Cd84 (NM_013489) Mouse Tagged ORF Clone

CAT#: MG219598

Cd84 (tGFP-tagged) - Mouse CD84 antigen (Cd84), (10ug)

Product Images

Circular map for MG219598

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cd84
• Synonyms: A130013D22Rik; CDw84; SLAMF5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219598 representing NM_013489
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGCGCCATCTGTGGATCTGGTTCCTTTGCCTACAAACCTGGTCTGAAGCAGCAGGAAAAGATG
  CAGACCCGATGGTAATGAATGGGATTCTTGGGGAGTCAGTTACTTTCCTCTTAAATATTCAAGAACCAAA
  GAAAATTGACAACATTGCCTGGACTTCTCAATCATCTGTTGCTTTTATAAAACCAGGAGTCAATAAAGCT
  GAAGTTACCATAACCCAGGGCACTTATAAAGGACGAATAGAAATCATAGATCAGAAGTATGACCTGGTCA
  TTAGAGACCTGAGGATGGAAGATGCAGGAACTTACAAAGCAGACATCAATGAAGAGAATGAGGAAACCAT
  CACCAAGATCTACTACCTTCATATCTACCGTCGACTTAAAACACCAAAAATTACACAGAGTTTGATATCA
  TCTTTGAACAATACCTGTAATATCACACTGACATGCTCTGTGGAAAAGGAAGAAAAGGATGTCACATATA
  GCTGGAGTCCCTTTGGAGAGAAAAGCAATGTCCTTCAAATCGTCCACTCCCCCATGGACCAAAAACTGAC
  CTACACATGTACAGCCCAGAACCCTGTCAGCAACAGTTCTGACTCTGTCACTGTCCAGCAGCCATGTACA
  GACACTCCAAGCTTCCATCCTCGCCATGCTGTGTTGCCAGGAGGATTGGCCGTGCTCTTTCTGCTTATTC
  TCATTCCGATGTTGGCATTTCTGTTCCGTTTGTACAAGAGAAGGCGAGACAGGATTGTCCTGGAAGCAGA
  TGATGTCTCAAAGAAAACAGTATATGCTGTAGTTTCAAGAAATGCTCAACCCACAGAGTCCAGAATCTAT
  GATGAAATCCCTCAGTCCAAGATGCTGTCCTGTAAGAAAGATCCGGTGACCACCATTTATTCCTCAGTGC
  AGCTTTCTGAGAAGATGAAGGAAACCAACATGAAGGACAGAAGTCTGCCTAAGGCTTTGGGTAATGAAAT
  TGTTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219598 representing NM_013489
  Red=Cloning site Green=Tags(s)
  
  MAQRHLWIWFLCLQTWSEAAGKDADPMVMNGILGESVTFLLNIQEPKKIDNIAWTSQSSVAFIKPGVNKA
  EVTITQGTYKGRIEIIDQKYDLVIRDLRMEDAGTYKADINEENEETITKIYYLHIYRRLKTPKITQSLIS
  SLNNTCNITLTCSVEKEEKDVTYSWSPFGEKSNVLQIVHSPMDQKLTYTCTAQNPVSNSSDSVTVQQPCT
  DTPSFHPRHAVLPGGLAVLFLLILIPMLAFLFRLYKRRRDRIVLEADDVSKKTVYAVVSRNAQPTESRIY
  DEIPQSKMLSCKKDPVTTIYSSVQLSEKMKETNMKDRSLPKALGNEIVV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013489
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013489.2, NP_038517.1
• RefSeq Size: 3190 bp
• RefSeq ORF: 990 bp
• Locus ID: 12523
• UniProt ID: Q18PI6
• Cytogenetics: 1 79.54 cM
• Gene Summary: Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (PubMed:20962259). Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B (PubMed:20962259). Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells (By similarity). Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation (PubMed:20153220). In germinal centers involved in maintaining B cell tolerance and in preventing autoimmunity (PubMed:25801429). In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1 (By similarity). In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2 (PubMed:20628063). Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8; inhibits TRIM21-mediated proteasomal degradation of IRF8 (By similarity).[UniProtKB/Swiss-Prot Function]