Smpd4 (NM_001164611) Mouse Tagged ORF Clone

CAT#: MG216855

Smpd4 (tGFP-tagged) - Mouse sphingomyelin phosphodiesterase 4 (Smpd4) transcript variant 2, (10ug)

Product Images

Circular map for MG216855

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Smpd4
• Synonyms: 4122402O22Rik; mKIAA1418
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216855 representing NM_001164611
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCCCTCACCTACCACAGCCTAGTTTCCTTCTGGCTAGCCTGAAAGCTGACTCTATAAATAAGC
  CCTTTGCACAACGTTGCCAAGATTTGGTGAAAGTCATTGAGGATTTTCCAGCCAAGGAATTGCATGCCGT
  CTTCCCATGGCTGGTGGAAAGCATCTTTGGCAGCCTGGATGGTGTCCTTGTTGGTTGGAATCTCCGATGC
  CTACAGGGACGTGTGAACCCTGTGGAGTACAGCACAGCGATGGAATTTCTAGACCCAAGTGGTCCAATGA
  TGAAGTTGGTTTATAAACTTCAAGCTGAAGACTATAACTTTGATTTTCCTGTCTCCTGTCTTCCTGGCCC
  TGTGAAGGCATCCATTCAAGAGAATGTCCTCCCTGACAGTCCTCTGTACCACAACAAAGTCCAGTTTCCC
  CCGACCGGAGGCCTTGGCCTGAACCTGGCCCTCAATCCATTTGAATACTATATGTTCTACTTTGCTTTGA
  GCCTCATCTCTCAAAAGCCAATGTCTATGACCCTCCATGTCCGTACTTCGGACTGTGCCTATTTCACCCT
  GGTGGATAGGTACCTATCATGGGTCCTACCCACTGAAGGCAGCGTACCTCCACCACTCTGCTCCAGTCCA
  GGAGGCTCCAGCCCCTCACCAGCTCCCAGAACACCAGCCATGCCCTTTGCCTCCTATGGCCTCCACACTA
  GCCTCTTGAAGCGACACATCTCTCATCAGACATCTGTGAATGCAGACCCTGCATCGCATGAGATCTGGCG
  GTCAGAAACTCTCCTCCAGGTTTTTGTGGAAATGTGGCTTCATCATTACTCCCTGGAGATGTACCAAAAA
  ATGCAGTCCCCTCATGCCAAGGAGTCCTTCACGCCTACTGAAGAGCACGTGCTGGTGGTACGCCTGCTGC
  TGAAGCACCTGCATGCCTTTGCCAACAGCCTGAAGCCAGATCAGGCTTCGCCATCTGCACACTCCCATGC
  CACCAGTCCGCTGGAGGAATTCAAACGGGCCGCAGTGCCTCGCTTTGTCCAGCAGAAGCTGTACGTCTTC
  CTGCAGCACTGTTTTGGCCACTGGCCTCTGGATGCAACCTTCAGAGCTGTCTTGGAGATGTGGCTGAGCT
  ACCTGCAGCCATGGAGGTATGCCCCTGAGAAACAGGCTCAGGGCAGCGATCCCCAGCCACGGTGTGTGTC
  AGAGAAGTGGGCACCCTTCATCCAGGAAAACCTGCTGATGTACACCAAGCTGTTTGTGAGCTTTCTGAAC
  CGTGCCCTACGCACAGACCTTGTCAGCCCCAAGAATGCACTCATGGTCTTCCGAGTGGCTAAGGTCTTTG
  CCCAGCCCAACCTGGCTGAGATGATCCAGAAAGGTGAGCAGCTGTTCCTGGAGCCGGAGCTCATCATCCC
  CCACCGCCAACACCGGCTCTTCACAGTCACCACCAGCTTCCTGTCACCATGGCCCCCTGTTGTGACAGAT
  GCCTCCTTCAAGGTGAAGAGCCATGTCTATAGCCTAGAGGGCCAAGACTGCAAATATACCCCAATGTTTG
  GTCCTGAAATCCGAACCCTGGTCTTGCGCCTTGCTCAGCTCATCACACAGGCCAAGCAGACTGCCAAGTC
  CATCTCTGATCAGTATGTGGAAAGCCCAACTGGCCGCTCCTTCCTGTCATGGCTGACCTTTGGCCTCACA
  GACACAAATAGCTGCTACCCAGCCAATGACCTGGATGAAATAGGTCAGGACAGCATCCGCAAGACAGACG
  AGTACTTAGAAAAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCCCAGCTGGCCCAGCT
  CACGCTTGCCCTGGGGAGTGCTCGGGACGAGAATGGGAAGCAGCAGCTCCCAGATTGCATTGTGGGGGAA
  GAAGGACTCATCCTTACACCCTTGGGCCGCTACCAGATCATTAATGGTCTGCGAAGGTTCGAGATTGAGT
  ACCAGGGAGACTTAGAGCTGCAGCCCATCCGGAGCTACGAAATCACCAGTCTGGTCCGTGCACTCTTCCG
  GCTGTCCTCTGCCATCAACCGTAGATTTGCAGGCCAGATGGCAGCTCTGTGCTCCCGGAATGACTTCCTT
  GGCAGCTTCTGTCGCTACCACCTCACTGAGCCTGCCTTGAGCAACAGACATCTGCTGAGCCCTGTAGGGC
  GGAGGCAGGTCACCAATCCTGCACGGGGCCCCAGGCTCAGCCTCCGTTTCCTGGGCAGTTACCGGACACT
  GCTCCTGCTCCTGATGGCCTTCTTTGTGGCTTCTCTGTTCTGCATTGGGCCCCTGTCCTGCTCCTTGCTC
  CTGGTCCTAGGATACGTCCTCTATGCCATAGCTATGACACTGCTCACTGAACGGGGGAAGCTGCACCAGC
  TC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216855 representing NM_001164611
  Red=Cloning site Green=Tags(s)
  
