Txnrd3 (NM_001178060) Mouse Tagged ORF Clone

CAT#: MG221636

Txnrd3 (GFP-tagged) - Mouse thioredoxin reductase 3 (Txnrd3) transcript variant 2, (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Product Images

Circular map for MG221636

Specifications

Product Data

• Type: Mouse Untagged ORF Clone
• Symbol: Txnrd3
• Synonyms: AI196535; Tg; TGR; TR; TR2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221636 representing NM_001178060
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCGCCACCCGGCCGCCGCGCCCGCCTGGCCTCCCCTGGGACCAGCCGCCCGTCTTCTGAGGCCC
  GCGAGGAGCTGCGGCGCCGCCTGCGGGACCTCATCGAGGGCAACAGGGTGATGATCTTCAGCAAGAGTTA
  CTGTCCACACAGCACGCGGGTTAAGGAACTCTTTTCGTCCTTGGGAGTGGTCTATAACATCCTGGAACTT
  GATCAAGTTGATGACGGGGCCAGTGTTCAGGAAGTGCTGACAGAAATCAGTAACCAGAAAACGGTGCCCA
  ATATTTTTGTGAATAAAGTGCACGTGGGTGGATGTGACCGAACTTTCCAGGCACATCAGAATGGTTTACT
  GCAGAAGCTCCTCCAAGATGACTCGGCTCATGATTACGACCTCATCATCATCGGCGGGGGTTCTGGCGGC
  CTCTCTTGTGCCAAGGAAGCTGCCAACTTGGGAAAGAAGGTCATGGTGCTAGACTTTGTGGTCCCATCGC
  CTCAGGGCACGACCTGGGGCCTTGGCGGCACCTGTGTGAACGTAGGCTGTATTCCAAAGAAGCTGATGCA
  TCAGGCAGCCCTCCTGGGGCATGCTTTGCAAGATGCCAAGAAATATGGCTGGGAGTATAACCAGCAGGTG
  AAGCACAACTGGGAGGCCATGACAGAAGCTATCCAGAGCCACATTGGCTCCTTGAACTGGGGCTACAGGG
  TAACCCTTCGGGAGAAAGGCGTGACCTATGTCAACTCCTTCGGGGAGTTTGTGGACCTGCATAAAATAAA
  GGTTCAACAGTTGGAGAAAGGTTTACCAGGAAAATTGAAAGTCGTGGCTAAGTCCACCGAAGGACCGGAA
  ACAGTAGAAGGGATATACAACACGGTTTTGTTAGCAATTGGTCGTGACTCCTGTACAAGGAAAATAGGGC
  TGGAGAAGATCGGGGTCAAAATCAATGAGAAGAATGGCAAAATACCAGTAAACGATGTGGAGCAGACCAA
  CGTGCCTCATGTTTATGCTATTGGGGACATACTGGACGGCAAACCAGAGCTCACCCCCGTTGCCATACAG
  GCAGGCAAGCTGCTAGCTCGAAGACTCTTTGGGGTCTCTTTAGAAAAGTGTGATTATATTAACATCCCAA
  CAACGGTGTTCACACCTCTGGAATATGGCTGTTGTGGACTGTCGGAAGAGAAAGCCATCGAAATGTATAA
  AAAAGAGAATCTGGAAGTGTATCACACCTTGTTTTGGCCTCTCGAGTGGACAGTTGCTGGCAGAGACAAC
  AACACCTGTTATGCAAAGATAATCTGCAACAAATTCGACAACGAACGTGTGGTGGGATTTCACCTTCTGG
  GGCCAAATGCTGGTGAAATCACGCAGGGATTTGCAGCTGCAATGAAATGTGGGCTCACGAAGCAGCTACT
  GGATGATACCATTGGAATCCACCCCACCTGTGGTGAGGTATTCACAACATTGGAAATCACAAAGTCCTCA
  GGGCTGGACATTACTCAGAAAGGCTGCTGAGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221636 representing NM_001178060
  Red=Cloning site Green=Tags(s)
  
  MSSPPGRRARLASPGTSRPSSEAREELRRRLRDLIEGNRVMIFSKSYCPHSTRVKELFSSLGVVYNILEL
  DQVDDGASVQEVLTEISNQKTVPNIFVNKVHVGGCDRTFQAHQNGLLQKLLQDDSAHDYDLIIIGGGSGG
  LSCAKEAANLGKKVMVLDFVVPSPQGTTWGLGGTCVNVGCIPKKLMHQAALLGHALQDAKKYGWEYNQQV
  KHNWEAMTEAIQSHIGSLNWGYRVTLREKGVTYVNSFGEFVDLHKIKVQQLEKGLPGKLKVVAKSTEGPE
  TVEGIYNTVLLAIGRDSCTRKIGLEKIGVKINEKNGKIPVNDVEQTNVPHVYAIGDILDGKPELTPVAIQ
  AGKLLARRLFGVSLEKCDYINIPTTVFTPLEYGCCGLSEEKAIEMYKKENLEVYHTLFWPLEWTVAGRDN
  NTCYAKIICNKFDNERVVGFHLLGPNAGEITQGFAAAMKCGLTKQLLDDTIGIHPTCGEVFTTLEITKSS
  GLDITQKGC*G
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178060
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178060.1, NP_001171531.1
• RefSeq Size: 2494 bp
• RefSeq ORF: 1506 bp
• Locus ID: 232223
• Cytogenetics: 6 D1
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is evidence for additional isoforms resulting from the use of a non-AUG (CUG), and an in-frame downstream AUG as translation initiation codons (PMID:20018845). [provided by RefSeq, Aug 2017]