Rnf103 (NM_009543) Mouse Untagged Clone

CAT#: MC220426

Rnf103 (untagged) - Mouse ring finger protein 103 (Rnf103), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf103
• Synonyms: AW146237; AW212918; kf-1; Zfp10; Zfp103
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220426 representing NM_009543
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGAAGCTGTTTTTCTTGCTCCTGTATTTCCTGGTCCTGTTCGTCCTGGCCAGGTTTTTTGAGG
  CCATTGTGTGGTACGAGACTGGCATCTTTGCTACTCAGCTGGTGGATCCGGTGGCATTGAGCTTCAAGAA
  GCTGAAGACCATTCTGGAGTGTCGAGGGCTGGGCTACTCCGGGCTGCCGGAGAAGAAAGATGTTCGGGAG
  CTGGTGGAGAAGTCAGGTGACTTGATGGAAGGTGAACTCTATTCTGCTCTTAAGGAAGAAGAGGCATCTG
  AGTCTGTTTCTAGTACCAATTTCAGTGGTGAAATGCATTTCTATGAGCTTGTGGAAGACACAAAAGATGG
  CATCTGGCTGGTTCAGGTCATAGCAAATGACAGAAGTCCTTTGGTGGGTAAAATCCACTGGGAGAAAATG
  GTGAAAAAAGTGTCAAGATTTGGAATACGGACAGGCACTTTCAACTGTTCCAGTGATCCCAGGTACTGCA
  GAAGGAGAGGCTGGGTGCGTTCCACACTCATCATGTCTGTGCCACAAACAAGTACATCTAAAGGGAAAGT
  CATGCTTAAAGAGTACAGTGGACGCAAGATTGAAGTAGAGCACATTTTTAAGTGGATAACTGCCCATGCA
  GCTTCTCGGATCAAAACTATATATAATGTGGAGCATTTGAAGGAAGAATGGAATAAAAGTGATCAATACT
  GGGTAAAAATATACCTATTTGCAAACCTTGACCAACCGCCAGCTTTCTTCTCTGCATTAAGTATAAAATT
  TACTGGAAGAGTTGAGTTTATTTTTGTTAATGTGGAAAATTGGAACAACAAGAGTTATATGACAGATATT
  GGTATTTATAACATGCCATCATACATACTTAGAACTCCTGAAGGAATTTATAGATACGGAAACCACACAG
  GTGAATTTATATCCCTTCAGGCCATGGATTCATTTCTGCGCTCATTACAACCTGAAGTAAATGATCTGTT
  TGTTTTGAGTTTGGTTCTAGTTAATCTTATGGCTTGGATGGACTTATTTATTACACAAGGAGCAACCATC
  AAGCGATTTGTGGTTCTCATAAGCACTTTAGGGACATACAATTCCCTATTAATTATTTCTTGGCTACCTG
  TGTTGGGCTTTCTACAGCTCCCTTACTTAGATAGCTTTTATGACTATAGTTTAAGATTGCTGCGGTATTC
  TAATACAACCACACTGGCTTCGTGGGTAAGGGCAGACTGGATGTTTTACACTTCACACCCAGCCCTGTTT
  CTCAGTACATACCTTGGACATGGTTTGCTAATTGATTACTTTGAGAAGAAGAGACGTCGCAGCAACAATG
  ATGAAGTTAATGCGAATAATTTAGAATGGTTATCAAGTCTGTGGGACTGGTACACCAGCTACCTCTTCCA
  CCCGATTGCTTCTTTTCAGAACTTTCCTGTAGACTCTGATTGGGACGAAGACCCTGACTTATTCTTGGAA
  CGGTTAGCTTTCCCTGACCTTTGGCTTCACCCTCTGATACCAACTGATTATATTAAAAACTTACCAATGT
  GGCGATTTAAATGTCTTGGGGTTCAGTCTGAAGAAGAAATGTCAGAGAGTTCTCAAGACACTGAAAATGA
  CTCGGATAGTGACAACATGGACACTTTTAGTAGTAGTAAGGATATATTTGAAGATAAACAAAGCGTTGTT
  CACAGTTCTCCAGGAAGAACAAGTCACTGTGATACTGAGGCTTGTTCATGTGCCAATAAATGTGAGAGCA
  GCCCATGTGAAAGGAAGAGGAGGTCGTATGGATCACATAATACTGATGAAGATATGGAACCGGACTGGTT
  AACTTGGCCTGCGGGTACGCTGCACTGTACTGAATGTGTTGTTTGCCTTGAGAATTTTGAAAATGGATGT
  TTGCTAATGGGGTTGCCTTGTGGTCACGTGTTTCACCAGAATTGCATTGTTATGTGGTTGGCTGGGGGCC
  GACACTGTTGCCCTGTTTGTCGTTGGCCTTCATATAAGAAAAAGCAGCCATATGCACAACAACAGCCGTT
  GTCAAATGATGTTCCATCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009543
• Insert Size: 2052 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009543.3, NP_033569.2
• RefSeq Size: 3296 bp
• RefSeq ORF: 2052 bp
• Locus ID: 22644
• UniProt ID: Q9R1W3
• Cytogenetics: 6 32.16 cM
• Gene Summary: This gene encodes a member of the RING finger family of E3 ubiquitin-protein ligases. These proteins catalyze the transfer of the ubiquitin protein from a ubiquitin E2 enzyme to a protein substrate. Homozygous knockout mice for this gene exhibit enhanced anxiety-like behavior. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
  Transcript Variant: This variant (1) encodes the longer isoform (1).