Tpte (NM_199257) Mouse Tagged ORF Clone

CAT#: MG214933

Tpte (tGFP-tagged) - Mouse transmembrane phosphatase with tensin homology (Tpte), (10ug)

Product Images

Circular map for MG214933

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tpte
• Synonyms: Pten2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214933 representing NM_199257
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGGAGAAAAGAAGAGCCATTTGTATCTCTGGATGGAACACTATGGGTATGACATGCCTGCAAATA
  TTTACAAGATGTATTCTCAGCCCTCCAGAAAAACGGATGATGCAAATAAAAAGGTGTCAGTGAGTGCTTC
  AAGGACCATTAAACTAAATGGCAGCACTGGCTACGACACAAATGAACAGATAACACTAATCACTAACGGG
  AGCAGTTTGTCATATCCAGATGAAATAAAATCAGCAAGTTATGCGGACCCCATCAGTACAAAAGCATATA
  CAAATGATTCGTCAGTATACGACCCTGGGGGAGCTTCGAGCAGCACAACCCTGTATGAACTGAATTCCTT
  GAGTGAAGTATCGAAAGAAATCATTACCCAGGGAGAATCTGCTTTACTACGTGATAAAGAAGCTACCAGC
  GAACTCAAAATCCCAAGTACACTGCAAACGCAGACGTCCATGTCCACCAACACTCTCTCCTTATCTGATT
  TATCATCAGATTACCAGGAAGAGCAAATGAACAAATGCAAGTTAAACCAGATGTCCAAGTTGTACGACGA
  TGATGAACGTACTGATATTCAAAAGTCATACTGGAATGTGGTTAAGAAATTTGTGCGCATCCTTGTGTCC
  TCGGTTGCCTTCAGAATATTTGGAATTTTCCTGGTCATTCTGGATGTATTTCTTGTGGTTGTCGATCTAA
  ATGTCTCTGAAAAGAAAATTTATATTCCTTTGGACTATCGTTCCATTTCTTTAGCGATTGCTTTATTTTT
  CCTCGTGGATATTCTTCTTAGAGTGTCTGTAGAAGGGAGACGGCGCTATTTCTCTGACGTACTGAACACC
  CTAGACGCTGTGGTCATCGGTGTGACTGTGGTTGTGGCTGTCATTTATGCACTTTATGACAAACACTTTC
  TCAGAGATATCCCAAGACTGGCAGTTTTACTGCGGCCTCTACGACTGCTCATTCTGATAAGGATTCTGCA
  ACTGGCTCATCAAAAGAGACAACTCGAAAGGCTGACCAGGAAGCTGGTATCGGGTAACAAGCGACGCTAC
  AAGAAGGATGGCTTTGACCTAGACCTCACTTACGTCACTGAGCGTATTATTGCCATGTCATTTCCATCGT
  CTGGAAGGGAGTCCTTCTACAGAAATCCAATCAAGGAGGTTGTGCGGTTCCTGGATACCAAGCACCCAAA
  CCACTATCAAGTCTACAATCTATGCAGTGAGAGAGCTTATGACCCTAAGCACTTCCACTACAGGGTCCGC
  AGAATCATGATTGATGATCATAATGTCCCCACTCTTGAGGAAATGTTGTTGTTCTCCAAGGAAGTAAATA
  ATTGGATGGCTCAAGATCCTGAAAATGTTGTCGCGATTCACTGTAAGGGAGGAAAAGGAAGAACTGGAAC
  CATGGTGTGTGCCTGCCTCATTGCCAGTGAAATAGTTTTAAATGCAAAGGAAAGTCTGTACTTTTTTGGA
  GAGCGGCGGACAGATAAAAGCAACAGCTCGAAGTTCCAAGGAATAGAGACCCCTTCTCAGAATAGATATG
  TGAAATATTTTGAAAAACTGAAAATTAACTACCAACTAACTCTCCCTCCCAAAAAAGTACTCGTGATAAA
  AAGATTGGTCGTTTATTCCATTCATGGCGTTGGAAAAGGCGACGGGAGTGACCTGGAAGTACAAATCATA
  ATGTGGCAGGAGACTGTTTTTTCCTTTTGTAATTCCAGGAATTGTATGATATTTCATGACCCTGAAACAG
  ACAGAGCAATAATCAATGTGTTCCATTGCCCGGCTCTATACGATGATGTGAAAGTGAAATTTCTCTCTCC
  GAATCTTCCTAAATACTATGATGACTGCCCCTTTTTCTTTTGGTTTCATACATCTTTTATTAAAAATAAC
  AGGCTTTATCTACCAAGAAACGAATTGGATAATACCCATAAACCAAAAACATGGAAAATTTATGGTGAAA
  AGTTTGCAGTAGAGGTGGATTTTGGTGAGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214933 representing NM_199257
  Red=Cloning site Green=Tags(s)
  
  MYGEKKSHLYLWMEHYGYDMPANIYKMYSQPSRKTDDANKKVSVSASRTIKLNGSTGYDTNEQITLITNG
  SSLSYPDEIKSASYADPISTKAYTNDSSVYDPGGASSSTTLYELNSLSEVSKEIITQGESALLRDKEATS
  ELKIPSTLQTQTSMSTNTLSLSDLSSDYQEEQMNKCKLNQMSKLYDDDERTDIQKSYWNVVKKFVRILVS
  SVAFRIFGIFLVILDVFLVVVDLNVSEKKIYIPLDYRSISLAIALFFLVDILLRVSVEGRRRYFSDVLNT
  LDAVVIGVTVVVAVIYALYDKHFLRDIPRLAVLLRPLRLLILIRILQLAHQKRQLERLTRKLVSGNKRRY
  KKDGFDLDLTYVTERIIAMSFPSSGRESFYRNPIKEVVRFLDTKHPNHYQVYNLCSERAYDPKHFHYRVR
  RIMIDDHNVPTLEEMLLFSKEVNNWMAQDPENVVAIHCKGGKGRTGTMVCACLIASEIVLNAKESLYFFG
  ERRTDKSNSSKFQGIETPSQNRYVKYFEKLKINYQLTLPPKKVLVIKRLVVYSIHGVGKGDGSDLEVQII
  MWQETVFSFCNSRNCMIFHDPETDRAIINVFHCPALYDDVKVKFLSPNLPKYYDDCPFFFWFHTSFIKNN
  RLYLPRNELDNTHKPKTWKIYGEKFAVEVDFGEN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199257
• ORF Size: 1992 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199257.2, NP_954866.2
• RefSeq Size: 2549 bp
• RefSeq ORF: 1995 bp
• Locus ID: 234129
• Cytogenetics: 8 A2