Smpd4 (NM_001164611) Mouse Tagged ORF Clone
CAT#: MG216855
- TrueORF®
Smpd4 (tGFP-tagged) - Mouse sphingomyelin phosphodiesterase 4 (Smpd4) transcript variant 2, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001164611" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Smpd4 |
Synonyms | 4122402O22Rik; mKIAA1418 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG216855 representing NM_001164611
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCCCTCACCTACCACAGCCTAGTTTCCTTCTGGCTAGCCTGAAAGCTGACTCTATAAATAAGC CCTTTGCACAACGTTGCCAAGATTTGGTGAAAGTCATTGAGGATTTTCCAGCCAAGGAATTGCATGCCGT CTTCCCATGGCTGGTGGAAAGCATCTTTGGCAGCCTGGATGGTGTCCTTGTTGGTTGGAATCTCCGATGC CTACAGGGACGTGTGAACCCTGTGGAGTACAGCACAGCGATGGAATTTCTAGACCCAAGTGGTCCAATGA TGAAGTTGGTTTATAAACTTCAAGCTGAAGACTATAACTTTGATTTTCCTGTCTCCTGTCTTCCTGGCCC TGTGAAGGCATCCATTCAAGAGAATGTCCTCCCTGACAGTCCTCTGTACCACAACAAAGTCCAGTTTCCC CCGACCGGAGGCCTTGGCCTGAACCTGGCCCTCAATCCATTTGAATACTATATGTTCTACTTTGCTTTGA GCCTCATCTCTCAAAAGCCAATGTCTATGACCCTCCATGTCCGTACTTCGGACTGTGCCTATTTCACCCT GGTGGATAGGTACCTATCATGGGTCCTACCCACTGAAGGCAGCGTACCTCCACCACTCTGCTCCAGTCCA GGAGGCTCCAGCCCCTCACCAGCTCCCAGAACACCAGCCATGCCCTTTGCCTCCTATGGCCTCCACACTA GCCTCTTGAAGCGACACATCTCTCATCAGACATCTGTGAATGCAGACCCTGCATCGCATGAGATCTGGCG GTCAGAAACTCTCCTCCAGGTTTTTGTGGAAATGTGGCTTCATCATTACTCCCTGGAGATGTACCAAAAA ATGCAGTCCCCTCATGCCAAGGAGTCCTTCACGCCTACTGAAGAGCACGTGCTGGTGGTACGCCTGCTGC TGAAGCACCTGCATGCCTTTGCCAACAGCCTGAAGCCAGATCAGGCTTCGCCATCTGCACACTCCCATGC CACCAGTCCGCTGGAGGAATTCAAACGGGCCGCAGTGCCTCGCTTTGTCCAGCAGAAGCTGTACGTCTTC CTGCAGCACTGTTTTGGCCACTGGCCTCTGGATGCAACCTTCAGAGCTGTCTTGGAGATGTGGCTGAGCT ACCTGCAGCCATGGAGGTATGCCCCTGAGAAACAGGCTCAGGGCAGCGATCCCCAGCCACGGTGTGTGTC AGAGAAGTGGGCACCCTTCATCCAGGAAAACCTGCTGATGTACACCAAGCTGTTTGTGAGCTTTCTGAAC CGTGCCCTACGCACAGACCTTGTCAGCCCCAAGAATGCACTCATGGTCTTCCGAGTGGCTAAGGTCTTTG CCCAGCCCAACCTGGCTGAGATGATCCAGAAAGGTGAGCAGCTGTTCCTGGAGCCGGAGCTCATCATCCC CCACCGCCAACACCGGCTCTTCACAGTCACCACCAGCTTCCTGTCACCATGGCCCCCTGTTGTGACAGAT GCCTCCTTCAAGGTGAAGAGCCATGTCTATAGCCTAGAGGGCCAAGACTGCAAATATACCCCAATGTTTG GTCCTGAAATCCGAACCCTGGTCTTGCGCCTTGCTCAGCTCATCACACAGGCCAAGCAGACTGCCAAGTC CATCTCTGATCAGTATGTGGAAAGCCCAACTGGCCGCTCCTTCCTGTCATGGCTGACCTTTGGCCTCACA GACACAAATAGCTGCTACCCAGCCAATGACCTGGATGAAATAGGTCAGGACAGCATCCGCAAGACAGACG AGTACTTAGAAAAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCCCAGCTGGCCCAGCT CACGCTTGCCCTGGGGAGTGCTCGGGACGAGAATGGGAAGCAGCAGCTCCCAGATTGCATTGTGGGGGAA GAAGGACTCATCCTTACACCCTTGGGCCGCTACCAGATCATTAATGGTCTGCGAAGGTTCGAGATTGAGT