Iars (NM_172015) Mouse Tagged ORF Clone
CAT#: MG211866
- TrueORF®
Iars (tGFP-tagged) - Mouse isoleucine-tRNA synthetase (Iars)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_172015" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Iars |
Synonyms | 2510016L12Rik; AI327140; AU044614; E430001P04Rik; ILRS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211866 representing NM_172015
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCAACAAGTTCCAGAAAACATCAGTTTTCCTGCTGAAGAAGAAAAAATCTTGGAGTTCTGGTCCA AACATAACTGTTTCCAGGAATGCCTCAAACAGTCAAAACTCAGGCCAAAGTTTACCTTCTATGATGGGCC TCCCTTTGCTACTGGACTGCCTCACTATGGACACATACTTGCTGGGACAATCAAAGATATCGTTACGAGA TACGCTCACCAGAGCGGGTTTCACGTTGACAGAAGATTCGGGTGGGACTGTCACGGCTTACCTGTGGAGT ACGAAATCGATAAGACACTGGGCATCAAAGGCCCGGAGGATGTGGCCAAAATGGGGATTGCAGAGTACAA CAAGCAGTGCCGAGCAATCGTGATGAGATACTCCGCTGAGTGGAAGTCTACTGTTACCAGACTCGGCCGA TGGATCGACTTTGACAATGACTACAAAACACTATACCCACAGTTCATGGAATCAGTCTGGTGGGTCTTCA AGCAGCTGTATGACAAAGGCCTCGTGTACAGAGGCGTGAAGGTCATGCCCTTCTCCACCGCGTGTGGCAC TCCACTCTCCAACTTCGAATCTAACCAGAATTACAAGGATGTTCAGGATCCCTCAGTATTTGTAACATTC CCTTTGGAGGAAGATGAAAATACATCCTTAGTTGCTTGGACAACCACTCCTTGGACTCTACCCAGTAACC TTGCACTATGCGTCAATCCAGAAATACAGTATGTGAAGATCAAAGATGTGGCCAGGGGAAAATTATTCAT CTTAACGGAAGCCAGATTATCGGCCCTCTATAAGCAAGAGAGCGACTATGAGATACTTGAAAGGTTTCCT GGTGCCTCTCTCAAAGGCAAGAAATACAAGCCGTTGTTTGACTACTTTATTAAGTGTAAGGAGAACGGTG CCTTCACCGTGCTTGTCGACCACTATGTGAAGGATGAAGAAGGCACAGGCGTTGTGCACCAGGCTCCTTA CTTTGGTGCTGATGATCATCGTGTCTGTATGGACTTCAACATTATTCAGAAAGACTCAGTCCCTGTTTGT CCTGTGGATGCTTCAGGCTGCTTCACAGAGGAAGTGACACATTTTGTGGGGCAGTATGTGAAGGATGCTG ACAAAAATATCATTAGGATGTTGAAGGAGCAGGGCCGGCTGCTGGCGGCGGGCACGTTCACTCACAGCTA CCCTTTCTGCTGGAGGTCAGACACGCCCCTCATTTACAAGTCTGTGCCCAGCTGGTTTGTGCGGGTGGAG CCCATGGTGGACCAGCTACTGAAGAACAACGACCTGTGCTACTGGGTCCCAGAGTTTGTACGAGAAAAAC GGTTTGGAAATTGGCTGAAGGAGGCCCGTGACTGGGCAATTTCCAGAAACAGATACTGGGGCACCCCCAT CCCACTGTGGGTCAGCGAAGACTTGGAGGAGGTAGTGTGCATTGGATCAGTGGCAGAGCTTGAAGAACTG TCGGGAACAAAAATCTCAGATCTCCACAGAGAGAGCATCGACCACCTGACCATCCCATCCCGCTGCGGGA AAGCACCATTGCGGCGCGTCTCTGAGGTGTTTGACTGCTGGTTCGAGAGTGGAAGTATGCCCTATGCCCA GGTCCATTATCCGTTCGAGAGCAAGCGCGAGTTTGAGGATGCCTTTCCTGCAGACTTCATTGCTGAAGGC ATCGATCAAACCAGAGGATGGTTTTACACCCTGCTGGTGCTAGCCACAGCGCTCTTTGGACAGCCACCTT