Stk31 (NM_029916) Mouse Tagged ORF Clone

CAT#: MG217202

Stk31 (tGFP-tagged) - Mouse serine threonine kinase 31 (Stk31), (10ug)

Product Images

Circular map for MG217202

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Stk31
• Synonyms: C330007K24Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217202 representing NM_029916
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGCCAGCGGCTCTTTGCTGGGACGGCTGTGGCGCAAAGTGTAAGTTTTCCAGGACTTGTCCAAA
  TGGATGAAGATACACATTACAATAAAGTTGAAGATGTTGTTGGAAGTCATGTAGAAGATGCAGTAACATT
  TTGGGCCCAGAATGTCAGTAAAAATAAGGATATTATGAAGATTGGTTGTTCACTCTCAGAAGTTTGTCCT
  CTTGCTAATTCAGTTTTTGGCAATCTTGATCCTAAGAAGATTTATGGTGGATTGTTTTCTGAAGATAAGT
  GCTGGTACAGATGCAAAGTGCTAAAAACTATCAGCGATGATAAGTGCCTGGTGAGGTACATTGACTATGG
  AAATACTGAAATTCTAAACCGATCTGATATAGTAGAAATTCCTCCGGAGCTACAATTTTCTAGTATTGCC
  AAGAAGTATAGACTTTGGGGACTACAGATTCCTTCTGGCCAAGAAGTTACCCAGTTTGATCAGGGTAGAA
  CATTTTTGGGGAGTTTGATTTTTGAAAAAGAAATTAAAATGAGAATAAAAGCAACATATCAAGATGGAAC
  AGTTATTGCTCAGGCTGAGTATGGCACTGTCGATATAGGGGAAGAAGTGGCAAAGAAAGGATTTGCAGAA
  AAGTGCAGACTGACCTCAGGCATTGATGCCTGTGAGGCAAAGAAACCTGATCCTAATCAGCTTGCTCTCA
  GGAGTCTCAAGAACCCTATCCCCCTGTGGGGGCGCAGATCAAACCAGTCAACCTTCAGCAGGCCAAAGGG
  GCATTTTAACGGGAGGCTGACTCTTGATGTGAAGTATGAGACCAGTGCAGGCAATCACGTGACATTTCCA
  AAGGAAAGTTTGGCTGCTGGTGACTTTAATTTAGGGTCTAATGTCAGCTTGGCAAAAATTAAACAGGACC
  AGAAACTTATTGAAGAGAATGAAAAGCTTAAAACAGAGAAAGAGGTTCTTCTAGAAAATTACAAAGCATT
  AGAATTAAAAGTTGAGCAGACTGCCCAGGAGCTGCAGCAAGAGAAAACAGCTACCATGGATCTGACTAAG
  CATTTAGAAAGCACTCTGAAGACGTGTGTAGGAACCAGGCTGAAGAATTTGGCAGCTAAAGTAGAACTAT
  TGAAAGAAATTAGGCATATTAACATCAGTATTCGCTTTGGAAATGACCTTTCAGATGCTATGCAAGTGTT
  GGATGAAGGGTCCTTTACTACTCTAGCATCTTTGAATGAGTTAGAGAAAATTTGGGCTGAATATAATGTT
  GCTCAGGAGAAGATCCAAACTTGTCTTAATGAGAATGAAGGTAATATTTTGATTGCTGAAAGAAATGAAG
  TACAACAGAAGCTGTTCGTGGCTGTAGATGTTTTTATTCTGGAAGTAGATGACTTACCACTTGATAAACG
  CTTAAAAACATTGCAGGACTTAGCAACTTCTTTAGAATCAGTGTATGGAAAGGCCAAAGAAGGAACTAAT
  AACTCTGAAGAAACACTTAGAAAGTTTTTTGACTGGCAGTGTACCAAAAGAGAAGAGTTCGCCAGTATTA
  GGAGTGAAACAGAGGCATCTCTGCAGCACCTTGTGGCATGGTTCCAGAGCAGCCAGAAGGTTTTTGATCT
  GTCCTTGGATGAACCATTGACTTCAGAAGACCTGATTGGTAATATTGACGAAATTCTAGAGAAGACTGAG
  TCATGTGTCTGCAAAGAGCTAGAGCTGTCTCTCATTGAGCAAGGTGTCATAGACAAGGAGATTATTTTAA
  TTACATACAGTCAAGTGCTGCAAAAGATCCATTCTGAGGAAAAGTTCATTGCCACCTTGCTGTCCAAGTA
  TAAGGACAGTGTTGAGTTTAAAAAGCAGATGATTGACTGTTTAAATAAGAACCCCAATGTGGATTACTTG
  CTTTCTATTAAGAAGACATTGAAAGGCTTAAAAGCACAACTGAGATGGAAATTGGTTGAGAAGAGTAATT
  TGGAAGAATCTGATGACCATGATGGAACTGAAATTGAGAAAATAAAGCAAGAAATAACTCAATTGCGAAA
  TAGTGTTTTCCAGGAAATTTATCATGAGAGGGAGGAATATGAGAAGCTGAATAGCTTGACCCAGAAATGG
  TTCCCTGAGCTGCCTCTGCTGTATCCTGAAATAGGATTGCTTAAATATATGAATTCTGGTGGTCTTCTTA
  CTATGAGCTTAGAGCGGGACCTTCTTGACACTGAGCCCATGAAGGAACTTAGCAGCAAGCGTCCTCTGGT
  GTGCTCCGAGGTTAATGGGCAGCCAGTTCTCTTAAAGGGCTATTCCGTGGATGTTGACACAGAAGGCAGG
  GTGATTCAGAGAGCAGCCTCTTACCATAGAGCTTGTGGATATGCTAAAGAAGAGTCTGGGTTACTGCCAT
  TAATATTCTTGTTTTTGTGTAAGTCTGATCCTGTTGCCTATCTGATGGTCCCATATTATCCTAAGGCAAA
  CCTGAGTGCAGTTCAAGCCAGTATGCCTTTAACTTCAGAAGAAGCTTTAAAAGTCATGAAAGGTGTTGCC
  CGAGGACTGCATACATTGCATAGTGCTAACATAATTCATGGATCACTTCATCAGAACAATGTATTTGCCT
  TAAATCGTGAACAAGGGATTGTTGGAGATTATGACTTCACCAAATCTGAGAGCCAGCGAGCTTCAGTCAA
  CGCGATGGTTGGTGGATTGAGTTTGCTCTCACCTGAATTGAAAACTGGAAAACCTCCTTCTGCAAGTTCA
  GACTTATATGCTTATGGTTGCCTTTTCTTATGGCTTTCTGTTCAAAATCAAGAGTTTGAGACAAATGAAG
  ATGGAATTCCAAAAGTAGATCAGTTTCATTTGGATGATAATGTCAAGTCCCTCCTTTGTAGCTTGATATA
  TTTTAGAAGTTCAATGACTGCTGAGCAGGTTTTGAATGCTGAATGTTTCTTGCTTCCAAAGGGGAAATCA
  GTTCCAATCCCAGAAAAAGAGATTGAATGTACTCAGCATAGCAGAGAAGATGAATCAAAGATGGAGAGTC
  TGGATAGATATAGTGAAAAGACAAGAAATGGTGAAGCCAACCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217202 representing NM_029916
  Red=Cloning site Green=Tags(s)
  
