Setdb1 (NM_001163641) Mouse Tagged ORF Clone
CAT#: MG222601
- TrueORF®
Setdb1 (tGFP-tagged) - Mouse SET domain bifurcated 1 (Setdb1) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001163641" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Setdb1 |
Synonyms | AU022152; ESET; KMT1E; mKIAA0067 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222601 representing NM_001163641
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCCCTCCCTGGGTGCATGAGTTTGGCTGCAGCGCCAGCTGCAGCTGACTCTGCAGAGATTGCTG AGCTGCAGCAGGCGGTGGTTGAAGAGCTGGGTATCTCTATGGAGGAACTTCGTCAGTACATTGATGAGGA ACTGGAAAAGATGGACTGCATACAGCAGCGCAAGAAGCAGCTCGCAGAGCTGGAGACGTGGGTACTACAG AAAGAGTCTGAAGTGGCTTATGTTGATCGGCTGTTTGATGATGCATCCAGGGAAGTGACTAACTGTGAGT CTTTGGTGAAGGATTTCTACTCTAAGCTGGGACTACAGTATCATGACAGTAGCTCTGAGGATGAAGCTTC CCGGCCCACAGAGATCATTGAGATTCCTGATGAAGATGATGATGTCCTCAGTATTGATTCAGGTGATGCT GGGAGCAGAACTCCAAAAGACCAGAAGCTTCGTGAAGCTATGGCTGCCTTAAGAAAATCAGCTCAAGATG TCCAGAAGTTCATGGATGCTGTCAACAAGAAAAGCAGTTCTCAAGATCTACATAAAGGAACCTTGGGTCA GGTGTCTGGAGAACTGAGCAAAGATGGGGACCTGATAGTCAGCATGCGGATTCTGGGCAAGAAGAGGACT AAGACATGGCACAAAGGCACCCTTATTGCCATCCAGACTGTTGGGCTAGGAAAAAAATACAAAGTGAAAT TTGACAACAAAGGAAAGAGTCTGCTATCTGGGAACCATATTGCCTATGATTACCACCCTCCCGCTGACAA GCTGTTTGTGGGCAGTCGAGTGGTGGCCAAGTACAAAGATGGAAATCAGGTCTGGCTTTATGCTGGCATT GTAGCTGAGACCCCTAACGTCAAGAACAAGCTCAGATTTTTAATTTTTTTTGATGATGGCTATGCTTCCT ATGTCACTCAGTCAGAGCTTTATCCCATTTGCCGACCACTAAAAAAGACTTGGGAGGACATAGAAGATAG CTCCTGCCGAGACTTCATAGAGGAATATATCACTGCCTATCCAAACCGCCCAATGGTACTTCTCAAGAGT GGGCAGCTTATCAAGACTGAGTGGGAAGGCACATGGTGGAAGTCTCGAGTTGAAGAGGTGGATGGCAGCC TAGTCAGGATCCTCTTTCTGGATGACAAAAGATGTGAGTGGATATATCGAGGCTCTACACGCCTGGAACC TATGTTTAGTATGAAGACATCCTCAGCCTCTGCAATGGAGAAGAAGCAAGGGGGGCAACTCAGAACCCGT CCTAATATGGGTGCTGTGAGGAGCAAAGGTCCTGTTGTTCAGTATACACAGGATCTAACTGGTACTGGAA TCCAGTTTAAGCCCATGGAGCCCCTACAGCCTATAGCTCCACCGGCCCCACTTCCTATACCTCCTCTTTC CCCCCAAGCAGCTGACACTGAAAGCTTAGAAAGCCAACTTGCACAATCACGGAAACAAGTAGCCAAGAAG AGCACATCATTCCGACCAGGATCTGTGGGCTCCGGCCATTCCTCCCCTACTTCATCCACACTCAGTGAAA ATGTGTCTGCTGGGAAACTTGGGATAAACCAGACATATCGGTCACCTTTGGCCTCAGTAACATCTACCCC AGCATCTGCAGCCCCTCCAGTCCCTCCAGTCCCACCAGGGCCTCCAACCCCTCCAGGGCCTCCAGCTCCT CCAGGGCCTCTAGCTCCTCCAGCCTTCCATGGCATGTTAGAGCGGGCACCAGCTGAGCCCTCCTACCGAG CCCCCATGGAGAAGCTTTTCTATTTACCTCATGTCTGCAGTTACACTTGTTTGTCCCGGATCAGACCCAT