12 (tGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 12 (Nudt12), (10ug)

Product Images

Circular map for MG222776

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nudt12
• Synonyms: 0610016O18Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222776 representing NM_026497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCTGTTAAAAGAAATCCCAAAAAGGAGATGATTTCTGAACTCCACAGTTCAGCCGCAGAGGGAA
  ATGTCGCAAAGTTAGCAGGAATACTCAGCCATTCTCCATCTCTTCTCAATGAGACTTCTGAAAATGGATG
  GACTGCTTTAATGTACGCCGCCAGGAACGGGCATCCCGATGTGGTCCAGTTTCTGCTTGAGAAAGGATGT
  GACAGATCACTTGTCAATAAAGCGAGGCAAACCGCCCTGGATATTGCTGCATTTTGGGGTTATAGGCATA
  TAGCTAACTTGTTAGCAAATGCAAAAGGTGGGAAGAAGCCCTGGTTCCTAACCAATGAAGTAGACGAGTG
  TGAGAATTATTTTAGCAGAACACTACTGGACCGGAGGAGTGACAAAAGAAATAATTCTGACTGGCTGCAA
  GCTAAAGAGAGCCACCCCACCACAGTTTATCTCCTTTTCTCAGACTTGAACCCCCTGGTTACCCTAGGTG
  GTAATAAAGAAAGCTCGCAGCAGCCGGAAGTCCGGCTTTGCCAGCTGAACTACCCGGATGTAAAGGGTTA
  CTTGGCTCAGCCTGAGAAGATCACCTTGGTGTTCCTTGGAGTCGAGCTTGAGATGAGGAAAGGCTCACCC
  GCCCAGGCGGGAGGAGTCCCAGAGGAAGAGGAGGACGGTTTGGTTGCTTGGTTTGCCCTCGGTATTGAAC
  CCGGTGCTGCTGAGGAGTTTAAGCAAAGACATGAAAATTGTTATTTTCTCCACCCGCCAATGCCAGCTCT
  TCTGCAGTTGAAAGAAAAGGAGGCTGGGGTGGTAGCTCAAGCAAGATCAGTGCTTGCCTGGCATAGTCGA
  TACAAGTTCTGCCCAACCTGTGGCAGTGCGACTAAAATTGAGGAAGGAGGCTACAAAAGAGTGTGTGTAC
  GAGAGACTTGCCCTAGTCTCCAAGGCGTCCACAACACATCTTACCCGAGAGTTGATCCAGTCGTAATTAT
  GCAGGTCATCCATCCAGATGGAACCAAATGTCTTCTAGGCAGGCAAAAGCGATTCCCCCCGGGCATGTTC
  ACATGTCTTGCTGGATTTATAGAGCCCGGGGAGACAATAGAAGATGCTGTGCGGAGAGAGGTGGAAGAGG
  AAAGTGGAGTCAAAGTTGGCCATGTTCAGTATGTCTCTTGTCAACCATGGCCAATGCCCTCCTCGTTAAT
  GATTGGCTGTTTAGCTGTGGCAGTGTCTACAGAAATTAAAGTTGACAAGAATGAAATAGAGGATGCCCGA
  TGGTTCACTAGAGAACAGGTTGTGGACGTTCTTACCAAAGGGAAGCAGCAAGCATTCTTTGTGCCACCAA
  GCCGAGCTATCGCACATCAGTTAATCAAACACTGGGTGGGAATGAACCCCAATCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222776 representing NM_026497
  Red=Cloning site Green=Tags(s)
  
  MSSVKRNPKKEMISELHSSAAEGNVAKLAGILSHSPSLLNETSENGWTALMYAARNGHPDVVQFLLEKGC
  DRSLVNKARQTALDIAAFWGYRHIANLLANAKGGKKPWFLTNEVDECENYFSRTLLDRRSDKRNNSDWLQ
  AKESHPTTVYLLFSDLNPLVTLGGNKESSQQPEVRLCQLNYPDVKGYLAQPEKITLVFLGVELEMRKGSP
  AQAGGVPEEEEDGLVAWFALGIEPGAAEEFKQRHENCYFLHPPMPALLQLKEKEAGVVAQARSVLAWHSR
  YKFCPTCGSATKIEEGGYKRVCVRETCPSLQGVHNTSYPRVDPVVIMQVIHPDGTKCLLGRQKRFPPGMF
  TCLAGFIEPGETIEDAVRREVEEESGVKVGHVQYVSCQPWPMPSSLMIGCLAVAVSTEIKVDKNEIEDAR
  WFTREQVVDVLTKGKQQAFFVPPSRAIAHQLIKHWVGMNPNL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026497
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026497.3
• RefSeq Size: 2280 bp
• RefSeq ORF: 1389 bp
• Locus ID: 67993
• UniProt ID: Q9DCN1
• Cytogenetics: 17 D
• Gene Summary: Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)(+), ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle (By similarity).[UniProtKB/Swiss-Prot Function]