ACCAGGGAGACTTAGAGCTGCAGCCCATCCGGAGCTACGAAATCACCAGTCTGGTCCGTGCACTCTTCCG GCTGTCCTCTGCCATCAACCGTAGATTTGCAGGCCAGATGGCAGCTCTGTGCTCCCGGAATGACTTCCTT GGCAGCTTCTGTCGCTACCACCTCACTGAGCCTGCCTTGAGCAACAGACATCTGCTGAGCCCTGTAGGGC GGAGGCAGGTCACCAATCCTGCACGGGGCCCCAGGCTCAGCCTCCGTTTCCTGGGCAGTTACCGGACACT GCTCCTGCTCCTGATGGCCTTCTTTGTGGCTTCTCTGTTCTGCATTGGGCCCCTGTCCTGCTCCTTGCTC CTGGTCCTAGGATACGTCCTCTATGCCATAGCTATGACACTGCTCACTGAACGGGGGAAGCTGCACCAGC TC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216855 representing NM_001164611
Red=Cloning site Green=Tags(s) MAFPHLPQPSFLLASLKADSINKPFAQRCQDLVKVIEDFPAKELHAVFPWLVESIFGSLDGVLVGWNLRC LQGRVNPVEYSTAMEFLDPSGPMMKLVYKLQAEDYNFDFPVSCLPGPVKASIQENVLPDSPLYHNKVQFP PTGGLGLNLALNPFEYYMFYFALSLISQKPMSMTLHVRTSDCAYFTLVDRYLSWVLPTEGSVPPPLCSSP GGSSPSPAPRTPAMPFASYGLHTSLLKRHISHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQK MQSPHAKESFTPTEEHVLVVRLLLKHLHAFANSLKPDQASPSAHSHATSPLEEFKRAAVPRFVQQKLYVF LQHCFGHWPLDATFRAVLEMWLSYLQPWRYAPEKQAQGSDPQPRCVSEKWAPFIQENLLMYTKLFVSFLN RALRTDLVSPKNALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELIIPHRQHRLFTVTTSFLSPWPPVVTD ASFKVKSHVYSLEGQDCKYTPMFGPEIRTLVLRLAQLITQAKQTAKSISDQYVESPTGRSFLSWLTFGLT DTNSCYPANDLDEIGQDSIRKTDEYLEKALEYLRQIFRLSEAQLAQLTLALGSARDENGKQQLPDCIVGE EGLILTPLGRYQIINGLRRFEIEYQGDLELQPIRSYEITSLVRALFRLSSAINRRFAGQMAALCSRNDFL GSFCRYHLTEPALSNRHLLSPVGRRQVTNPARGPRLSLRFLGSYRTLLLLLMAFFVASLFCIGPLSCSLL LVLGYVLYAIAMTLLTERGKLHQL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001164611 |
ORF Size | 2379 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164611.1, NP_001158083.1 |
RefSeq Size | 4542 bp |
RefSeq ORF | 2382 bp |
Locus ID | 77626 |
UniProt ID | Q6ZPR5 |
Cytogenetics | 16 A3 |
Gene Summary | Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. May sensitize cells to DNA damage-induced apoptosis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221644 | Smpd4 (untagged) - Mouse sphingomyelin phosphodiesterase 4 (Smpd4), transcript variant 2, (10ug) |
USD 800.00 |
|
MR216855 | Smpd4 (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase 4 (Smpd4), transcript variant 2 |
USD 799.00 |
|
MR216855L3 | Lenti ORF clone of Smpd4 (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase 4 (Smpd4), transcript variant 2 |
USD 1,099.00 |
|
MR216855L4 | Lenti ORF clone of Smpd4 (mGFP-tagged) - Mouse sphingomyelin phosphodiesterase 4 (Smpd4), transcript variant 2 |
USD 1,099.00 |
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