Chml (NM_021350) Mouse Tagged ORF Clone

CAT#: MG219136

Chml (tGFP-tagged) - Mouse choroideremia-like (Chml), (10ug)

Product Images

Circular map for MG219136

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chml
• Synonyms: AI450814; E030003F13Rik; REP-2; Rep2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219136 representing NM_021350
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGAAGCTTCCCACAGAGTTTGATGTGGTAATAATAGGGACAGGCTTGCCGGAGTCCATCCTGG
  CAGCTGCATGTTCAAGAAGTGGACAGAGAGTTCTGCATGTTGACTCAAGGAGTTACTATGGAGGGAATTG
  GGCGAGTTTCAGCTTTACAGGTTTGCAGTCCTGGCTGAAGGACTATCAACAGAACCATGACAGCGAGGAA
  GGCGTGACCGCCACATGGCAAGACCTGATCCATGAAACAGAAGAAGCCATCTCTCTTCGCAAGAAGGATG
  AAACTATTCAGCACACAGAAGTCTTCTGTTATGCTAGTCAAGATGTGGAGGACAGCGTGCAAGACACCGA
  GACTCTGCAGAGAAGTTCCCCCCTAGAGGCATCTGCTACTCCGGCTGACTCTCTGGATTCTGCAAGCTTG
  CCCAAAGAAAGGCAGTCAGCTTACTCTACAAGCTATGAAGTGCCTTCTAGACATACAGAGGAAAGTGATA
  GAGAGCTTTCACTACCCTCAGCTAATGTGGAGGACTCACTGGAGAAAGAAAAGTACTGTGGCGATAAGAC
  GGATATGCACACAGTTTCAGGTGAAGATAAAGGTGAACACAAACTTGTTGTACAAGACAGCATTGAACAA
  CCAAAGAGAAATAGGATTACTTACTCTCAAATGGTTAAGGAGAGCCGGAGATTTAATATTGACTTGGTAT
  CAAAGCCGCTGTATTCTCAAGGGTCACTGATTGATCTTCTAATCAAATCAAATGTTAGTCGTTATGCAGA
  ATTTAAGAATGTCACTAGGATCCTTGCATTTTGGGAAGGGAAGGTAGAACAGGTGCCTTGCTCCAGAGCA
  GATGTCTTTAATAGTAAAGAGCTCAGTATGGTTGAAAAGAGAATGCTAATGAAGTTTCTTACATTCTGTT
  TAGACTATGAACAACATTCTGATGAATACCAAGATTTCAAACAGTGTTCATTTTCTGACTACTTAAAAAC
  TAAAAAACTAACACCTAATCTCCAGCACTTCATACTACACTCAATTGCAATGACATCAGAATCATCTTGC
  ACTACATTAGATGGCCTTCAAGCAACAAAAACCTTTCTTCAGTGTCTTGGACGGTTTGGCAATACTCCCT
  TTATATTTCCTTTATACGGCCACGGAGAAATTCCCCAGTGTTTCTGCAGGATGTGTGCAGTTTTTGGTGG
  AGTCTATTGTCTTCGCCATAAAGTACAATGCCTTGTAGTTGACAAAGACTCTGGAAGATGTAAAGGAATC
  ATAGATGCCTTTGGTCAGAGAATAAGTGCCAACTACTTTATTGTGGAGGACAGTTATCTTCCTAAGGAAA
  CATGTTCAAACGTGCAATATAAGCAGATCTCAAGGGCAGTGCTCATCACAGATCAGTCCATCCTGAAGAC
  AGATTCGGACCAGCAGATTTCCATTTTGGTAGTGCCTCCTCTGGAGCCAGGAACCACTTCTGTTCGGGTC
  ATGGAATTATGTTCGTCAACCATGACATGCATGAAGGACAGCTATCTGGTCCACCTGACCTGTTCCTCTT
  CAAAAACAGCCAGAGAAGACTTGGAACCTGTGGTGAAGCAGTTATTCATTCCTGAAGCAGAAGCAGAAGC
  AGGCAAAGATGAACTCCGAAAACCAAGACTCTTGTGGGCTCTTTATTTTAATATGAGAGATTCCTCAGGA
  GTGAGCCGAAGCTCATACTGTGGCTTACCTTCCAATGTGTACATCTGCTCTGGGCCTGACTGGGGACTGG
  GGAGTGAGCATGCAGTCAAGCAGGCGGAAACACTGTTCCAAGAGATTTTTCCCAGTGAAGAGTTCTGCCC
  TCCTCCACCAAACCCTGAAGACATCATCTTTGAAGCTGAGGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219136 representing NM_021350
  Red=Cloning site Green=Tags(s)
  
  MAEKLPTEFDVVIIGTGLPESILAAACSRSGQRVLHVDSRSYYGGNWASFSFTGLQSWLKDYQQNHDSEE
  GVTATWQDLIHETEEAISLRKKDETIQHTEVFCYASQDVEDSVQDTETLQRSSPLEASATPADSLDSASL
  PKERQSAYSTSYEVPSRHTEESDRELSLPSANVEDSLEKEKYCGDKTDMHTVSGEDKGEHKLVVQDSIEQ
  PKRNRITYSQMVKESRRFNIDLVSKPLYSQGSLIDLLIKSNVSRYAEFKNVTRILAFWEGKVEQVPCSRA
  DVFNSKELSMVEKRMLMKFLTFCLDYEQHSDEYQDFKQCSFSDYLKTKKLTPNLQHFILHSIAMTSESSC
  TTLDGLQATKTFLQCLGRFGNTPFIFPLYGHGEIPQCFCRMCAVFGGVYCLRHKVQCLVVDKDSGRCKGI
  IDAFGQRISANYFIVEDSYLPKETCSNVQYKQISRAVLITDQSILKTDSDQQISILVVPPLEPGTTSVRV
  MELCSSTMTCMKDSYLVHLTCSSSKTAREDLEPVVKQLFIPEAEAEAGKDELRKPRLLWALYFNMRDSSG
  VSRSSYCGLPSNVYICSGPDWGLGSEHAVKQAETLFQEIFPSEEFCPPPPNPEDIIFEAEG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021350
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021350.2, NP_067325.2
• RefSeq Size: 6117 bp
• RefSeq ORF: 1866 bp
• Locus ID: 12663
• UniProt ID: Q9QZD5
• Cytogenetics: 1 81.7 cM
• Gene Summary: Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family (By similarity).[UniProtKB/Swiss-Prot Function]