GAGAAACGAACAGTATCGGGGCAAGAACCCTCTATTAGTTCCACTTCTGTATGACTTCCGGAGGATGACA GCACGGCGCAGAGTTAACCGCAAAATGGGCTTTCATGTAATCTATAAGACACCCTGTGGTCTCTGCCTTC GGACGATGCAGGAGATAGAGCGCTACCTTTTTGAGACTGGCTGTGACTTTCTGTTCCTGGAGATGTTCTG TTTGGATCCATATGTTCTTGTTGACAGAAAGTTTCAACCCTTTAAGCCTTTTTACTATATTTTGGACATC ACCTATGGCAAGGAAGATGTTCCCCTGTCCTGTGTTAATGAGATTGACACAACTCCCCCACCCCAGGTGG CCTACAGCAAGGAACGCATTCCTGGCAAGGGTGTTTTCATTAACACAGGCCCTGAATTTCTGGTTGGCTG TGACTGCAAGGATGGGTGTCGGGATAAATCCAAATGTGCCTGCCACCAGCTAACTATCCAGGCCACAGCC TGTACCCCAGGGGGCCAAGTCAACCCTAACTCTGGCTACCAGTATAAAAGACTAGAAGAGTGTCTGCCCA CAGGGGTTTATGAGTGTAACAAACGCTGCAATTGTGACCCAAACATGTGCACAAATCGGTTGGTGCAGCA TGGTCTGCAGGTTCGACTACAGCTGTTTAAGACACAGAACAAGGGCTGGGGTATCCGCTGCTTGGATGAT ATTGCCAAAGGCTCTTTTGTCTGCATTTATGCAGGCAAAATCCTGACAGATGACTTTGCAGACAAAGAAG GCCTGGAGATGGGTGATGAGTACTTTGCAAATCTGGACCACATTGAAAGTGTGGAGAACTTCAAGGAAGG ATATGAGAGTGATGTCCCCACTTCCTCTGACAGCAGTGGGGTAGATATGAAGGACCAGGAAGATGGCAAC AGCGGTTCAGAGGACCCTGAAGAATCCAATGATGACAGCTCTGATGATAACTTCTGTAAGGATGAGGACT TCAGCACCAGTTCAGTGTGGCGTAGCTATGCTACCCGGAGGCAGACTCGGGGTCAAAAGGAGAATGAATT GTCTGAGATGACTTCCAAGGACTCCCGCCCCCCAGACCTCGGGCCTCCACATGTTCCTATCCCTTCCTCA GTATCTGTAGGGGGCTGCAATCCACCTTCCTCTGAAGAGACACCCAAGAACAAGGTGGCCTCGTGGTTGA GTTGCAATAGTGTCAGTGAAGGTGGATTTGCTGACTCTGACAGCCGTTCTTCCTTCAAGACTAGTGAAGG TGGAGATGGCCGTGCTGGGGGAGGCCGGGGAGAGGCTGAAAGGGCCTCTACCTCAGGATTGAGCTTCAAG GATGAAGGAGACAATAAGCAGCCTAAAAAAGAGGACCCTGAGAACCGAAACAAGATGCCAGTAGTTACTG AAGGCTCTCAGAATCATGGACATAATCCTCCCATGAAGTCTGAAGGGCTTCGCCGATCAGCTAGTAAAAT GTCTGTGCTCCAGAGCCAGCGAGTTGTGACTTCTACTCAGTCAAACCCTGATGACATCCTGACACTGTCC AGCAGCACAGAGAGTGAGGGGGAAAGTGGAACCAGCCGAAAGCCCACTGCTGGTCACACTTCAGCCACAG CTGTTGATAGTGATGACATCCAGACCATCTCTTCTGGCTCTGACGGTGATGACTTTGAGGACAAGAAGAA CTTGTCAGGACCAACAAAGCGCCAGGTGGCAGTAAAATCAACCCGAGGCTTTGCTCTTAAATCAACCCAT GGTATTGCCATTAAATCAACCAACATGGCTTCCGTGGACAAGGGGGAGAGTGCACCAGTTCGTAAGAACA CACGCCAGTTCTATGATGGTGAAGAGTCTTGCTACATCATTGATGCCAAACTTGAAGGCAACCTAGGCCG CTACCTCAATCACAGTTGCAGCCCCAACCTGTTTGTCCAGAATGTGTTTGTGGATACCCATGATCTTCGC TTCCCTTGGGTGGCCTTCTTTGCCAGCAAGAGAATCCGGGCTGGAACAGAACTCACTTGGGACTACAACT ACGAAGTGGGCAGTGTGGAAGGCAAGGAGCTGCTGTGCTGCTGTGGGGCCATTGAATGCAGAGGGAGACT TCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222601 representing NM_001163641
