Cspp1 (NM_026493) Mouse Tagged ORF Clone

CAT#: MG213626

Cspp1 (tGFP-tagged) - Mouse centrosome and spindle pole associated protein 1 (Cspp1), (10ug)

Product Images

Circular map for MG213626

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cspp1
• Synonyms: 2310020J12Rik; 4930413O22Rik; AA387550; BC055915; Cspp2; Cspp3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG213626 representing NM_026493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATAGCTTGGATGAATTTATTGAAGAGCAAAAAGCGAAACTGGCCAAAGACAAAGCAGAATTGG
  AAAGTGATCCACCGTACATGGAGATGAAGGGAAAGGCGTCAGAGAAACTCTCTGAAAACAGTAAAATATT
  AATCTCTATGGCTAAGGAGAACATTCCACCAAGCAGCCAACAACAGCCCAAGGGACCCTTAGGAATTGAA
  TATGGCTTAAGTTTACCGCTTGGAGAAGATTATGAACAGAAGAAACATAAATTAAAAGAAGAATTGAGGC
  AAGATTATAGACGATATCTTACTCAGAAAAATTTCCTATCTACTGGTGAAACAGACCCATCTACTCTGGG
  AGTTTCTCTTCCCATTGATGAGCGGTTATCTGCCAAGGAAAGATTGAAACTTGAACGCAACCGAGAATAC
  AATCAGTTTCTCAGGGGTAAGGCAGAATCCACAGAAAAAGTCAGACAGGTAGAAAAGAATATTGAGCCTA
  AGAGTCAAAGAAATAAAAACCCTATTAGTCAAGGTAAATCTGATCTACCTTTACAAATACAGACTGCATA
  CACACATTCAGAGGGACCCTGGCTAAGCCGGCAAGAGGAGGGCCTGTACCGACAGCTAGATGGTGAAATT
  GAATTAAGGAGCAGAAGACCGCTTAAACAAACAAAGGAAGAAGTAGGCATTTCTGGTGCAGAACATCCAA
  GCCTTTCTGGCAGCGCTGGTGTCCCAGAGAGAAGAGCCCGCAGGGCTAACGGGGAGCGGGTGCTTGACAG
  ACAACATTGCAGAGCCGACCGAGACCCTGGTGTGAGTGAAGACATGGACGAGAGGTTTAGATTTGAAAGT
  GATTTTGATAGAAGACTTTTGAGAGTGTATACAAATGGCAGGCCGCATGGGAGCAGACGAGGGTATGTGG
  ATGGGGATGATGTCCCAGAAGAGCCAAACACACAGATCTCAGCTGCTGAAAATAAAAGTGTTCACTGTAA
  TGGACCACCCAGATCTGCTGATCTCGATATCACAAGTCCATTTGCAGGCATGCTCTTTGGAGGTGAAGAT
  CGAGAACTTACCAAGAGAAGGAAGGAGAAATATAGGCAAGAACTACTAGAACAGATAGCTGAGCAACAGA
  AGAAGAAGAGACGAGAAAAAGATTTAGCATTTGGAATTACAACTTCTGGAGTACAAGACCCTGAAAAATC
  GCCTGATAGACTAAAGCAGTTTAGTTTGACACCAAGACACTTTGAAGAGATGCCACCTGAGAGGCCCAGA
  GTAGCTTTCCAGACACCTCCCCCTCCCTTCTCTGCCCCGTCTAGCCCATCCGTCCCACCAGTTCATTCCG
  CCCCTTCTCACAATGAAGACTTGCACAGTGGACTCGGCAGCACCCTTGGTGAACTGGCGCATCCCAGGGT
  TCTGCCTGTGCCTCTGAATCCACCCCCGCCTCCGCTCCTTGCCCCTCCAGCTTCTAACTATCGAACTCCT
  TACGATGATGCGTACTATTTTTACGGTGCCAGAAATACACTGGATCCCAATATTGTATACTATGGTTCAG
  GAATGATTGGAGGACAGCCTGCCCCTCATGTCTCTGCCCCTGTCACTCACCAGGTAGCACCGCCAGCTGT
