Glt8d1 (NM_029626) Mouse Tagged ORF Clone

CAT#: MG217287

Glt8d1 (tGFP-tagged) - Mouse glycosyltransferase 8 domain containing 1 (Glt8d1) transcript variant 1, (10ug)

Product Images

Circular map for MG217287

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Glt8d1
• Synonyms: 2410004H05Rik; 5430414N14Rik; AI450005
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217287 representing NM_029626
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATTCCGTAAAGTGAACATCATCATCTGGGTCCTGGCTGTTGTTCTCTTCTTACTGGTTTTGCACC
  ATAACTTCCTCAGCTTGAGCAGTTTGTTAAAGAATGACATTTCAGATTCGGGAATTGTGGGGCTTCAGCC
  CATAGACTTTGTCGCCAGTGCTCATCAGCATCCAGTGAGTGAGAGGCAAGAGGAGATTCCCGTGGTTATT
  GCTGCCTCTGAAGACAGGCTGGGTGGGACCATTGCAGCCATAAACAGCGTTCATCAAAACACGCGCTCCA
  ATGTGATGTTTTACATTGTTACATTCAACAGCACAGCAGACCATCTCCGGTCCTGGCTTAACAGTGGTTC
  CTTGAAAAGCATCAGGTACAAAATTGTAAATTTTGACACTAAACTTTTGGAAGGGAAAGTAAAGCAGGAT
  CCTGACCAGGGGGAGTCCATGAAACCATTAACCTTTGCAAGGTTCTACTTGCCAATTCTGGTTCCCAGTG
  CAAAGAAAGCCATATACATGGATGATGATGTCATTGTACAAGGTGACATACTTGCCTTGTACAATACACC
  ACTGAAGCCAGGACATGCTGCTGCGTTTTCAGAAGATTGTGACTCAGCCTCCACTAAAGTCATCATCCGG
  GGAGCAGGGAACCAGTACAATTACATTGGTTATCTTGACTATAAAAAGGAAAGAATTCGTAAACTTTCCA
  TGAAAGCCAGCACCTGTTCCTTTAATCCTGGAGTTTTTGTTGCAAACTTGACTGAATGGAAAAGACAGAA
  TGTAACTAACCAGTTGGAAAAATGGATGAAGCTCAATGTAGAAGAGGGATTATACAGCAGAACTCTGGCA
  GGCAGCATCACCACACCTCCTCTGCTTATCGTATTTTATCAGCAGCATTCCACCATTGACCCTATGTGGA
  ATGTCCGCCATCTTGGTTCCAGTGCTGGAAAACGGTACTCACCCCAGTTTGTAAAAGCTGCCAAGTTACT
  TCACTGGAATGGTCACTTCAAGCCTTGGGGAAGAACTGCTTCATATGCTGATGTTTGGGAAAAGTGGTAT
  ATTCCAGACCCAACAGGAAAATTCAGTTTAATCCGAAGACATATGGATACCTCAAACATAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217287 representing NM_029626
  Red=Cloning site Green=Tags(s)
  
  MSFRKVNIIIWVLAVVLFLLVLHHNFLSLSSLLKNDISDSGIVGLQPIDFVASAHQHPVSERQEEIPVVI
  AASEDRLGGTIAAINSVHQNTRSNVMFYIVTFNSTADHLRSWLNSGSLKSIRYKIVNFDTKLLEGKVKQD
  PDQGESMKPLTFARFYLPILVPSAKKAIYMDDDVIVQGDILALYNTPLKPGHAAAFSEDCDSASTKVIIR
  GAGNQYNYIGYLDYKKERIRKLSMKASTCSFNPGVFVANLTEWKRQNVTNQLEKWMKLNVEEGLYSRTLA
  GSITTPPLLIVFYQQHSTIDPMWNVRHLGSSAGKRYSPQFVKAAKLLHWNGHFKPWGRTASYADVWEKWY
  IPDPTGKFSLIRRHMDTSNIK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029626
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029626.2, NP_083902.2
• RefSeq Size: 2485 bp
• RefSeq ORF: 1116 bp
• Locus ID: 76485
• UniProt ID: Q6NSU3
• Cytogenetics: 14 B
• Gene Summary: This gene encodes a member of the glycosyltransferase family. The encoded protein is a type II transmembrane protein containing a glycosyltransferase 8 domain in the lumenal (C-terminal) portion. The specific function of this protein has not been determined. Two alternatively spliced variants encoding the same isoform have been described. [provided by RefSeq, Sep 2009]