Red=Cloning site Green=Tags(s) MSSLPGCMSLAAAPAAADSAEIAELQQAVVEELGISMEELRQYIDEELEKMDCIQQRKKQLAELETWVLQ KESEVAYVDRLFDDASREVTNCESLVKDFYSKLGLQYHDSSSEDEASRPTEIIEIPDEDDDVLSIDSGDA GSRTPKDQKLREAMAALRKSAQDVQKFMDAVNKKSSSQDLHKGTLGQVSGELSKDGDLIVSMRILGKKRT KTWHKGTLIAIQTVGLGKKYKVKFDNKGKSLLSGNHIAYDYHPPADKLFVGSRVVAKYKDGNQVWLYAGI VAETPNVKNKLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDSSCRDFIEEYITAYPNRPMVLLKS GQLIKTEWEGTWWKSRVEEVDGSLVRILFLDDKRCEWIYRGSTRLEPMFSMKTSSASAMEKKQGGQLRTR PNMGAVRSKGPVVQYTQDLTGTGIQFKPMEPLQPIAPPAPLPIPPLSPQAADTESLESQLAQSRKQVAKK STSFRPGSVGSGHSSPTSSTLSENVSAGKLGINQTYRSPLASVTSTPASAAPPVPPVPPGPPTPPGPPAP PGPLAPPAFHGMLERAPAEPSYRAPMEKLFYLPHVCSYTCLSRIRPMRNEQYRGKNPLLVPLLYDFRRMT ARRRVNRKMGFHVIYKTPCGLCLRTMQEIERYLFETGCDFLFLEMFCLDPYVLVDRKFQPFKPFYYILDI TYGKEDVPLSCVNEIDTTPPPQVAYSKERIPGKGVFINTGPEFLVGCDCKDGCRDKSKCACHQLTIQATA CTPGGQVNPNSGYQYKRLEECLPTGVYECNKRCNCDPNMCTNRLVQHGLQVRLQLFKTQNKGWGIRCLDD IAKGSFVCIYAGKILTDDFADKEGLEMGDEYFANLDHIESVENFKEGYESDVPTSSDSSGVDMKDQEDGN SGSEDPEESNDDSSDDNFCKDEDFSTSSVWRSYATRRQTRGQKENELSEMTSKDSRPPDLGPPHVPIPSS VSVGGCNPPSSEETPKNKVASWLSCNSVSEGGFADSDSRSSFKTSEGGDGRAGGGRGEAERASTSGLSFK DEGDNKQPKKEDPENRNKMPVVTEGSQNHGHNPPMKSEGLRRSASKMSVLQSQRVVTSTQSNPDDILTLS SSTESEGESGTSRKPTAGHTSATAVDSDDIQTISSGSDGDDFEDKKNLSGPTKRQVAVKSTRGFALKSTH GIAIKSTNMASVDKGESAPVRKNTRQFYDGEESCYIIDAKLEGNLGRYLNHSCSPNLFVQNVFVDTHDLR FPWVAFFASKRIRAGTELTWDYNYEVGSVEGKELLCCCGAIECRGRLL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163641 |
ORF Size | 3924 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163641.1, NP_001157113.1 |
RefSeq Size | 4682 bp |
RefSeq ORF | 3927 bp |
Locus ID | 84505 |
Cytogenetics | 3 F2.1 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224204 | Setdb1 (untagged) - Mouse SET domain, bifurcated 1 (Setdb1), transcript variant 1, (10ug) |
USD 1,253.00 |
|
MR222601 | Setdb1 (Myc-DDK-tagged) - Mouse SET domain, bifurcated 1 (Setdb1), transcript variant 1 |
USD 1,252.00 |
|
MR222601L3 | Lenti ORF clone of Setdb1 (Myc-DDK-tagged) - Mouse SET domain, bifurcated 1 (Setdb1), transcript variant 1 |
USD 1,552.00 |
|
MR222601L4 | Lenti ORF clone of Setdb1 (mGFP-tagged) - Mouse SET domain, bifurcated 1 (Setdb1), transcript variant 1 |
USD 1,552.00 |
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