Adgrb1 (NM_174991) Mouse Tagged ORF Clone

CAT#: MG215473

Bai1 (tGFP-tagged) - Mouse brain-specific angiogenesis inhibitor 1 (Bai1), (10ug)

Product Images

Circular map for MG215473

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adgrb1
• Synonyms: B830018M07Rik; Bai1; mKIAA4089; R75078
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215473 representing NM_174991
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGCCAGGCTGCTGCCCCGGGCCCCATCTGGATCCTTGCTCCACTCCTGCTGTTACTATTGCTAT
  TGGGGCGCTGGGCGCGCGCTGCCTCTGGGGCAGACATAGGGCCTGGGACAGAGCAGTGCACCACGTTGGT
  GCAGGGAAAGTTCTTCGGCTACTTCTCGGCGGCTGCTGTATTTCCGGCCAACGCCTCACGCTGCTCTTGG
  ACCCTTCGCAACCCGGACCCTCGTCGTTACACGCTCTACATGAAGGTGGCCAAGGCACCTGCACCCTGCA
  GCGGCCCCGGCCGAGTTCGTACCTACCAGTTCGACTCTTTCTTGGAGTCTACACGCACCTACCTGGGTGT
  GGAGAGCTTTGACGAAGTTCTGCGGCTCTGCGATTCCTCTGCACCCCTCGCCTTTCTACAGGCCAGCAAA
  CAGTTTCTACAGATGCAACGCCAACAGCCACCCCAAGACGGTGACCTCGGACCCCAGGGCGAGTTCCCCA
  GCTCTAGTGACGACTTCTCCGTGGAGTACCTGGTGGTGGGCAATCGCAACCCCAGCCATGCTGCCTGTCA
  GATGCTGTGTCGCTGGCTGGATGCCTGTCTAGCCGGCAGCCGCAGCTCACACCCCTGTGGCATCATGCAG
  ACTCCCTGCGCCTGCCTGGGTGGAGATGTTGGAGACCCTGCTTCCAGCCCCTTGGTCCCCCGTGGGGACG
  TCTGCTTGAGGGACGGCGTAGCTGGTGGCCCTGAGAACTGCCTCACCAGCCTGACCCAGGACCGGGGTGG
  GCACGGCTCAGCAGGTGGCTGGAAACTGTGGTCCTTGTGGGGCGAATGTACGCGGGACTGCGGGGGAGGC
  CTGCAAACTCGAACCCGCACTTGCTTACCCACCCTTGGTGTGGAGGGCGGAGGCTGCGAGGGCGTTCTGG
  AAGAAGGTCGCCTGTGCAATCGCAAGGCCTGCGGTCCCACTGGGCGCAGCAGCTCACGGAGCCAGTCCCT
  GAGGTCCACGGATGCCCGGCGACGTGAGGAGTTTGGGGATGAGCTGCAGCAGTTTGGGTTCCCATCTCCC
  CAGACTGGTGACCCAGCAGCTGAGGAGTGGTCCCCATGGAGTGTATGCTCCAGTACCTGCGGTGAGGGCT
  GGCAGACCCGCACCCGCTTCTGCGTGTCATCCTCGTACAGTACTCAGTGCAGTGGACCTCTGAGGGAGCA
  GCGGCTCTGCAACAACTCAGCCGTGTGTCCAGTGCATGGAGCCTGGGATGAGTGGTCACCCTGGAGCCTC
  TGCTCCAGCACCTGTGGCCGTGGCTTCCGTGACCGCACTCGCACCTGCAGGCCCCCTCAGTTTGGAGGCA
  ACCCCTGTGAGGGTCCTGAGAAGCAAACCAAGTTCTGCAACATTGCTCTGTGCCCTGGCCGGGCAGTGGA
  TGGAAACTGGAACGAATGGTCCAGCTGGAGCACCTGCTCTGCCAGTTGCTCCCAAGGCCGCCAGCAGCGG
  ACCCGAGAGTGCAATGGTCCTTCCTATGGAGGCGCTGAGTGCCAGGGCCACTGGGTGGAGACTCGAGACT
  GCTTCCTGCAGCAATGCCCAGTGGATGGCAAATGGCAGGCCTGGGCCTCATGGGGCAGCTGCAGCGTCAC
  ATGTGGAGGTGGAAGCCAGCGACGGGAGCGTGTCTGCTCTGGGCCTTTCTTTGGGGGAGCAGCCTGCCAG
