Rnf103 (NM_009543) Mouse Tagged ORF Clone
CAT#: MG222470
- TrueORF®
Rnf103 (tGFP-tagged) - Mouse ring finger protein 103 (Rnf103), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_009543" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Rnf103 |
Synonyms | AW146237; AW212918; kf-1; Zfp10; Zfp103 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222470 representing NM_009543
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTGAAGCTGTTTTTCTTGCTCCTGTATTTCCTGGTCCTGTTCGTCCTGGCCAGGTTTTTTGAGG CCATTGTGTGGTACGAGACTGGCATCTTTGCTACTCAGCTGGTGGATCCGGTGGCATTGAGCTTCAAGAA GCTGAAGACCATTCTGGAGTGTCGAGGGCTGGGCTACTCCGGGCTGCCGGAGAAGAAAGATGTTCGGGAG CTGGTGGAGAAGTCAGGTGACTTGATGGAAGGTGAACTCTATTCTGCTCTTAAGGAAGAAGAGGCATCTG AGTCTGTTTCTAGTACCAATTTCAGTGGTGAAATGCATTTCTATGAGCTTGTGGAAGACACAAAAGATGG CATCTGGCTGGTTCAGGTCATAGCAAATGACAGAAGTCCTTTGGTGGGTAAAATCCACTGGGAGAAAATG GTGAAAAAAGTGTCAAGATTTGGAATACGGACAGGCACTTTCAACTGTTCCAGTGATCCCAGGTACTGCA GAAGGAGAGGCTGGGTGCGTTCCACACTCATCATGTCTGTGCCACAAACAAGTACATCTAAAGGGAAAGT CATGCTTAAAGAGTACAGTGGACGCAAGATTGAAGTAGAGCACATTTTTAAGTGGATAACTGCCCATGCA GCTTCTCGGATCAAAACTATATATAATGTGGAGCATTTGAAGGAAGAATGGAATAAAAGTGATCAATACT GGGTAAAAATATACCTATTTGCAAACCTTGACCAACCGCCAGCTTTCTTCTCTGCATTAAGTATAAAATT TACTGGAAGAGTTGAGTTTATTTTTGTTAATGTGGAAAATTGGAACAACAAGAGTTATATGACAGATATT GGTATTTATAACATGCCATCATACATACTTAGAACTCCTGAAGGAATTTATAGATACGGAAACCACACAG GTGAATTTATATCCCTTCAGGCCATGGATTCATTTCTGCGCTCATTACAACCTGAAGTAAATGATCTGTT TGTTTTGAGTTTGGTTCTAGTTAATCTTATGGCTTGGATGGACTTATTTATTACACAAGGAGCAACCATC AAGCGATTTGTGGTTCTCATAAGCACTTTAGGGACATACAATTCCCTATTAATTATTTCTTGGCTACCTG TGTTGGGCTTTCTACAGCTCCCTTACTTAGATAGCTTTTATGACTATAGTTTAAGATTGCTGCGGTATTC TAATACAACCACACTGGCTTCGTGGGTAAGGGCAGACTGGATGTTTTACACTTCACACCCAGCCCTGTTT CTCAGTACATACCTTGGACATGGTTTGCTAATTGATTACTTTGAGAAGAAGAGACGTCGCAGCAACAATG ATGAAGTTAATGCGAATAATTTAGAATGGTTATCAAGTCTGTGGGACTGGTACACCAGCTACCTCTTCCA CCCGATTGCTTCTTTTCAGAACTTTCCTGTAGACTCTGATTGGGACGAAGACCCTGACTTATTCTTGGAA CGGTTAGCTTTCCCTGACCTTTGGCTTCACCCTCTGATACCAACTGATTATATTAAAAACTTACCAATGT GGCGATTTAAATGTCTTGGGGTTCAGTCTGAAGAAGAAATGTCAGAGAGTTCTCAAGACACTGAAAATGA CTCGGATAGTGACAACATGGACACTTTTAGTAGTAGTAAGGATATATTTGAAGATAAACAAAGCGTTGTT CACAGTTCTCCAGGAAGAACAAGTCACTGTGATACTGAGGCTTGTTCATGTGCCAATAAATGTGAGAGCA GCCCATGTGAAAGGAAGAGGAGGTCGTATGGATCACATAATACTGATGAAGATATGGAACCGGACTGGTT AACTTGGCCTGCGGGTACGCTGCACTGTACTGAATGTGTTGTTTGCCTTGAGAATTTTGAAAATGGATGT TTGCTAATGGGGTTGCCTTGTGGTCACGTGTTTCACCAGAATTGCATTGTTATGTGGTTGGCTGGGGGCC GACACTGTTGCCCTGTTTGTCGTTGGCCTTCATATAAGAAAAAGCAGCCATATGCACAACAACAGCCGTT GTCAAATGATGTTCCATCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222470 representing NM_009543
Red=Cloning site Green=Tags(s) MWLKLFFLLLYFLVLFVLARFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRE LVEKSGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQVIANDRSPLVGKIHWEKM VKKVSRFGIRTGTFNCSSDPRYCRRRGWVRSTLIMSVPQTSTSKGKVMLKEYSGRKIEVEHIFKWITAHA ASRIKTIYNVEHLKEEWNKSDQYWVKIYLFANLDQPPAFFSALSIKFTGRVEFIFVNVENWNNKSYMTDI GIYNMPSYILRTPEGIYRYGNHTGEFISLQAMDSFLRSLQPEVNDLFVLSLVLVNLMAWMDLFITQGATI KRFVVLISTLGTYNSLLIISWLPVLGFLQLPYLDSFYDYSLRLLRYSNTTTLASWVRADWMFYTSHPALF LSTYLGHGLLIDYFEKKRRRSNNDEVNANNLEWLSSLWDWYTSYLFHPIASFQNFPVDSDWDEDPDLFLE RLAFPDLWLHPLIPTDYIKNLPMWRFKCLGVQSEEEMSESSQDTENDSDSDNMDTFSSSKDIFEDKQSVV HSSPGRTSHCDTEACSCANKCESSPCERKRRSYGSHNTDEDMEPDWLTWPAGTLHCTECVVCLENFENGC LLMGLPCGHVFHQNCIVMWLAGGRHCCPVCRWPSYKKKQPYAQQQPLSNDVPS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009543 |
ORF Size | 2049 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009543.3, NP_033569.2 |
RefSeq Size | 3264 bp |
RefSeq ORF | 2052 bp |
Locus ID | 22644 |
UniProt ID | Q9R1W3 |
Cytogenetics | 6 32.16 cM |
Gene Summary | This gene encodes a member of the RING finger family of E3 ubiquitin-protein ligases. These proteins catalyze the transfer of the ubiquitin protein from a ubiquitin E2 enzyme to a protein substrate. Homozygous knockout mice for this gene exhibit enhanced anxiety-like behavior. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220426 | Rnf103 (untagged) - Mouse ring finger protein 103 (Rnf103), (10ug) |
USD 689.00 |
|
MR222470 | Rnf103 (Myc-DDK-tagged) - Mouse ring finger protein 103 (Rnf103) |
USD 625.00 |
|
MR222470L3 | Lenti ORF clone of Rnf103 (Myc-DDK-tagged) - Mouse ring finger protein 103 (Rnf103) |
USD 925.00 |
|
MR222470L4 | Lenti ORF clone of Rnf103 (mGFP-tagged) - Mouse ring finger protein 103 (Rnf103) |
USD 925.00 |
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