TCAAGAATGTGATTGTGAATGGCCTCATCTTGGCAAGTGATGGCCAAAAAATGAGCAAGCGGAAAAAGAA CTACCCAGATCCAGTTTCCATCATCGATAAGTATGGTGCGGACGCCCTCAGGTTATATCTGATCAATTCC CCTGTGGTAAGAGCAGAAAACCTGCGCTTCAAGGAGGAAGGTGTGCGGGATGTCCTCAAAGATGTGCTGC TCCCGTGGTACAACGCTTACCGCTTCTTCATCCAGAACGTCTTTAGGCTCCACAAGGAAGAAGAAGTGAA GTTCCTATACAACGAGCATACAGTCAGAGAAAGCCCTAACATCACCGACCGCTGGGTTCTGTCCTTCATG CAGTCGCTCCTTGGCTTCTTTGAGACCGAAATGGCAGCTTACAGGCTGTACACTGTGGTACCTCGCCTGG TCAAGTTTGTAGATATTCTAACCAACTGGTATGTCCGAATGAACAGGAGGAGGTTAAAGGGTGAGAGTGG GGTGGAAGACTGTGTCATGGCTCTGGAGACACTGTTCAGTGTCCTGCTTTCTCTGTGCAGACTGATGGCC CCTTACACGCCCTTTCTGACTGAGCTGATGTACCAGAATCTAAAGCTGCTGATTGATCCTGCTTCTCTCC GGGACAAGGACACACTCAGCATCCACTACCTCATGCTGCCTCGAGTTCGAGAGGAGCTGATTGACAAGAA AACTGAGAACGCCGTGTCTAGGATGCAGTCTGTGATCGAGCTTGGAAGAGTGATACGAGACCGAAAAACT ATTCCAATAAAGTATCCTCTGAAGGAAATTGTGGTCATCCACCAAGACCCAGAAGCTCTAGAGGATATCA GGTCCTTGGAGAAGTATATCATTGAGGAATTAAATGTTCGAAAAGTCACGCTGTCTACTGATAAAAACAA GTACGGTATCCGACTAAGGGCTGAACCAGATCACATGGTCCTTGGGAAGCGCCTGAAGGGGGCCTTCAAA GCGGTGATGATGGCCATAAAGCGGCTGAGCAATGAGGAGCTGGAGCGGTTTCAGAAGAGCGGGAGCATTG TTGTGGAAGGCCACGAACTGCATGAGGAAGACATTCGCCTCATGTACACCTTTGACCAGGCCACGGGAGG GACCGCGCAGTTTGAAGCACACTCTGATGCTCAGGCTCTAGTTCTCTTAGATGTCACACCTGACCAGTCA ATGGTGGATGAGGGCATGGCTCGGGAAGTCATCAATCGCATCCAGAAGCTTCGAAAGAAGTGCAATCTGG TTCCAACGGATGAAATAACAGTTTACTATAATGCAAAATCTGAAGGAAGATACTTGAATAATGTCATTGA AAGCCACACTGACTTCATATTTGCCACAATCAAAGCTCCCTTGAAGCCATATCCAGTTCCAACATCAGAT AATATCCTTATTCAAGAGCAAACACAGTTAAAGGGGTCCGAGCTGGAAATCACGCTCACCAAAGGATCGT GTGTGCCTGGCCCTGCTTGTGCTTATGTGAACCTTAACATTTGTGCAAATGGCACCGAGCAAGGTGGAGT GTTGCTCCTGGAGAATCCGAAAGGTGATAATCAGCTGAACCTGGTGAAACTGAAGACTGTTGTTACCAGC GTATTTGGTGTTAAAAATGCAAAGCTGTCAGTCTTCCACGGTGAAACAGAAATACAGAACCAAACTGACT TGCTGAGCCTCAGTGGGAGGACGCTGTGTGTGACTGCAGGGGCATCCCCGTCTCCCATCAGCAGCCCCAG CACCCTGCTGTGTCAGTACCTCAACCTGCAGCTCCTGAACGCAGAGCCACAAGAGTGTTTAACAGGAACT GTGGGCACCCTCCTGCTTGAAAACCCACTCGGACAGAACGGCCTCACCCACCAAGGGCTTGTGCATGAAG CAGCCAAGGTGTTCGGACTGAGGAGCAGGAGGCTAAGGCTGTTCCTGAATGAGACTCAAACACAAGAAAT TACAGAAGATATCCCAATGAAGACTTTGAATATGAAGACTGTATATGTTTCTGTCTTGCCAACAACTGCA GATGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211866 representing NM_172015