  GGGCCCCAGGACGAGTACCGACAGTGTGGCGCTCAACGATGTCCTGAGCCCCATGAGATTTGTGATGAGG
  ACAACTTTGGGGCCGTGGTCTGGAAGGAGACCCCAGCTGGAGAGGTGGCTGCAGTCCGGTGTCCCCGCAA
  TGCCACAGGCCTCATCCTGCGCCGCTGTGAGCTGGACGAGGAGGGCATCGCCTTCTGGGAGCCGCCCACC
  TACATCCGCTGCGTCTCCATCGACTACCGGAACATCCAGATGATGACCCGAGAACACCTGGCAAAGGCTC
  AGCGGGGGCTGCCAGGGGAGGGGGTCTCAGAGGTCATCCAGACACTGCTGGAGATCTCACAGGATGGCAC
  CAGCTACAGCGGCGACCTGCTCTCCACCATTGATGTCCTGAGGAACATGACAGAGATTTTCCGCAGGGCT
  TACTACAGCCCCACGCCAGGGGATGTGCAGAACTTTGTCCAGATCATCAGCAACCTGCTGGCAGAAGAGA
  ACCGGGACAAGTGGGAGGAGGCGCAGCTGATGGGCCCCAATGCCAAGGAGCTCTTTCGGCTGGTGGAGGA
  CTTTGTGGATGTCATCGGCTTCCGCATGAAGGATTTGAGGGATGCCTACCAGGTGACAGACAATCTGGTG
  CTCAGCATCCACAAGCTTCCGGCCAGTGGGGCCACCGACATCAGCTTCCCTATGAAGGGCTGGCGTGCCA
  CCGGAGACTGGGCCAAGGTGCCAGAGGACAGGGTCACCGTGTCCAAGAGTGTGTTTTCCACAGGGCTGGC
  AGAAGCTGACGATTCATCTGTGTTCGTGGTTGGTACTGTGCTCTACCGAAACTTGGGCAGCTTCCTGGCC
  CTGCAGAGGAACACAACTGTCCTCAACTCTAAGGTCATCTCCGTGACTGTGAAGCCCCCGCCTCGATCGC
  TGCTCACACCCTTGGAGATCGAGTTTGCCCACATGTACAACGGCACTACCAACCAGACCTGTATCCTGTG
  GGATGAGACAGATGGACCCTCCTCCTCCGCCCCCCCGCAGCTCGGGCCCTGGTCGTGGCGCGGCTGCCGC
  ACGGTGCCCCTCGATGCCCTCCGGACGCGCTGCCTGTGTGACCGGCTCTCCACCTTCGCCATCTTAGCCC
  AGCTCAGCGCCGACGCGACCATGGATAAGGTGACCGTGCCCTCTGTGACGCTCATCGTGGGCTGTGGCGT
  GTCTTCCCTCACCCTGCTCATGCTGGTCATCATCTACGTGTCTGTGTGGAGGTACATTCGCTCAGAGCGG
  TCCGTTATCCTCATCAATTTCTGCCTGTCCATCATCTCCTCTAATGCTCTCATCCTTATCGGGCAGACCC
  AGACCCGCAACAAGGTTGTGTGCACGCTGGTGGCTGCCTTCTTGCACTTCTTCTTCCTGTCCTCATTTTG
  CTGGGTGCTCACCGAGGCCTGGCAATCCTACATGGCCGTCACCGGCCGCCTCCGGAGCCGGCTCGTCCGC
  AAACGCTTTCTCTGCCTGGGCTGGGGGCTCCCTGCACTGGTGGTGGCCATTTCTGTGGGATTCACCAAGG
  CCAAGGGCTACAGCACCATGAACTATTGCTGGCTCTCCCTGGAGGGGGGACTTCTGTATGCCTTCGTGGG
  ACCGGCAGCTGCAGTTGTGCTGGTGAACATGGTGATCGGGATCCTGGTGTTCAACAAGCTCGTGTCCAAG
  GACGGCATCACGGACAAGAAGCTGAAGGAGCGGGCAGGGGCCTCCCTGTGGAGCTCCTGCGTGGTGCTGC
  CGCTGCTGGCGCTGACCTGGATGTCTGCTGTGCTTGCTGTCACCGACCGCCGCTCCGCCCTCTTCCAGAT
  CCTCTTCGCTGTCTTTGACTCACTGGAGGGCTTCGTCATCGTGATGGTGCACTGCATTCTGCGCAGAGAG
  GTCCAGGATGCTGTGAAGTGCCGTGTGGTAGACCGACAAGAGGAGGGCAACGGGGACTCAGGGGGCTCCT
  TCCAGAACGGCCATGCCCAGCTCATGACCGACTTTGAGAAGGATGTGGATCTGGCCTGTAGATCAGTGCT
