Rnf123 (BC057082) Mouse Tagged ORF Clone

CAT#: MG211921

  • TrueORF®

Rnf123 (tGFP-tagged) - Mouse ring finger protein 123 (cDNA clone MGC:67486 IMAGE:5704926)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "BC057082" in other vectors (4)

Reconstitution Protocol

USD 1,349.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

USD 750.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

USD 412.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "Rnf123"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Rnf123
Synonyms BC003945; Kpc1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211921 representing BC057082
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCAAGGGGACTGGCATGTCGTTCTCCCGAAAGAGCTATAGGCTGACCTCAGATGCTGAGAAGT
CCAGGGTCACAGGCATCGTGCAAGAGAAACTACTGAGCGACTATCTGTACCGCATCTTTTCCCCTCCTGA
CCGTGGACCCGCCGCAGCCACCAGCAGGAAACCGCTAAACTTCCATAACCTGCCTGAGCACGTGGACCAG
CTGCTACAGGTGGACAGTGAAGACAACGAGAGCCAGGGACAAGTTGAAGGTCGACTTGGCCCATCTACTG
TGGTCCTAGACCACACAGGAGGCTTTGAGGGGCTTCTCCTTGTGGATGATGACCTCCTGGGGGTGATTGG
ACACAGCAACTTTGGCACTATCCGTTCTACCACATGTGTGTACAAAGGGAAGTGGGTCTACGAGGTGCTC
ATCTCCTCCCAGGGCCTCATGCAGATCGGCTGGTGCACCATCAACTGCCGCTTTAATCAGGAGGAAGGGG
TTGGAGACACACATAACTCCTATGCCTATGACGGCAACCGAGTGCGCAAGTGGAATGTTACCACCACGAA
TTATGGCAAGGCGTGGGCTGCGGGGGACATTGTCAGCTGCCTAATTGATCTGGATGATGGGACTCTGTCC
TTCTGCCTGAATGGCGTGTCACTGGGCACTGCCTTCGAGAACCTTTCCAGGGGCCTAGGAATGGCGTACT
TCCCAGCCATCAGCCTGTCATTCAAGGAGTCTGTGGCATTCAACTTTGGCAGCCGTCCTTTGCGCTACCC
AGTTGCGGGCTTCCGGCCCCTGCAGGACCCTCCGTTTGCTGACCTGGTCCGGGCACAGAGGTTGCTGGGC
TGCTTCCAGGCAGTGCTAAGTGTGGAGCTGGACCCTGTGGAAGGGCGGCTGGTGGAGACGGAGAGCTCTG
AGTGGCAGCTGCAAGGGCAGCCCACTGTCCTCCTCACGCTGGCCCACATCTTCCATCACTTTGCACCACT
GCTGCGCAAGGTATACCTGGTGGAGGCTGTGCTAATGAGCTTCCTGCTGGGCGTTGTGGAGAAGGGCACA
CCAGAGCAGGCGCAGTCTGTGGTACACCAGATCTTGGACCTCTTGTGGCTCTTCATGGAGGACTATGAGG
TACAGGATTGCCTGAAGCAGTTGATGATGTCACTTCTACGTCTCTACCGATTCTCGCCTATTGTCCCAGA
CCTGGGTCTACAGATCCACTACCTGCGCCTCACTATGTCCATCCTGAGACACGAGAAGTCCCGCAAGTTC
CTGCTTAGCAATGTCCTTTTTGACATGCTCCGGTCCGTGGTCTTCTTTTATATTAAGAGTCCCCTGCGTG
TGGAGGAAGCTGGCCTGAAGGAACTCATTCCCACCACCTGGTGGCCCCATCGCTCCAGCAGGGAGAGCAG
AGACGGTAAGGAAGCAAGGGAGGAGACCACCGAAGAGCGGCAGCGGAGGCGAGCCTATGAGCGTGGCTGC
CAAAGACTCAAGAAACGCATTGAAGTGGTGGAAGAACTGCAGGTCCAGATCCTGAAGCTGCTGTTGGACA
ATAAAGATGACAATGGGGGTGAAGCTTCTAGGTACATCTTTCTGACAAAATTCCGAAAGTTCCTGCAGGA
GAATGCCAGCGGCCGGGGGAACACACCCGTGCTCTGCCCCCCTGAGTACATGGTCTGCTTCCTACACCGG