  GAATACTGTTGGACAGAATGAACAGAAAGTTCTTAGTGATGGACTCAGGAATTCAGGACTGGTCTTTGAG
  GATAAGCCAAAACCTTCTACACAGTCGCTTCAGTCTTACCAAGAAGCTTTGCAGGAGCAGATTCGGGAAA
  GAGAAGCAAGAAGGAAGAAAGAACGTTTAGAAAAAGAAGAATATGAAGCTAAATTAGAAGCCGAAATGAG
  GATCTACAACCCCTGGGGGAAGGGCGGAGGTGGTGCTCCTCTCAGAGACGCCAAAGGAAATCTGATAACT
  GATTTGAATAGGATGCACAGACAGAATATAGATGCCTACCATAACCCAGATGCAAGAACATATGAAGATA
  AAAGGGCTGTTGTATCTATAGACCAAAATTTAGCCACTTCAAATGCTGAGAACCTAGAAGATTCTGCAAA
  TAAAAACTCAGGTCCTTTGCAAACACAAAGCTCTCCTTTTGCCCGGGGAAACACATTTGGTGAGCCACTC
  AGCGAGCTTCAGATTAAACAGCAAGAATTATACAAGAATTTTCTTCGTTTTCAGATTGAGGAAAAGAGAC
  AAAGAGAAGAAGCCGAGCGCGAGAAGCTGAGAGTTGCTGAAGAGAAGGAAGAAAAGCGGCTTGCGGAGCA
  GAGGGCAAGGATTCAGCAAGAGTATGAGGAGGAGCAGGAGCGGAGGAGGGAGAAGGAGGAGGAGCAAAGG
  TTGAAAAATGAAGAACTTATTCGGTTAGCTGAAGAGAGGCGAAAGGAAGCGGAAAGAAAGAAGAAAGAGG
  AGGAAGAAAAACATAACTTGCAACTTCAGCATTACTATGAGAGAGAAAATATTATCGGAGATGAAACAAA
  GCACTTGAGACAGCCTTCTCCTGTAGTTCCTGCTCTTCAGAACAAAATTGCAAGCAAACTCCAAAGACCT
  CCTTCAGTCGACACCATCATAAGTTCCTTCATTCACGAAAGCTCCATGTCCCGGGCACAGTCTCCTCCAG
  TGCCTGCAAGGAAGAACCAACTCCGTGCAGAAGAGGAGAAAAAAAATGTGATTATGGAGTTATCAGAAAT
  GAGGAAGCAGCTCCGGAGCGAGGAGAGGCGTTTGCAGGGGCGGCTGCTGCACCTGGACAGTGACGACGAG
  ATCCCCATGAGGAAAAGAGAGAGGAACCCAATGGATATATTTGACATGGCTAGACATCGGGTACAAGCCC
  CTGTCAGAAGACCATCGCCTAAGGGCTTAGATGCTACTACTTTTCAGAATATTCATGACTTTAATGAGCT
  GAGAGAGAGAGATTCAGATACTCGAGTGGATCTGAGATTAATGTACCCAGATCCTCCCCGCGATCATCAC
  ACCTTGGAGATTCAGCAGCAGGCCCTACTCCGAGAGCAGCAGAAGAGGCTTAATAGAATAAAGATGCGGA
  GAGATGCAGGAGCTGATTTGGACACCATCTGCACTGATAATGCACAAGGGCGCAGAATGCCCAGAGATGA
  CACTAATGATTTTTTGAAAAATTCATTACTAGAATCTGATAGTGCTTTTATTGGTGCTTATGGTGAGACA
  TATCCTGTTATTGAAGACAATGCCTTCCCTCCACCATCACAGCTGCCCTCTGCAAGGGAACGCAGGAGGA
  ACAAATTAAAAGGACTAGATTTTGACAGTAGTCGGCTGCACACCCCACAAGATGGTCTCTCTTTAAAATC
  TATCTCCAGTGTAAATGTCGATCAAGTTAGAATGAGAAATGAAGACAGAATGAGAAGACTGACTGAGCAG
  CAGAAGAAACCCACTAACACAGATGATGAGGGTTCACTGGTTGATCCTGATGACATCATGAGACACCTGA
  GTGATGATGGACGCAACTCTGCGGCAACTGAGCCCTGGCTCCGCCCTGGCACTTCAGAAAGTCTGAAACG
  GTTCATGGCAGAGCATCTGAATGAAGAACAGCATAAGGGGCCTGGGAAGCCAGGCACGTTTACTTGGCAG
  GGCCTCTCTGCTGCACATGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG213626 representing NM_026493
  Red=Cloning site Green=Tags(s)
  