  MAFPHLPQPSFLLASLKADSINKPFAQRCQDLVKVIEDFPAKELHAVFPWLVESIFGSLDGVLVGWNLRC
  LQGRVNPVEYSTAMEFLDPSGPMMKLVYKLQAEDYNFDFPVSCLPGPVKASIQENVLPDSPLYHNKVQFP
  PTGGLGLNLALNPFEYYMFYFALSLISQKPMSMTLHVRTSDCAYFTLVDRYLSWVLPTEGSVPPPLCSSP
  GGSSPSPAPRTPAMPFASYGLHTSLLKRHISHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQK
  MQSPHAKESFTPTEEHVLVVRLLLKHLHAFANSLKPDQASPSAHSHATSPLEEFKRAAVPRFVQQKLYVF
  LQHCFGHWPLDATFRAVLEMWLSYLQPWRYAPEKQAQGSDPQPRCVSEKWAPFIQENLLMYTKLFVSFLN
  RALRTDLVSPKNALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELIIPHRQHRLFTVTTSFLSPWPPVVTD
  ASFKVKSHVYSLEGQDCKYTPMFGPEIRTLVLRLAQLITQAKQTAKSISDQYVESPTGRSFLSWLTFGLT
  DTNSCYPANDLDEIGQDSIRKTDEYLEKALEYLRQIFRLSEAQLAQLTLALGSARDENGKQQLPDCIVGE
  EGLILTPLGRYQIINGLRRFEIEYQGDLELQPIRSYEITSLVRALFRLSSAINRRFAGQMAALCSRNDFL
  GSFCRYHLTEPALSNRHLLSPVGRRQVTNPARGPRLSLRFLGSYRTLLLLLMAFFVASLFCIGPLSCSLL
  LVLGYVLYAIAMTLLTERGKLHQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164611
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164611.1, NP_001158083.1
• RefSeq Size: 4542 bp
• RefSeq ORF: 2382 bp
• Locus ID: 77626
• UniProt ID: Q6ZPR5
• Cytogenetics: 16 A3
• Gene Summary: Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. May sensitize cells to DNA damage-induced apoptosis.[UniProtKB/Swiss-Prot Function]