Scg3 (NM_009130) Mouse Tagged ORF Clone
CAT#: MG215897
- TrueORF®
Scg3 (tGFP-tagged) - Mouse secretogranin III (Scg3) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_009130" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Scg3 |
Synonyms | 1B1075; AI385542; Chgd; SgIII |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG215897 representing NM_009130
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTTCCTTTGGACCGGCTCTTGGATACTGGTGTTGGTGCTCAACAGCGGCCCAATTCAAGCTTTCC CCAAACCCGAAGGCAGCCAAGACAAATCCCTGCATAATAGAGAATTAAGTGCAGAAAGACCTTTGAATGA ACAGATCGCTGAGGCAGAGGCAGACAAGATTAAAAAGGCATTCCCTTCAGAAAGCAAGCCGAGTGAAAGC AATTATTCTTCTGTCGATAACTTGAATCTGCTGAGGGCAATAACAGAAAAGGAAACCGTTGAGAAAGAGA GACAATCCATAAGAAGCCCCCCGTTTGATAACCAACTGAACGTGGAAGACGCTGATTCAACCAAAAATCG GAAACTGATCGATGAGTACGATTCCACCAAGAGTGGACTGGACCACAAGTTTCAAGATGACCCAGACGGC CTTCATCAACTGGATGGAACTCCTTTAACTGCTGAAGACATCGTCCATAAGATTGCCACCAGGATTTATG AGGAGAACGACAGAGGAGTGTTTGACAAAATTGTTTCTAAACTGCTGAATCTTGGCCTGATCACTGAAAG CCAGGCACATACTCTGGAAGATGAAGTAGCAGAAGCTTTACAAAAACTGATTTCAAAAGAGGCCAACAAT TATGAGGAGACCCTGGATAAACCCACAAGCAGGACCGAGAATCAGGATGGGAAAATACCAGAGAAAGTGA CTCCGGTGGCAGCAGTCCAAGATGGCTTCACTAACCGTGAAAACGATGAGACGGTGTCTAACACCTTGAC CTTGTCCAATGGCTTGGAAAGGAGAACTAACCCCCACAGGGAAGACGACTTTGAGGAACTCCAGTATTTC CCCAACTTCTATGCACTACTGACAAGCATCGACTCAGAAAAAGAAGCAAAAGAGAAAGAAACCCTGATCA CCATCATGAAGACATTGATTGACTTCGTGAAAATGATGGTGAAATACGGTACGATATCTCCAGAGGAAGG CGTGTCCTACCTTGAAAACTTGGATGAAACAATTGCTCTGCAGACCAAGAACAAGCTAGAAAAAAATACT ACTGATAGCAAAAGTAAGCTATTCCCAGCTCCACCAGAGAAGAGTCAGGAAGAAACAGACAGTACCAAGG AAGAAGCCGCCAAGATGGAAAAGGAATACGGAAGCCTAAAAGACTCTACAAAAGATGATAACTCCAACCT AGGAGGAAAGACAGATGAAGCCACAGGGAAGACAGAAGCCTACTTGGAAGCCATTAGAAAAAACATCGAA TGGCTGAAGAAACATAACAAGAAGGGCAACAAAGAAGATTACGACCTTTCAAAGATGAGGGACTTTATCA ACCAACAAGCTGACGCTTATGTGGAGAAGGGCATCCTCGACAAGGAAGAAGCCAACGCCATCAAACGCAT CTACAGCAGCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215897 representing NM_009130
Red=Cloning site Green=Tags(s) MGFLWTGSWILVLVLNSGPIQAFPKPEGSQDKSLHNRELSAERPLNEQIAEAEADKIKKAFPSESKPSES NYSSVDNLNLLRAITEKETVEKERQSIRSPPFDNQLNVEDADSTKNRKLIDEYDSTKSGLDHKFQDDPDG LHQLDGTPLTAEDIVHKIATRIYEENDRGVFDKIVSKLLNLGLITESQAHTLEDEVAEALQKLISKEANN YEETLDKPTSRTENQDGKIPEKVTPVAAVQDGFTNRENDETVSNTLTLSNGLERRTNPHREDDFEELQYF PNFYALLTSIDSEKEAKEKETLITIMKTLIDFVKMMVKYGTISPEEGVSYLENLDETIALQTKNKLEKNT TDSKSKLFPAPPEKSQEETDSTKEEAAKMEKEYGSLKDSTKDDNSNLGGKTDEATGKTEAYLEAIRKNIE WLKKHNKKGNKEDYDLSKMRDFINQQADAYVEKGILDKEEANAIKRIYSSL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009130 |
ORF Size | 1413 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009130.3, NP_033156.1 |
RefSeq Size | 2207 bp |
RefSeq ORF | 1416 bp |
Locus ID | 20255 |
UniProt ID | P47867 |
Cytogenetics | 9 42.32 cM |
Gene Summary | Member of the granin protein family that regulates the biogenesis of secretory granules (PubMed:16219686). Acts as a sorting receptor for intragranular proteins including chromogranin A/CHGA (PubMed:12388744). May also play a role in angiogenesis (PubMed:28330905). Promotes endothelial proliferation, migration and tube formation through MEK/ERK signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216479 | Scg3 (untagged) - Mouse secretogranin III (Scg3), transcript variant 1, (10ug) |
USD 503.00 |
|
MR215897 | Scg3 (Myc-DDK-tagged) - Mouse secretogranin III (Scg3), transcript variant 1 |
USD 503.00 |
|
MR215897L3 | Lenti ORF clone of Scg3 (Myc-DDK-tagged) - Mouse secretogranin III (Scg3), transcript variant 1 |
USD 803.00 |
|
MR215897L4 | Lenti ORF clone of Scg3 (mGFP-tagged) - Mouse secretogranin III (Scg3), transcript variant 1 |
USD 803.00 |
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