Adcy8 (NM_009623) Mouse Tagged ORF Clone

CAT#: MG215716

Adcy8 (tGFP-tagged) - Mouse adenylate cyclase 8 (Adcy8), (10ug)

Product Images

Circular map for MG215716

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adcy8
• Synonyms: AC8; AW060868
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215716 representing NM_009623
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCTCGGATGTGCACTGCCTTAGTGGCAGCGAGGAACTCTACACCATCCAACCGACGCCCCCGG
  CCGGCGACGACGGGAGCGGCTCTCGGCCGCAGCGGCTGCTGTGGCAGACGGCGGTGCGGCACATCACGGA
  GCAGCGCTTCATCCACGGGCACCGAGGCGGCGGCGGCGGCGGGGTCTCCCGCAAAGCCTCGAACCCTGCG
  GGCAGTGGACCCAATCACCACGCGCCGCAGCTGTCTAGCGACTCGGTGCTGCCTCTCTATTCTCTGGGCC
  CCGGAGAGCGAGCGCACAACACCGGTGGCACCAAAGTCTTTCCGGAACGCAGCGGGAGCGGCAGTGCCAG
  TGGCAGCGGGGGCGGGGGCGACTTGGGCTTCCTACACCTTGACTGTGCCCCAAGTAACTCGGATTTCTTC
  CTCAATGGAGGATACAGCTACCGAGGGGTCATTTTTCCAACCCTACGCAACTCCTTCAAGTCTCGGGATC
  TGGAGCGCCTCTACCAACGCTATTTCCTGGGCCAGAGGCGCAAATCGGAGGTAGTGATGAACGTGTTGGA
  TGTACTAACCAAACTCACCCTTTTAGTCCTGCACTTGAGCCTGGCCTCGGCTCCAATGGACCCTCTCAAG
  GGCATCCTGCTAGGCTTTTTCACTGGCATCGAGGTGGTGATCTGCGCCCTCGTGGTGGTCAGGAAGGACA
  ACACCTCCCACACTTACCTGCAATACAGCGGCGTGGTCACTTGGGTGGCTATGACCACCCAGATTCTGGC
  AGCAGGCCTGGGCTATGGGCTTCTGGGCGACGGCATAGGCTACGTGCTTTTTACACTCTTCGCCACCTAC
  AGCATGCTTCCCCTGCCTCTCACCTGGGCCATCTTGGCCGGCCTGGGCACATCTTTGCTGCAAGTCACCC
  TTCAAGTACTCATACCCAGGCTAGCGGTCTTTTCCATCAACCAGGTCCTGGCCCAGGTGGTGCTATTCAT
  GTGTATGAATACAGCAGGAATCTTCATCAGTTACCTTTCAGACCGTGCCCAGCGCCAGGCCTTCCTGGAG
  ACCCGGAGGTGTGTGGAGGCCAGGCTCCGCTTGGAGACAGAGAACCAAAGACAGGAGCGGCTTGTGCTGT
  CTGTGCTCCCCAGGTTTGTCGTCCTGGAAATGATCAACGACATGACCAATGTGGAGGATGAGCACCTGCA
  GCATCAGTTCCACCGCATCTACATCCATCGCTATGAGAACGTCAGTATTCTTTTTGCAGATGTCAAAGGA
  TTTACCAACCTCTCTACGACCTTGTCTGCTCAGGAGCTGGTCAGGATGCTCAACGAACTCTTTGCCAGAT
  TCGACCGGCTGGCCCATGAGCATCACTGTCTTCGCATTAAAATCCTGGGGGACTGCTACTACTGTGTGTC
  TGGACTGCCTGAGCCCCGCCGGGACCACGCTCATTGCTGTGTTGAAATGGGCCTCAGCATGATCAAAACT
  ATCAGGTTTGTGAGATCCAGAACGAAGCACGATGTTGACATGCGAATTGGAATCCATTCAGGTTCTGTGC
  TATGTGGTGTGTTGGGCCTGAGGAAATGGCAGTTTGATGTCTGGTCTTGGGATGTGGACATAGCAAACAA
  GCTTGAGTCTGGAGGAATCCCTGGGAGGATTCACATTTCTAAAGCCACCCTGGATTGCCTCAATGGTGAC
  TATAACGTGGAAGAGGGTCACGGGAAAGAGAGGAACGAATTCTTGAGGAAGCATAACATAGAGACCTATT
