Ccdc138 (NM_001162956) Mouse Tagged ORF Clone

CAT#: MG218677

Ccdc138 (tGFP-tagged) - Mouse coiled-coil domain containing 138 (Ccdc138), (10ug)

Product Images

Circular map for MG218677

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ccdc138
• Synonyms: 6230424H07Rik; BC042726
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218677 representing NM_001162956
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCAGGGTGGTCAAGCCACCGGGACAGGATATGGTAGTGGAGCGTCTCAAAAGCCGCTACGGCC
  TGGCGGGAAGGTGCCCCGTGGAGGAAAACGACATGACTGGTGTGTGGGCTGCTCTGATGAATCAACAGCA
  TGAACTTTCAGATTTTGACCAGACTAAATATAAAAGAAGAATTGTGACCTCCCCAGATGGTTTAGATACT
  TACAGCTCTGGAGATAAAGTGGGTTCCTCACCAAGATATTACTCTGATGGAAGGAATCATCCGACTCCAC
  CTTTCTGCAGTTCCTTCAAACATTTAAATGTGAACTGCCTAGATGATGAACTGGATTCTTTTCATGATTT
  AAAGAAATGGGAAACAGAAAAGGAATTAATGGAAGATGATCATAGAGATGGTGCCTCCAAAATAACAAAG
  CAGTCTTTCAAAGAAATGGAAACCGATGCCTTGATGACTAGCATGGCCTCAGGCCTTGAGACTGAATGCT
  GCAGTGGTTCCATTGACAGTCCTCTGAAGCAGGCCGTGTACCCAAGACCCAAAGTGTCAAAGAAGCAGGG
  TTTGCTTCCACATGAAATAAATCAAATATATGATGAATTATACCATATACATATGAAACTGCAGTATGAA
  ACTACAGCACAGAAGAAATTTGCAGAAGAACTTCAAAAACGAGAACAGTTTTTAGCAGAACGAGAACAGC
  TGCTTTTCAGCCATGAAACTGCCTTGAGCAAAATTAAAGGTGTGAAAGAAGAGGTTCTGACAAGATTTCA
  GATTTTAAAGGAGCAACATGGTACGGAAATTGAACACTTAACTGAAGCTCTTAAGGAAAAGAATAAAGAG
  AACAAGAGAATGAGGTCCTCCTTTGATACCCTGAGAGAGCTGAATGATAACTTAAGAAAACAGCTAAATG
  AAGTAAGTGAGGAAAACAAGAAGATGGAGATTCAGGCCAAAAGGGTCCAAGCTCGTTTAGATAACTTACA
  GAGGAAGTATGAGTTTATGACAGTGCAGAGACTGAAAGGAGACTCCCATGCTGCTCATGAAGTGAAGAGT
  TCAAAACAAGAAAAGGCCCCAGCTCCAAAACCCTTCAAGGCAGCACTCAATGGACAAGTGTACGAGCTGC
  TCACTGTTTTCATGGATTGGATATCAGACTGCCACCTTAGCAAAGTGGAGCCTGAGGAACCTGGTGTAGA
  TGGGGGAAAGCCACCAGCCAAGCCTTCCCAGAGAAGTGACATTCAAGAGAAATGTGTAAAGCTGTTGCCT
  ATGATGACAGAGCAGCTCCAGTGGATGCCGCTAGTGAGCGCCAAACTGCACGAGCCATTCGTAAGGTTTA
  TCTATTGGTCTCTGAGGCAGCTGGATGCCAGCGCACGGCAATCAACTATGGCATCAACATTGAGGAGATT
  GGGGGAAGACGTATTTAAAGGAGTTACAATGAAAGGAACTCAGGATAATTCTTTAGAGCATTCTGTGGAG
  AACAAAGCAAAGACAGCAGTGTTCTTCAAGAGCTCCAGCTTGCCACTGAGATTCTTATCAACCTTAATTG
  TCCTCAGAACGGTCACTCAAGCTGACTACCTAGCTCAAGCGTTTGACTCCCTTTGTTTGGACTTGAAGAC
  AGATGAAGGAAAAACCTTATTTTTGGAGTACCAAGCCATTCCAGTAATCCTAAAACACCTGAGAATATCC
  AGTAAAGGGCTCTTATCCAATGTCATCGACAGCCTGCTTCAGATGACAGTAGAGTCAAAGTCCCTGCAGC
  CATTCCTGGAAGCCTGTAGTAACTCTTTGTTTTTTCGTACTTGTTCTGTGCTGCTGCGAACGCCGAAACT
  TGATCTTCACATCCTGGAAAAGCTCAGCATCATCCTACAGAAGCTTTCCAAAATCAAGAGTAACAAGAAG
  CTCTTTGAAGTTTTTACCATTCACCTAATGCTTCAGGAAATACAAAGGACAACACATCCAGAGCACGCCT
  TTCTCTGTATTAATCTGAATTCAACTCTGTTCAACCTGGGTTTGACAAAATGTAACTCCCTGGTCACTAG
  TACAAGCCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218677 representing NM_001162956
  Red=Cloning site Green=Tags(s)
  
  MERRVVKPPGQDMVVERLKSRYGLAGRCPVEENDMTGVWAALMNQQHELSDFDQTKYKRRIVTSPDGLDT
  YSSGDKVGSSPRYYSDGRNHPTPPFCSSFKHLNVNCLDDELDSFHDLKKWETEKELMEDDHRDGASKITK
  QSFKEMETDALMTSMASGLETECCSGSIDSPLKQAVYPRPKVSKKQGLLPHEINQIYDELYHIHMKLQYE
  TTAQKKFAEELQKREQFLAEREQLLFSHETALSKIKGVKEEVLTRFQILKEQHGTEIEHLTEALKEKNKE
  NKRMRSSFDTLRELNDNLRKQLNEVSEENKKMEIQAKRVQARLDNLQRKYEFMTVQRLKGDSHAAHEVKS
  SKQEKAPAPKPFKAALNGQVYELLTVFMDWISDCHLSKVEPEEPGVDGGKPPAKPSQRSDIQEKCVKLLP
  MMTEQLQWMPLVSAKLHEPFVRFIYWSLRQLDASARQSTMASTLRRLGEDVFKGVTMKGTQDNSLEHSVE
  NKAKTAVFFKSSSLPLRFLSTLIVLRTVTQADYLAQAFDSLCLDLKTDEGKTLFLEYQAIPVILKHLRIS
  SKGLLSNVIDSLLQMTVESKSLQPFLEACSNSLFFRTCSVLLRTPKLDLHILEKLSIILQKLSKIKSNKK
  LFEVFTIHLMLQEIQRTTHPEHAFLCINLNSTLFNLGLTKCNSLVTSTSH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162956
• ORF Size: 2040 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162956.1, NP_001156428.1
• RefSeq Size: 2466 bp
• RefSeq ORF: 2043 bp
• Locus ID: 76138
• UniProt ID: Q0VF22
• Cytogenetics: 10 B4