Rgs20 (NM_021374) Mouse Tagged ORF Clone

CAT#: MG219776

Rgs20 (tGFP-tagged) - Mouse regulator of G-protein signaling 20 (Rgs20) transcript variant 2, (10ug)

Product Images

Circular map for MG219776

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rgs20
• Synonyms: 2900073E09Rik; Rgsz1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219776 representing NM_021374
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCACGGCCAACGGAGGCCCGAGGGCCAGAGCTTCCCCTTCGGCTAGCCCAGCGGACCCTGGCCTGC
  CTGAGGGATCTGAGCGGACGGAAATGCGGATGCGGCAGATGTGCGGCGGAAGTGAGACCCAGGGCCCTGC
  CCCAAGCCAGCAGGGTGGCCGGGGCTCCAACGCCTGCTGCTTCTGTTGGTGCTGCTGCTGCACCTGTTCT
  TGCCTCACTGTCAGAAACCAAGAAGACCAGAGACCCCAAAGAGCCTCCCATGAAATCAGAACAGACATTC
  CAGCATGTGAAGAAAGCCCCACGCCTACTCTGGAAGAAGTCTGTGCCTGGGCCCAGTCTTTTGATAACCT
  GATGGTCACTCCAGCCGGAAGAAATGCATTCCGGGAATTTCTTCGAACAGAATTCAGTGAAGAAAACATG
  CTCTTCTGGATGGCCTGTGAAGAACTGAAAAGGGAAGCTAATAAAAGCACTATTGAGGAGAAAGCAAGGA
  TAATCTATGAGGACTATATTTCTATTCTCTCTCCTAAAGAGGTCAGCTTGGACTCCCGAGTACGAGAGGT
  TATCAACAGAAACATGGTGGATCCATCCCAGCACATATTCGATGACGCTCAACTGCAGATCTACACTCTA
  ATGCACAGAGACTCCTATCCCCGCTTCATGAACTCCACAGTCTATAAGGATTTGCTTACGTCCTTAGCTG
  AGAAAACAGTTGAAGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219776 representing NM_021374
  Red=Cloning site Green=Tags(s)
  
  MRTANGGPRARASPSASPADPGLPEGSERTEMRMRQMCGGSETQGPAPSQQGGRGSNACCFCWCCCCTCS
  CLTVRNQEDQRPQRASHEIRTDIPACEESPTPTLEEVCAWAQSFDNLMVTPAGRNAFREFLRTEFSEENM
  LFWMACEELKREANKSTIEEKARIIYEDYISILSPKEVSLDSRVREVINRNMVDPSQHIFDDAQLQIYTL
  MHRDSYPRFMNSTVYKDLLTSLAEKTVEA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021374
• ORF Size: 717 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021374.5, NP_067349.2
• RefSeq Size: 1665 bp
• RefSeq ORF: 720 bp
• Locus ID: 58175
• UniProt ID: Q9QZB1
• Cytogenetics: 1 A1
• Gene Summary: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.[UniProtKB/Swiss-Prot Function]