  TGATTAAGCAGCCTGAGGAGAGTTTGCTCTGCTTGCCTGAAGACATCGTTAAGGAGTCGGTGAGCTGCTC
  TGACAGGAGAAACAGTGGAGCAACATTCACAGAAGGATCGTGGAGCCCAGAGCTGCCTTTCGACAACATC
  GTGGGCAAACAGAATACTCTGGCTGCCCTAACAAGAAATTCAATAAATCTGCTTCCAAACCATCTCGCAC
  AAGCTTTGCATGTCCAGTCTGGGCCTGAGGAAATTAACAAGAGAATAGAGCATACCATCGACTTGCGGAG
  TGGCGATAAGTTGAGAAGAGAGCATATCAAGCCATTCTCACTGATGTTTAAAGACTCCAGCCTGGAGCAC
  AAGTATTCTCAAATGAGGGATGAAGTATTCAAGTCAAACTTGGTCTGTGCATTTATCGTTCTTCTGTTTA
  TCACTGCAATTCAAAGTTTGCTTCCTTCTTCAAGGCTGATGCCTATGACTATCCAGTTCTCCATCCTGAT
  CATGCTGCACTCTGCCCTGGTCCTCATCACCACAGCAGAAGACTATAAGTGTCTGCCCCTCATCCTCCGA
  AAAACCTGCTGTTGGATTAATGAGACCTATCTGGCCCGCAACGTCATCATCTTCGCTTCCATCTTAATTA
  ACTTCCTGGGAGCTGTCCTAAATATTCTGTGGTGTGATTTTGACAAGTCGATACCCTTGAAGAACCTGAC
  TTTCAATTCCTCAGCTGTGTTTACAGATATCTGCTCCTACCCAGAGTACTTTGTCTTCACTGGGGTGTTG
  GCCATGGTGACCTGTGCTGTGTTTCTACGGCTTAACTCTGTCCTGAAGCTGGCAGTGCTACTCATCATGA
  TCGCCATCTACGCCCTGCTCACAGAGACCATCTACGCAGGTCTCTTTCTGAGTTATGACAACCTGAACCA
  CAGTGGAGAAGATTTCCTGGGGACCAAGGAAGCATCACTGCTGCTGATGGCCATGTTCCTTCTTGCTGTG
  TTCTACCATGGACAACAGCTGGAGTACACAGCCCGCCTGGATTTCCTGTGGCGCGTACAGGCCAAAGAGG
  AGATCAACGAGATGAAGGAACTGAGGGAACACAACGAGAACATGCTGCGCAATATCTTACCCAGCCATGT
  GGCCCGCCACTTCCTGGAGAAAGACAGAGACAATGAGGAGCTGTATTCTCAATCCTACGATGCTGTTGGA
  GTGATGTTCGCCTCCATCCCTGGGTTCGCAGACTTCTACTCTCAGACAGAAATGAACAACCAGGGAGTAG
  AATGTCTGCGCTTGCTGAATGAGATCATTGCTGACTTTGATGAGCTGCTCGGGGAGGACCGGTTTCAGGA
  CATTGAGAAGATTAAGACCATTGGTAGTACATACATGGCTGTCTCAGGACTGTCCCCAGAGAAACAGCAA
  TGTGAAGATAAATGGGGACATTTGTGTGCCCTGGCTGACTTCTCTCTTGCTCTGACTGAAAGCATACAAG
  AGATCAACAAGCATTCATTCAACAATTTTGAACTCCGAATCGGCATCAGCCATGGCTCAGTGGTGGCAGG
  TGTAATTGGAGCTAAGAAACCACAGTATGACATTTGGGGGAAAACTGTGAACTTGGCAAGCCGAATGGAC
  AGCACGGGAGTAAGTGGCCGGATCCAAGTTCCTGAGGAGACCTACCTTATCCTGAAGGACCAGGGCTTTG
  CCTTTGACTACCGTGGGGAGATATATGTGAAGGGCATCAGTGAACAAGAAGGGAAAATCAAAACATACTT
  TCTCCTGGGACGAGTCCAACCCAACCCATTCATCTTACCCCCAAGGAGACTTCCCGGGCAATACTCTCTG
  GCTGCGGTTGTCCTTGGCCTTGTCCAGTCTCTCAACAGGCAAAGGCAGAAGCAACTTCTCAACGAGAACA
  GCAATTCGGGCATCATCAAGAGCCATTACAACCGGCGGACTTTGCTAACGCCAAGTGGGCCAGAGCCTGG
  AGCACAAGCTGAAGGCACTGACAAATCCGATTTGCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215716 representing NM_009623
  Red=Cloning site Green=Tags(s)
  
