Defb4 (NM_019728) Mouse Tagged ORF Clone
CAT#: MG222346
- TrueORF®
Defb4 (tGFP-tagged) - Mouse defensin beta 4 (Defb4), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_019728" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Defb4 |
Synonyms | 2310001F05Rik; BD-4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222346 representing NM_019728
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGATCCATTACCTTCTCTTCACATTTCTCCTGGTGCTGCTGTCTCCACTTGCAGCCTTTACCCAAA TTATCAACAATCCAATAACATGCATGACCAATGGAGCCATATGCTGGGGTCCGTGCCCTACCGCTTTTCG ACAGATTGGCAATTGTGGCCATTTTAAAGTCAGATGCTGTAAGATAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222346 representing NM_019728
Red=Cloning site Green=Tags(s) MRIHYLLFTFLLVLLSPLAAFTQIINNPITCMTNGAICWGPCPTAFRQIGNCGHFKVRCCKIR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_019728 |
ORF Size | 189 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019728.4, NP_062702.1 |
RefSeq Size | 458 bp |
RefSeq ORF | 192 bp |
Locus ID | 56519 |
UniProt ID | P82019 |
Cytogenetics | 8 A1.3 |
Gene Summary | Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to mouse (but not human) CCR6 and induce chemotactic activity of CCR6-expressing cells (PubMed:20068036).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210069 | Defb4 (untagged) - Mouse defensin beta 4 (Defb4), (10ug) |
USD 165.00 |
|
MR222346 | Defb4 (Myc-DDK-tagged) - Mouse defensin beta 4 (Defb4) |
USD 150.00 |
|
MR222346L3 | Lenti ORF clone of Defb4 (Myc-DDK-tagged) - Mouse defensin beta 4 (Defb4) |
USD 450.00 |
|
MR222346L4 | Lenti ORF clone of Defb4 (mGFP-tagged) - Mouse defensin beta 4 (Defb4) |
USD 450.00 |
{0} Product Review(s)
Be the first one to submit a review