Fpgs (NM_010236) Mouse Tagged ORF Clone
CAT#: MG218569
- TrueORF®
Fpgs (tGFP-tagged) - Mouse folylpolyglutamyl synthetase (Fpgs) nuclear gene encoding mitochondrial protein, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_010236" in other vectors (3)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Fpgs |
Synonyms | AA408187 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218569 representing NM_010236
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTGGGCGCGGAGCCGACTGTGCTCGACTCTGTCCCTGGCAGCTGTTTCTGCGCGTGGTGCAACGA CGGAGGGCGCGGCGCGGCGGGGGATGAGCGCGTGGCCAGCGCCACAGGAGCCGGGCATGGAGTATCAGGA TGCTGTGCGCACGCTCAACACCCTGCAAACCAATGCCAGCTACCTGGAGCAGGTAAAGCGCCAACGGAGT GACCCCCAGGCGCAGCTGGAGGCTATGGAGATGTACCTGGCACGGAGTGGACTGCAGGTGGAGGACTTGA ACCGGCTAAACATTATTCATGTCACTGGGACCAAAGGGAAGGGCTCCACCTGTGCCTTCACCGAACGGAT CCTGCGGAATTACGGCCTGAAGACCGGCTTCTTTAGCTCTCCTCACATGGTGCAGGTGCGGGAGCGGATT CGAATCAACGGGAAACCAATCAGCCCCGAGCTCTTCACCAAGCACTTCTGGTGCCTCTATAACCAGCTGG AGGAGTTCAAGGACGACAGCCATGTCTCCATGCCCTCTTACTTCCGCTTCCTCACACTCATGGCCTTCCA TGTCTTCCTCCAAGAGAAGGTGGACCTGGCAGTGGTGGAGGTGGGCATTGGCGGGGCTTTTGACTGCACC AACATCATCAGAAAGCCAGTGGTGTGTGGAGTCTCCTCTCTTGGCATTGACCACACCAGTCTACTAGGAG ATACAGTGGAGAAAATAGCATGGCAGAAAGGGGGCATCTTTAAGCCTGGTGTCCCTGCCTTCACTGTGGT GCAGCCAGAAGGTCCCCTGGCTGTGCTGAGGGATCGAGCCCAGCAGATTGGATGCCCGTTGTACCTGTGT CCGCCATTGGAAGCCCTGGAGGAGGTTGGACTGCCATTGAGCCTGGGTCTGGAGGGAGCACACCAGCGGT CTAATGCTGCCTTGGCCTTGCAGCTGGCCCACTGTTGGCTGGAGCGGCAGGACCACCAAGACATCCAGGA GCTGAAGGTATCCAGGCCAAGCATACGGTGGCAGCTGCCCCTGGCACCTGTGTTCCGCCCTACCCCTCAC ATGAGGCGTGGGCTTCGGGACACAGTGTGGCCTGGCCGGACACAGATACTCCAGCGGGGACCCCTTACCT GGTACCTGGATGGCGCCCATACCACCAGCAGTGTGCAGGCCTGTGTGCACTGGTACCGCCAGTCATTGGA GCGCAGCAAACGCACCGATGGAGGGTCCGAAGTACACATCTTGCTCTTCAACTCTACTGGTGACAGGGAC TCTGCTGCCCTGCTGAAGCTGCTGCAGCCCTGCCAGTTTGACTACGCTGTCTTCTGCCCCAACGTGACAG AGGTTTCATCCATAGGAAATGCAGACCAGCAGAACTTCACTGTGACTCTGGACCAGGTGCTGCTCCGCTG CCTCCAACACCAGCAGCATTGGAACGGCCTGGCTGAGAAACAGGCCAGCTCCAACCTCTGGAGCAGCTGC GGCCCAGACCCTGCTGGGCCAGGCTCCCTGCTGCTGGCCCCGCACCCACCTCAGCCTACTAGGACGAGCT CCCTCGTTTTCAGCTGCATCTCCCACGCCTTGCTGTGGATCAGCCAAGGCCGGGATCCCATCTTTCAGCC CCAGAGCCTTCCAAGGAATCTTCTCAACCACCCCACAGCCAACAGCGGGGCCAGCATTCTCCGTGAGGCT GCTGCCATCCATGTACTGGTTACAGGAAGCCTGCACCTGGTGGGCGGGGTTCTGAAACTGCTGGATCCCT CTATGTCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218569 representing NM_010236
Red=Cloning site Green=Tags(s) MSWARSRLCSTLSLAAVSARGATTEGAARRGMSAWPAPQEPGMEYQDAVRTLNTLQTNASYLEQVKRQRS DPQAQLEAMEMYLARSGLQVEDLNRLNIIHVTGTKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERI RINGKPISPELFTKHFWCLYNQLEEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCT NIIRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLC PPLEALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAPVFRPTPH MRRGLRDTVWPGRTQILQRGPLTWYLDGAHTTSSVQACVHWYRQSLERSKRTDGGSEVHILLFNSTGDRD SAALLKLLQPCQFDYAVFCPNVTEVSSIGNADQQNFTVTLDQVLLRCLQHQQHWNGLAEKQASSNLWSSC GPDPAGPGSLLLAPHPPQPTRTSSLVFSCISHALLWISQGRDPIFQPQSLPRNLLNHPTANSGASILREA AAIHVLVTGSLHLVGGVLKLLDPSMSQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010236 |
ORF Size | 1761 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010236.2, NP_034366.2 |
RefSeq Size | 2265 bp |
RefSeq ORF | 1764 bp |
Locus ID | 14287 |
UniProt ID | P48760 |
Cytogenetics | 2 22.09 cM |
Gene Summary | Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Dihydrofolate, tetrahydrofolate, 5,10-methylenetetrahydrofolate, 10-formyltetrahydrofolate and 5-formyltetrahydrofolate are the best substrates. Folic acid and 5-methyltetrahydrofolate can also act as substrates.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR218569 | Fpgs (Myc-DDK-tagged) - Mouse folylpolyglutamyl synthetase (Fpgs), nuclear gene encoding mitochondrial protein |
USD 601.00 |
|
MR218569L3 | Lenti ORF clone of Fpgs (Myc-DDK-tagged) - Mouse folylpolyglutamyl synthetase (Fpgs), nuclear gene encoding mitochondrial protein |
USD 901.00 |
|
MR218569L4 | Lenti ORF clone of Fpgs (mGFP-tagged) - Mouse folylpolyglutamyl synthetase (Fpgs), nuclear gene encoding mitochondrial protein |
USD 901.00 |
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