Red=Cloning site Green=Tags(s) MVQQVPENISFPAEEEKILEFWSKHNCFQECLKQSKLRPKFTFYDGPPFATGLPHYGHILAGTIKDIVTR YAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNKQCRAIVMRYSAEWKSTVTRLGR WIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACGTPLSNFESNQNYKDVQDPSVFVTF PLEEDENTSLVAWTTTPWTLPSNLALCVNPEIQYVKIKDVARGKLFILTEARLSALYKQESDYEILERFP GASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDHRVCMDFNIIQKDSVPVC PVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLAAGTFTHSYPFCWRSDTPLIYKSVPSWFVRVE PMVDQLLKNNDLCYWVPEFVREKRFGNWLKEARDWAISRNRYWGTPIPLWVSEDLEEVVCIGSVAELEEL SGTKISDLHRESIDHLTIPSRCGKAPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEG IDQTRGWFYTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIIDKYGADALRLYLINS PVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVKFLYNEHTVRESPNITDRWVLSFM QSLLGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGESGVEDCVMALETLFSVLLSLCRLMA PYTPFLTELMYQNLKLLIDPASLRDKDTLSIHYLMLPRVREELIDKKTENAVSRMQSVIELGRVIRDRKT IPIKYPLKEIVVIHQDPEALEDIRSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFK AVMMAIKRLSNEELERFQKSGSIVVEGHELHEEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQS MVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYLNNVIESHTDFIFATIKAPLKPYPVPTSD NILIQEQTQLKGSELEITLTKGSCVPGPACAYVNLNICANGTEQGGVLLLENPKGDNQLNLVKLKTVVTS VFGVKNAKLSVFHGETEIQNQTDLLSLSGRTLCVTAGASPSPISSPSTLLCQYLNLQLLNAEPQECLTGT VGTLLLENPLGQNGLTHQGLVHEAAKVFGLRSRRLRLFLNETQTQEITEDIPMKTLNMKTVYVSVLPTTA DG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_172015 |
ORF Size | 3786 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172015.3, NP_742012.2 |
RefSeq Size | 4396 bp |
RefSeq ORF | 3789 bp |
Locus ID | 105148 |
UniProt ID | Q8BU30 |
Cytogenetics | 13 A5 |
Gene Summary | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205234 | Iars (untagged) - Mouse isoleucine-tRNA synthetase (Iars), (10ug) |
USD 1,099.00 |
|
MR211866 | Iars (Myc-DDK-tagged) - Mouse isoleucine-tRNA synthetase (Iars) |
USD 1,098.00 |
|
MR211866L3 | Lenti ORF clone of Iars (Myc-DDK-tagged) - Mouse isoleucine-tRNA synthetase (Iars) |
USD 1,398.00 |
|
MR211866L4 | Lenti ORF clone of Iars (mGFP-tagged) - Mouse isoleucine-tRNA synthetase (Iars) |
USD 1,398.00 |
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