  MELSDVHCLSGSEELYTIQPTPPAGDDGSGSRPQRLLWQTAVRHITEQRFIHGHRGGGGGGVSRKASNPA
  GSGPNHHAPQLSSDSVLPLYSLGPGERAHNTGGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSDFF
  LNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLK
  GILLGFFTGIEVVICALVVVRKDNTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATY
  SMLPLPLTWAILAGLGTSLLQVTLQVLIPRLAVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLE
  TRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKG
  FTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRRDHAHCCVEMGLSMIKT
  IRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLNGD
  YNVEEGHGKERNEFLRKHNIETYLIKQPEESLLCLPEDIVKESVSCSDRRNSGATFTEGSWSPELPFDNI
  VGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEH
  KYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILR
  KTCCWINETYLARNVIIFASILINFLGAVLNILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTGVL
  AMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSGEDFLGTKEASLLLMAMFLLAV
  FYHGQQLEYTARLDFLWRVQAKEEINEMKELREHNENMLRNILPSHVARHFLEKDRDNEELYSQSYDAVG
  VMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEKQQ
  CEDKWGHLCALADFSLALTESIQEINKHSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMD
  STGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSL
  AAVVLGLVQSLNRQRQKQLLNENSNSGIIKSHYNRRTLLTPSGPEPGAQAEGTDKSDLP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009623
• ORF Size: 3747 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009623.2, NP_033753.2
• RefSeq Size: 5064 bp
• RefSeq ORF: 3750 bp
• Locus ID: 11514
• UniProt ID: P97490
• Cytogenetics: 15 29.03 cM
• Gene Summary: Catalyzes the formation of cAMP in response to calcium entry leadings to cAMP signaling activation that affect processes suche as synaptic plasticity and insulin secretion (PubMed:10864938, PubMed:25403481, PubMed:10482244, PubMed:14585998, PubMed:18448650). Plays a role in many brain functions, such as learning, memory, drug addiction, and anxiety modulation through regulation of synaptic plasticity by modulating long-term memory and long-term potentiation (LTP) through CREB transcription factor activity modulation (PubMed:10482244, PubMed:14585998, PubMed:18448650, PubMed:10864938, PubMed:12441059, PubMed:20638449, PubMed:27234425, PubMed:18222416). Plays a central role in insulin secretion by controlling glucose homeostasis through glucagon-like peptide 1 and glucose signaling pathway and maintains insulin secretion through calcium-dependent PKA activation leading to vesicle pool replenishment (PubMed:25403481). Also, allows PTGER3 to induce potentiation of PTGER4-mediated PLA2 secretion by switching from a negative to a positive regulation, during the IL1B induced-dedifferentiation of smooth muscle cells (By similarity).[UniProtKB/Swiss-Prot Function]