  GAACAAAGACATTGCAGCCTGCCGCACAGCAACTATCACAGGTACCTTCAAGCGACCATCACTGCCTGAA
  GAGGAGAAGATGAAGCTGGCCAAGGGCCCACCCCCCACCTTCAACAGCCTGCCAGCTAATGTGTCCAAGC
  TGCACCTGCACGGTTCACCCCGCTATCCTGGTGGGCCACTGCCTGACTTCCCTAATCACTCACTCACCCT
  CAAAAAGGACAAGGCCCCCAAGTCCTCTTTTATTGGCGATGGGGACATCTTTAAGAAACTGGACTCCGAG
  CTGAGCCGAGCCCAGGAGAAGGCTCTGGACACGAGCTACGTGATCCTGCCCACGGCCACGGCCACACTCC
  GGCCCAAACCCAAGGAGGAACCCAAATACAGCATCAACATTGACCAGATGCCCCAAACCCGCCTCATCCA
  CCTTAGCATGGCACCTGATGCCAGCTTCCCTACCCGAAGCCCACCTGCCCGTGAGCCCCCAGGTGGTGCA
  CCCCCTGAGGTGCCCCCTGTCCAGCCCCCACCACCGCCACCTCCACCACCCCCTCCACCCCAACAGCCCA
  TACCCCCACCACCCACCCTGGAGCCTGCACCTCCCAGCCTGGGGGACACGGGGGAGCCTGCGGCCCACCC
  GGGACCCAGTTCAGGTGCTGGGGCCAAGAATGAGAACGTGGCCACCTTGTCAGTGAGCTCTCTGGAGCGG
  CGGAAATCACGGTATGCAGAACTGGACTTTGAGAAGATCATGCACACACGGAAGCGACACCAAGACATGT
  TCCAGGACCTGAACCGGAAGCTGCAGCATGCGGCTGAGAAGGAGAAGGAGGTGCCAGGCGCAGACAGCAA
  GCCAGAGAAGCAGCAGACTCCCAACAAGAGGGCCTGGGAGAGCCTCCGCAAGCCCCACGGGACGCCTGCC
  TGGGTAAAAAAGGAGCTGGAGCCACTGCCCCCGTCTCCACTGGAGCTGCGGAGCGTAGAGTGGGAGAAGG
  CGGGTGCCACCATCCCACTGGTTGGCCAGGACATCATTGACCTCCAGACCGAGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215473 representing NM_174991
  Red=Cloning site Green=Tags(s)
  
  MRGQAAAPGPIWILAPLLLLLLLLGRWARAASGADIGPGTEQCTTLVQGKFFGYFSAAAVFPANASRCSW
  TLRNPDPRRYTLYMKVAKAPAPCSGPGRVRTYQFDSFLESTRTYLGVESFDEVLRLCDSSAPLAFLQASK
  QFLQMQRQQPPQDGDLGPQGEFPSSSDDFSVEYLVVGNRNPSHAACQMLCRWLDACLAGSRSSHPCGIMQ
  TPCACLGGDVGDPASSPLVPRGDVCLRDGVAGGPENCLTSLTQDRGGHGSAGGWKLWSLWGECTRDCGGG
  LQTRTRTCLPTLGVEGGGCEGVLEEGRLCNRKACGPTGRSSSRSQSLRSTDARRREEFGDELQQFGFPSP
  QTGDPAAEEWSPWSVCSSTCGEGWQTRTRFCVSSSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSL
  CSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPGRAVDGNWNEWSSWSTCSASCSQGRQQR
  TRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGGGSQRRERVCSGPFFGGAACQ
  GPQDEYRQCGAQRCPEPHEICDEDNFGAVVWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAFWEPPT
  YIRCVSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLLEISQDGTSYSGDLLSTIDVLRNMTEIFRRA
  YYSPTPGDVQNFVQIISNLLAEENRDKWEEAQLMGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLV
  LSIHKLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLAEADDSSVFVVGTVLYRNLGSFLA