CTGGTGTCTGCCTTGCGCTTCTATTGGGATGAATACAAAGCTTCCAACCCCCGTGCTTCCTTCAGTGAGG
AGGCTTACATCCCGCCCCAGATCTTCTATAATGGCAAGGTGGACTACTTTGACCTTCAGCGCCTTGGGGG
CCTCCTCTCACACCTTCGAAAGACCCTTAAAGATGACCTTGCTTCCAAAGCCAACATCGTGATCGACCCC
CTGGAGCTCCAGGCAGCCACCATGGATGACCTGGATGAGGATGAAGAGCCTGCCCCCTCAGCGGCCCAGG
TGTGGCAGGAGGGACAGCGTCCGATGCAAGCCCTGGCCATCGGAGGGGCACTGCCCCTGCCCCGGCCAGG
CTGGCTCAGTTCTCCAACCCTGGGCAGAGCCAACCGCTTCCTCAGCACGGCAGCTGTGAGCCTCATGACC
CCACGGCGGCTTCTGAGCACCATGGAGAAAGTCAAAGTTCGCTCACTGAATGTGGAACAGAGGACCCGTG
AGGACATTGAGGGCAGCCACTGGAATGAGGGCCTGCTGTTGGGGAGGCCCCCTGAAGAGCCTGAGCAGCC
GCTTACCGAGAACTCGCTGTTGGAAGTCCTGGATGGCACAGTCATGATGTATAACCTCAGCGTTCACCAG
CAGCTGGGCAAGATGGTGGGTGTGTCTGATGATGTCAACGAGTATGCAATGGCCCTAAGAGACACAGAGG
ACAAGCTCCGTCGGTGCCCTAAGAGGAGGAAGGATATCCTTGCAGAGTTGACCAAGAGCCAGAAGGTTTT
CTCAGAAAAGCTGGACCACCTGAGCCGCAGGCTTGCCTGGGTCCACGCCACAGTCTACTCACAGGAGAAA
ATGCTGGATATCTACTGGTTACTGCGTGTCTGCCTACGGACCATTGAGCATGGGGACCGCACGGGGTCTC
TCTTTGCCTTCATGCCTGAGTTCTACCTAAGTGTGGCTATCAACAGCTACAGTGCCCTGAAGAACTATTT
TGGCCCTGTGCACAGCATGGAGGAACTCCCAGGCTATGAAGAGACCCTGACACGCTTAGCTGCCATCCTC
GCCAAACACTTTGCTGACCCTCGAATAGTAGGCACTGATATTCGAGACTCACTGATGCAGGCCCTGGCCA
GCTATGTGTGCTACCCACACTCCCTGCGGGCTGTGGAACGGATTCCTGAGGAACAGCGCATCGCCATGGT
GAGGAACCTTTTGGCACCCTATGAGCAACGGCCCTGGGCCCAGACCAACTGGATCCTGGTGCGGCTTTGG
AGGGGCTGTGGGTTTGGGTACCGCTATACACGGCTGCCACATCTGCTGAAAACCAAGCCAGAGGATGCCA
ATTTGCCCAGCCTCCAAAAGCCCTGCCCTTCGACCTTGCTACAGCAGCACATGGCGGACCTGCTGCGACA
AGGGTCTGATGTGGCACCGAGCTTCCTCAACAGTGTCCTTAACCAGCTCAACTGGGCCTTCTCTGAGTTC
ATCGGCATGATCCAGGAGATTCAACAGGCTGCTGAACGCCTGGAGCGGAACTTTGTGGACAGCCGACAGC
TCAAGGTCTGTGCCACCTGCTTTGACCTGTCGGTCAGCTTGTTGCGCGTCTTGGAAATGACCATCACGCT
GGTACCTGAAATATTCCTTGACTGGTCCCGCCCTACCTCTGAGATGCTGCTTCGGCGTCTGGCACAGCTG
CTGAACCAGGTGCTGAACCGGGTGACAGCTGAGAGGAACCTGTTTGACCGTGTAGTTACCCTACGGCTAC
CTGGGCTGGAGAGTGTGGACCACTACCCTATCCTGGTGGCAGTGACTGGCATCCTGGTACGCCTCCTGGT
GCACGGCCCAACCTCAGAGACAGAGCAAGCCACCTCTGTGCTCCTGGCTGATCCCTGCTTCCAGCTTCGT
TCCATATGCTATCTCCTGGGGCAGCCAGAGCCCCTAGCACCTGGCACTACCTTGCCTGCCCCTGACCGGA
AACGCTTCTCTCTACAGAGTTATACAGATTATATCAGCGCTGAGGAGCTGGCCCAGGTGGAACAGATGCT
GGCTCACCTGACCGCTGCATCTGCCCAGGCGGCCGCCGCCTCCCTGCCCACCAATGAAGAGGACCTCTGC
CCAATCTGCTACGCCCACCCCATCTCTGCTGTGTTCCAGCCTTGTGGTCACAAATCCTGCAAAGCCTGCA
TCAACCAGCACCTGATGAACAACAAGGACTGCTTCTTCTGCAAAGCCACCATTGTATCTGTAGAGGACTG
GGACAAGGCAGCCAACACAAGCGCCATGTCCTCAGCTGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211921 representing BC057082
Red=Cloning site Green=Tags(s)