  MADSLDEFIEEQKAKLAKDKAELESDPPYMEMKGKASEKLSENSKILISMAKENIPPSSQQQPKGPLGIE
  YGLSLPLGEDYEQKKHKLKEELRQDYRRYLTQKNFLSTGETDPSTLGVSLPIDERLSAKERLKLERNREY
  NQFLRGKAESTEKVRQVEKNIEPKSQRNKNPISQGKSDLPLQIQTAYTHSEGPWLSRQEEGLYRQLDGEI
  ELRSRRPLKQTKEEVGISGAEHPSLSGSAGVPERRARRANGERVLDRQHCRADRDPGVSEDMDERFRFES
  DFDRRLLRVYTNGRPHGSRRGYVDGDDVPEEPNTQISAAENKSVHCNGPPRSADLDITSPFAGMLFGGED
  RELTKRRKEKYRQELLEQIAEQQKKKRREKDLAFGITTSGVQDPEKSPDRLKQFSLTPRHFEEMPPERPR
  VAFQTPPPPFSAPSSPSVPPVHSAPSHNEDLHSGLGSTLGELAHPRVLPVPLNPPPPPLLAPPASNYRTP
  YDDAYYFYGARNTLDPNIVYYGSGMIGGQPAPHVSAPVTHQVAPPAVNTVGQNEQKVLSDGLRNSGLVFE
  DKPKPSTQSLQSYQEALQEQIREREARRKKERLEKEEYEAKLEAEMRIYNPWGKGGGGAPLRDAKGNLIT
  DLNRMHRQNIDAYHNPDARTYEDKRAVVSIDQNLATSNAENLEDSANKNSGPLQTQSSPFARGNTFGEPL
  SELQIKQQELYKNFLRFQIEEKRQREEAEREKLRVAEEKEEKRLAEQRARIQQEYEEEQERRREKEEEQR
  LKNEELIRLAEERRKEAERKKKEEEEKHNLQLQHYYERENIIGDETKHLRQPSPVVPALQNKIASKLQRP
  PSVDTIISSFIHESSMSRAQSPPVPARKNQLRAEEEKKNVIMELSEMRKQLRSEERRLQGRLLHLDSDDE
  IPMRKRERNPMDIFDMARHRVQAPVRRPSPKGLDATTFQNIHDFNELRERDSDTRVDLRLMYPDPPRDHH
  TLEIQQQALLREQQKRLNRIKMRRDAGADLDTICTDNAQGRRMPRDDTNDFLKNSLLESDSAFIGAYGET
  YPVIEDNAFPPPSQLPSARERRRNKLKGLDFDSSRLHTPQDGLSLKSISSVNVDQVRMRNEDRMRRLTEQ
  QKKPTNTDDEGSLVDPDDIMRHLSDDGRNSAATEPWLRPGTSESLKRFMAEHLNEEQHKGPGKPGTFTWQ
  GLSAAHA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026493
• ORF Size: 3591 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026493.3, NP_080769.3
• RefSeq Size: 4378 bp
• RefSeq ORF: 3594 bp
• Locus ID: 211660
• UniProt ID: B2RX88
• Cytogenetics: 1 A2
• Gene Summary: May play a role in cell-cycle-dependent microtubule organization.[UniProtKB/Swiss-Prot Function]