  LQRNTTVLNSKVISVTVKPPPRSLLTPLEIEFAHMYNGTTNQTCILWDETDGPSSSAPPQLGPWSWRGCR
  TVPLDALRTRCLCDRLSTFAILAQLSADATMDKVTVPSVTLIVGCGVSSLTLLMLVIIYVSVWRYIRSER
  SVILINFCLSIISSNALILIGQTQTRNKVVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGRLRSRLVR
  KRFLCLGWGLPALVVAISVGFTKAKGYSTMNYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSK
  DGITDKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRRE
  VQDAVKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSVLNKDIAACRTATITGTFKRPSLPE
  EEKMKLAKGPPPTFNSLPANVSKLHLHGSPRYPGGPLPDFPNHSLTLKKDKAPKSSFIGDGDIFKKLDSE
  LSRAQEKALDTSYVILPTATATLRPKPKEEPKYSINIDQMPQTRLIHLSMAPDASFPTRSPPAREPPGGA
  PPEVPPVQPPPPPPPPPPPPQQPIPPPPTLEPAPPSLGDTGEPAAHPGPSSGAGAKNENVATLSVSSLER
  RKSRYAELDFEKIMHTRKRHQDMFQDLNRKLQHAAEKEKEVPGADSKPEKQQTPNKRAWESLRKPHGTPA
  WVKKELEPLPPSPLELRSVEWEKAGATIPLVGQDIIDLQTEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174991
• ORF Size: 4746 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174991.3, NP_778156.2
• RefSeq Size: 6227 bp
• RefSeq ORF: 4749 bp
• Locus ID: 107831
• UniProt ID: Q3UHD1
• Cytogenetics: 15 D3
• Gene Summary: Phosphatidylserine receptor which enhances the engulfment of apoptotic cells (PubMed:17960134). Also mediates the binding and engulfment of Gram-negative bacteria (PubMed:21245295, PubMed:26838550, PubMed:26838550). Stimulates production of reactive oxygen species by macrophages in response to Gram-negative bacteria, resulting in enhanced microbicidal macrophage activity (By similarity). In the gastric mucosa, required for recognition and engulfment of apoptotic gastric epithelial cells (By similarity). Promotes myoblast fusion (PubMed:23615608). Activates the Rho pathway in a G-protein-dependent manner (By similarity). Inhibits MDM2-mediated ubiquitination and degradation of DLG4/PSD95, promoting DLG4 stability and regulating synaptic plasticity (PubMed:25751059). Required for the formation of dendritic spines by ensuring the correct localization of PARD3 and TIAM1 (By similarity). Potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53/TP53 signal in suppression of glioblastoma (By similarity).[UniProtKB/Swiss-Prot Function]