  MWGQRLFAGTAVAQSVSFPGLVQMDEDTHYNKVEDVVGSHVEDAVTFWAQNVSKNKDIMKIGCSLSEVCP
  LANSVFGNLDPKKIYGGLFSEDKCWYRCKVLKTISDDKCLVRYIDYGNTEILNRSDIVEIPPELQFSSIA
  KKYRLWGLQIPSGQEVTQFDQGRTFLGSLIFEKEIKMRIKATYQDGTVIAQAEYGTVDIGEEVAKKGFAE
  KCRLTSGIDACEAKKPDPNQLALRSLKNPIPLWGRRSNQSTFSRPKGHFNGRLTLDVKYETSAGNHVTFP
  KESLAAGDFNLGSNVSLAKIKQDQKLIEENEKLKTEKEVLLENYKALELKVEQTAQELQQEKTATMDLTK
  HLESTLKTCVGTRLKNLAAKVELLKEIRHINISIRFGNDLSDAMQVLDEGSFTTLASLNELEKIWAEYNV
  AQEKIQTCLNENEGNILIAERNEVQQKLFVAVDVFILEVDDLPLDKRLKTLQDLATSLESVYGKAKEGTN
  NSEETLRKFFDWQCTKREEFASIRSETEASLQHLVAWFQSSQKVFDLSLDEPLTSEDLIGNIDEILEKTE
  SCVCKELELSLIEQGVIDKEIILITYSQVLQKIHSEEKFIATLLSKYKDSVEFKKQMIDCLNKNPNVDYL
  LSIKKTLKGLKAQLRWKLVEKSNLEESDDHDGTEIEKIKQEITQLRNSVFQEIYHEREEYEKLNSLTQKW
  FPELPLLYPEIGLLKYMNSGGLLTMSLERDLLDTEPMKELSSKRPLVCSEVNGQPVLLKGYSVDVDTEGR
  VIQRAASYHRACGYAKEESGLLPLIFLFLCKSDPVAYLMVPYYPKANLSAVQASMPLTSEEALKVMKGVA
  RGLHTLHSANIIHGSLHQNNVFALNREQGIVGDYDFTKSESQRASVNAMVGGLSLLSPELKTGKPPSASS
  DLYAYGCLFLWLSVQNQEFETNEDGIPKVDQFHLDDNVKSLLCSLIYFRSSMTAEQVLNAECFLLPKGKS
  VPIPEKEIECTQHSREDESKMESLDRYSEKTRNGEANP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029916
• ORF Size: 3054 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029916.1
• RefSeq Size: 3238 bp
• RefSeq ORF: 3057 bp
• Locus ID: 77485
• UniProt ID: Q99MW1
• Cytogenetics: 6 B2.3