MASKGTGMSFSRKSYRLTSDAEKSRVTGIVQEKLLSDYLYRIFSPPDRGPAAATSRKPLNFHNLPEHVDQ
LLQVDSEDNESQGQVEGRLGPSTVVLDHTGGFEGLLLVDDDLLGVIGHSNFGTIRSTTCVYKGKWVYEVL
ISSQGLMQIGWCTINCRFNQEEGVGDTHNSYAYDGNRVRKWNVTTTNYGKAWAAGDIVSCLIDLDDGTLS
FCLNGVSLGTAFENLSRGLGMAYFPAISLSFKESVAFNFGSRPLRYPVAGFRPLQDPPFADLVRAQRLLG
CFQAVLSVELDPVEGRLVETESSEWQLQGQPTVLLTLAHIFHHFAPLLRKVYLVEAVLMSFLLGVVEKGT
PEQAQSVVHQILDLLWLFMEDYEVQDCLKQLMMSLLRLYRFSPIVPDLGLQIHYLRLTMSILRHEKSRKF
LLSNVLFDMLRSVVFFYIKSPLRVEEAGLKELIPTTWWPHRSSRESRDGKEAREETTEERQRRRAYERGC
QRLKKRIEVVEELQVQILKLLLDNKDDNGGEASRYIFLTKFRKFLQENASGRGNTPVLCPPEYMVCFLHR
LVSALRFYWDEYKASNPRASFSEEAYIPPQIFYNGKVDYFDLQRLGGLLSHLRKTLKDDLASKANIVIDP
LELQAATMDDLDEDEEPAPSAAQVWQEGQRPMQALAIGGALPLPRPGWLSSPTLGRANRFLSTAAVSLMT
PRRLLSTMEKVKVRSLNVEQRTREDIEGSHWNEGLLLGRPPEEPEQPLTENSLLEVLDGTVMMYNLSVHQ
QLGKMVGVSDDVNEYAMALRDTEDKLRRCPKRRKDILAELTKSQKVFSEKLDHLSRRLAWVHATVYSQEK
MLDIYWLLRVCLRTIEHGDRTGSLFAFMPEFYLSVAINSYSALKNYFGPVHSMEELPGYEETLTRLAAIL
AKHFADPRIVGTDIRDSLMQALASYVCYPHSLRAVERIPEEQRIAMVRNLLAPYEQRPWAQTNWILVRLW
RGCGFGYRYTRLPHLLKTKPEDANLPSLQKPCPSTLLQQHMADLLRQGSDVAPSFLNSVLNQLNWAFSEF
IGMIQEIQQAAERLERNFVDSRQLKVCATCFDLSVSLLRVLEMTITLVPEIFLDWSRPTSEMLLRRLAQL
LNQVLNRVTAERNLFDRVVTLRLPGLESVDHYPILVAVTGILVRLLVHGPTSETEQATSVLLADPCFQLR
SICYLLGQPEPLAPGTTLPAPDRKRFSLQSYTDYISAEELAQVEQMLAHLTAASAQAAAASLPTNEEDLC
PICYAHPISAVFQPCGHKSCKACINQHLMNNKDCFFCKATIVSVEDWDKAANTSAMSSAA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057082
ORF Size 3960 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057082.1
RefSeq Size 4745 bp
RefSeq ORF 3962 bp
Locus ID 84585
Cytogenetics 9 F1
Gene Summary Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.