Mia2 (NM_177321) Mouse Tagged ORF Clone
CAT#: MG219873
- TrueORF®
Mia2 (tGFP-tagged) - Mouse melanoma inhibitory activity 2 (Mia2), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_177321" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Mia2 |
Synonyms | Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG219873 representing NM_177321
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGTCAGTGTTCAAAGAATCCTTCTTTTGGTTGTTTCTCTGGCCAAGTGTCTGGAGGGTACAA AGTTGCTGGCACACCTTAAGAAGTGTGGTGACTTGGAATGTGAAACTTTGATCAGCCGAGTCTTAGCCCT GAGAGATTACACAGGACCCGACTGTCGGTACCTGAACTTCACTACGGGAGAAGAGATATCTGTTTATGTT AAACTTGGAGGAGACAGAGAAGATTTGTGGGCAGGAAGCAAAGGAAAAGACTTTGGATTTTTTCCCAGAG ATGCAGTCGAGATTGAAGAGGTGTTCATATCTGAAGAAGTCGAAATGCCAACTAAATCTGACTTTCTTTG TCTTCTTGGAGAAGGCTACATATTTGGAAGTGAACAGAGTGAATTAAACAGTGAAGATGATGAAGAACAT ATGTACCCATATGAAAAAGATGAAGACCAAAACTATAATATATATGAGGGTGATTTTCAGCCAGAACCTG ACTTATATGCAGCTGCTGAAGGGACTTTGTTGGAGGACCAAATTCCAGCATCCGAAGCTCCTGATGATTT CCGATTCTCCAGTGAGTGGAAGGCCTGGGAAGGGGCTGGAAGCCAGGGTGGAGGGGAGCAGGATTACACT GCAGACTCTGACCAAGACTTGCCATCCCTCAGTAAGCCAGAAAGGCAAGGATGGTTTGGCCTGGGGACAG AAGAAGCTGAAGAGAAGGTTTTCGAATCAGATACTGAACCTACACAAGAATTAGCACTAGAAGAGGAGAG TGACCTGGAGAAATTACACAGTGGCGAACCCCAAGTGGAACTTGAGCAAGAGCCAAAATCAGAGACATTA GAATTCAGTTCAGTGCCGGACGAAGAGTATGAGCTAGAATCTGAGACGGAGAGTATCCTCAAACCCCAAG CTTCTGGCTGGTTTGGTGAGGGCCTTACAAGTTATTTAGGTTTTGGAAATGAGGAGGCAGGACTTGAGTT ATTGTCCAAAGAAAGCAATCCACCATTACAAGATATTCCCAGCTCTGTTCCACCAGATGAAGAAGTCCCG GCTCCATGCAGAGAAATCTCAACAGACAAGGAAGATGCAGTCATTAATGATAGCTCGGTTCTCAGTCCAA GCTGGTTTTACTATGGATTTGGTATGCTAGGCTTTACAAATGCCGACGAAGACAACATTGTTTCAGACAA AGGAGAAAATGAAGATGGTGAGGTAGATAACCTCAAACATCCTATAGGAAGTGACTTTGACCCTGAAAAG GAACAAGAAAGGAAAATAGTAACTGTGGAAACCGAAGACCAGGCAGGTACAGAAAGCGTCTTGGAGAAGA CAGACGAGTCTGGTTCCATGCAGTATCTGAAGAAGTTCTTTGATAATCCTTGGGGCTTCCAGAGTCTCCC AGAGGACACAGAATTACCATTTTCCAAAAAGATGCTGGATCAAGATGATATAGTAGAAAATGACAAAATT GAAGAACTTTCCACTGAAAATTCTCCCACAGGTAGCATGAAAGACCCCGTGATGCTGGCGAGCAGATACG TTCTGTCAGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219873 representing NM_177321
Red=Cloning site Green=Tags(s) MAEVSVQRILLLVVSLAKCLEGTKLLAHLKKCGDLECETLISRVLALRDYTGPDCRYLNFTTGEEISVYV KLGGDREDLWAGSKGKDFGFFPRDAVEIEEVFISEEVEMPTKSDFLCLLGEGYIFGSEQSELNSEDDEEH MYPYEKDEDQNYNIYEGDFQPEPDLYAAAEGTLLEDQIPASEAPDDFRFSSEWKAWEGAGSQGGGEQDYT ADSDQDLPSLSKPERQGWFGLGTEEAEEKVFESDTEPTQELALEEESDLEKLHSGEPQVELEQEPKSETL EFSSVPDEEYELESETESILKPQASGWFGEGLTSYLGFGNEEAGLELLSKESNPPLQDIPSSVPPDEEVP APCREISTDKEDAVINDSSVLSPSWFYYGFGMLGFTNADEDNIVSDKGENEDGEVDNLKHPIGSDFDPEK EQERKIVTVETEDQAGTESVLEKTDESGSMQYLKKFFDNPWGFQSLPEDTELPFSKKMLDQDDIVENDKI EELSTENSPTGSMKDPVMLASRYVLSG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177321 |
ORF Size | 1551 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177321.2, NP_796295.1 |
RefSeq Size | 1628 bp |
RefSeq ORF | 1554 bp |
Locus ID | 338320 |
UniProt ID | Q91ZV0 |
Cytogenetics | 12 C1 |
Gene Summary | This gene encodes a protein that is involved in endoplasmic reticulum-to-Golgi trafficking and regulation of cholesterol metabolism. Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species. Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding diverse isoforms. A mutation in this gene (couch-potato or cpto) may result in low levels of plasma cholesterol and triglycerides. [provided by RefSeq, Sep 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217381 | Mia2 (untagged) - Mouse melanoma inhibitory activity 2 (Mia2), (10ug) |
USD 530.00 |
|
MR219873 | Mia2 (Myc-DDK-tagged) - Mouse melanoma inhibitory activity 2 (Mia2) |
USD 481.00 |
|
MR219873L3 | Lenti ORF clone of Mia2 (Myc-DDK-tagged) - Mouse melanoma inhibitory activity 2 (Mia2) |
USD 781.00 |
|
MR219873L4 | Lenti ORF clone of Mia2 (mGFP-tagged) - Mouse melanoma inhibitory activity 2 (Mia2) |
USD 781.00 |
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