Tacc1 (NM_177089) Mouse Tagged ORF Clone

CAT#: MG219321

Tacc1 (tGFP-tagged) - Mouse transforming acidic coiled-coil containing protein 1 (Tacc1) transcript variant 1, (10ug)

Product Images

Circular map for MG219321

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tacc1
• Synonyms: 4833447E04Rik; AA960202; B230378H13Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219321 representing NM_177089
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCAGTCCCTGGCAGATCCTGTCGCCCGTGCAGTGGGCCAAGTGGACGTGGTCCGCGGTGCGCG
  GTTCGGGCGCCGGAGAGGACGAAGCCGGAGGTCCCGAGGGCGACCCCGAGGAGGAAGAGGACTCGCAAGC
  CGAGACCAAATCCCTGAGTTTCAGCTCGGATTCTGAAGGTAATTTTGAGACTCCTGAAGCTGAAACTCCA
  ATCAGATCGCCTCTCAAGGAGTCTTGTGATTCATCACCGGGATTGGCAGAACCCGAGGCCAAACCCCAAG
  AATCTCGAGAAGCCGATGAACAGTTGGTAGCAGAAGTAATTGAAAAATGTTCACCCGATACATGTTCTCG
  ATCTTCAGAAAATGAAGCGCCACAGGCGACCGTCGATTCTCACCCAGTCAAGGATGTCAGAGGGAAGGCG
  GAACACGATGTTAGCAAGATCTCAGTAGTGAGGCCGTTTTCAATAGAAACCAGGAATTGTACAGATGACC
  CTGCGGCTCTGGGGACAGCAGCAGCCCATGGCTGTGTCCCCGTGCTCCCAGGCATGGCCTTGCCTTCCAC
  CACACCGGAAGCCACTCAGGATGAGCCAGTGATGGACAGAGGCATGGGGGTTACATTGGAGGCTTTTACA
  GAAGCCAGTCTGAAGACTGGAGGACCCTGTCCAGAGCCCGTGGCCAGCAGAAGCAAGCTAAGAAAGCCCA
  AACCTGTCTCCCTGAGGAAGAAGATGGCCCCTGAACCGGAAATGTTGATGGAGGGTTCACCTCTCCCTAA
  GGCTTCCTCCCCATGGCTTCCTGATGGCTTGGATCAGAACGCGAATCCTAGTGTGCTGAGAGGCTCGGGG
  GCCCAGAGATCTCCCCTTAACCTTAAAGAAACAGCTGGTGTTCTCAGTAACGACACCAGTGACTCGGGGG
  TCGAGCTGCAGGGTGGGTCCAGGGACCCACCTCTCCAACTTGAGGATGATTTTACAGAAGATGGGGAGAA
  TGTCAAGATCAGGAGCGCCCTTCCGAAGCAATCTGGCAGGAAACCCAGTAACAAACTGGCACCCAGTATA
  CGAAAAGATGGCGTCAGTAAGCCAGTAGGTGTTGAGCAGCCTTCAGACCCAACAGTTCAAGATGCCTTGC
  TCGACCAGATGTCTCCTAAGCTAGATCCGAGTAAACGGAGTCATCCCCCCGCTAATTTCTTCGGGAGTGG
  CCCCATTCTGCAGAACTCCCCACCCCTCTCCTCCAAATGCTCCCACCACTTTGACCCAAATAACATTAAC
  ACTGATGACTCCGGGGATCCCTGCAAGCCAACCCCAGCTTTGACAAGCAGCGGCTTTTGTCCCGCCACTG
  GTAATCATGTTAATGAAATCTTAGACTCACCCAAGAAGGCAAAGTCACGTTTAATAACGAGCGGCTGCAA
  AGTGAAGAAATACGAAGCTCAGCCTCTCGATTTGGACGCGTGTTCCCAGGATGAAGGAGCCGTGATCTCG
  AAGATCTCAGAGATTCCCAACAGGGACGGCCACGCGACTGATGAGGAGAAGCTGGCCTCCACGTCGTCGT
  GTGCTCAGAAGTCAGCGGGGGCTGGGGTCAAAGGCATAGAAAAGGAGACATGCCAGAAGATGGAGAAAGA
  GGAACTGGCTGTGCATGGACTGCTGGAGTCCTCTTCAGAAAAGGCCCCCGTGTCTGTGGCCTGTGGGGGT
  GAGAGCCCCCTGGATGGGATCTGCCTCAGCGAAGCAGACAAGACTGCCGTGCTCACGTTGATCAGAGAAG
  AGATAATCACTAAAGAGATCGAAGCAAATGAATGGAAGAAGAAATACGAAGAGACCCGGGAAGAAGTTCT
  GGAGATGAGGAAAATTGTGGCAGAATATGAAAAGACCATTGCCCAAATGATTGAGGACGAGCAGAGGACA
  AGCATGAGTTCTCAGAAGAGTTTCCAGCAGCTGACCATGGAGAAGGAGCAGGCGCTGGCGGACCTGAACT
  CTGTGGAGAGGTCCCTTTCTGATCTCTTCAGGAGATACGAAAACCTGAAAGGCGTGCTAGAAGGATTCAA
  GAAGAATGAAGAAGCCTTGAAAAAGTGTGCTCAGGACTACTTAGCCAGAGTCAAGCAGGAAGAGCAGCGA
  TACCAGGCCCTGAAGGTTCACGCAGAGGAAAAGCTGGACAGAGCCAACGAGGAGATTGCTCAGGTTCGCT
  CCAAGGCGAAGGCTGAGAGTGCCGCTCTCCATGCCGGACTCCGGAAGGAGCAGATGAAGGTGGAGTCCCT
  GGAGAGGGCTCTTCAGCAGAAGAATCAAGAGATTGAGGAGCTGACAAAAATCTGTGACGAGTTGATCGCA
  AAGCTGGGAAAGACCGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219321 representing NM_177089
  Red=Cloning site Green=Tags(s)
  
  MAFSPWQILSPVQWAKWTWSAVRGSGAGEDEAGGPEGDPEEEEDSQAETKSLSFSSDSEGNFETPEAETP
  IRSPLKESCDSSPGLAEPEAKPQESREADEQLVAEVIEKCSPDTCSRSSENEAPQATVDSHPVKDVRGKA
  EHDVSKISVVRPFSIETRNCTDDPAALGTAAAHGCVPVLPGMALPSTTPEATQDEPVMDRGMGVTLEAFT
  EASLKTGGPCPEPVASRSKLRKPKPVSLRKKMAPEPEMLMEGSPLPKASSPWLPDGLDQNANPSVLRGSG
  AQRSPLNLKETAGVLSNDTSDSGVELQGGSRDPPLQLEDDFTEDGENVKIRSALPKQSGRKPSNKLAPSI
  RKDGVSKPVGVEQPSDPTVQDALLDQMSPKLDPSKRSHPPANFFGSGPILQNSPPLSSKCSHHFDPNNIN
  TDDSGDPCKPTPALTSSGFCPATGNHVNEILDSPKKAKSRLITSGCKVKKYEAQPLDLDACSQDEGAVIS
  KISEIPNRDGHATDEEKLASTSSCAQKSAGAGVKGIEKETCQKMEKEELAVHGLLESSSEKAPVSVACGG
  ESPLDGICLSEADKTAVLTLIREEIITKEIEANEWKKKYEETREEVLEMRKIVAEYEKTIAQMIEDEQRT
  SMSSQKSFQQLTMEKEQALADLNSVERSLSDLFRRYENLKGVLEGFKKNEEALKKCAQDYLARVKQEEQR
  YQALKVHAEEKLDRANEEIAQVRSKAKAESAALHAGLRKEQMKVESLERALQQKNQEIEELTKICDELIA
  KLGKTD
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177089
• ORF Size: 2328 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177089.5, NP_796063.3
• RefSeq Size: 7695 bp
• RefSeq ORF: 2331 bp
• Locus ID: 320165
• UniProt ID: Q6Y685
• Cytogenetics: 8 A2
• Gene Summary: Involved in transcription regulation induced by nuclear receptors, including in T3 thyroid hormone and all-trans retinoic acid pathways. Might promote the nuclear localization of the receptors (By similarity). Likely involved in the processes that promote cell division prior to the formation of differentiated tissues.[UniProtKB/Swiss-Prot Function]