Iars (NM_172015) Mouse Tagged ORF Clone

CAT#: MG211866

Iars (tGFP-tagged) - Mouse isoleucine-tRNA synthetase (Iars)

Product Images

Circular map for MG211866

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Iars
• Synonyms: 2510016L12Rik; AI327140; AU044614; E430001P04Rik; ILRS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211866 representing NM_172015
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCAACAAGTTCCAGAAAACATCAGTTTTCCTGCTGAAGAAGAAAAAATCTTGGAGTTCTGGTCCA
  AACATAACTGTTTCCAGGAATGCCTCAAACAGTCAAAACTCAGGCCAAAGTTTACCTTCTATGATGGGCC
  TCCCTTTGCTACTGGACTGCCTCACTATGGACACATACTTGCTGGGACAATCAAAGATATCGTTACGAGA
  TACGCTCACCAGAGCGGGTTTCACGTTGACAGAAGATTCGGGTGGGACTGTCACGGCTTACCTGTGGAGT
  ACGAAATCGATAAGACACTGGGCATCAAAGGCCCGGAGGATGTGGCCAAAATGGGGATTGCAGAGTACAA
  CAAGCAGTGCCGAGCAATCGTGATGAGATACTCCGCTGAGTGGAAGTCTACTGTTACCAGACTCGGCCGA
  TGGATCGACTTTGACAATGACTACAAAACACTATACCCACAGTTCATGGAATCAGTCTGGTGGGTCTTCA
  AGCAGCTGTATGACAAAGGCCTCGTGTACAGAGGCGTGAAGGTCATGCCCTTCTCCACCGCGTGTGGCAC
  TCCACTCTCCAACTTCGAATCTAACCAGAATTACAAGGATGTTCAGGATCCCTCAGTATTTGTAACATTC
  CCTTTGGAGGAAGATGAAAATACATCCTTAGTTGCTTGGACAACCACTCCTTGGACTCTACCCAGTAACC
  TTGCACTATGCGTCAATCCAGAAATACAGTATGTGAAGATCAAAGATGTGGCCAGGGGAAAATTATTCAT
  CTTAACGGAAGCCAGATTATCGGCCCTCTATAAGCAAGAGAGCGACTATGAGATACTTGAAAGGTTTCCT
  GGTGCCTCTCTCAAAGGCAAGAAATACAAGCCGTTGTTTGACTACTTTATTAAGTGTAAGGAGAACGGTG
  CCTTCACCGTGCTTGTCGACCACTATGTGAAGGATGAAGAAGGCACAGGCGTTGTGCACCAGGCTCCTTA
  CTTTGGTGCTGATGATCATCGTGTCTGTATGGACTTCAACATTATTCAGAAAGACTCAGTCCCTGTTTGT
  CCTGTGGATGCTTCAGGCTGCTTCACAGAGGAAGTGACACATTTTGTGGGGCAGTATGTGAAGGATGCTG
  ACAAAAATATCATTAGGATGTTGAAGGAGCAGGGCCGGCTGCTGGCGGCGGGCACGTTCACTCACAGCTA
  CCCTTTCTGCTGGAGGTCAGACACGCCCCTCATTTACAAGTCTGTGCCCAGCTGGTTTGTGCGGGTGGAG
  CCCATGGTGGACCAGCTACTGAAGAACAACGACCTGTGCTACTGGGTCCCAGAGTTTGTACGAGAAAAAC
  GGTTTGGAAATTGGCTGAAGGAGGCCCGTGACTGGGCAATTTCCAGAAACAGATACTGGGGCACCCCCAT
  CCCACTGTGGGTCAGCGAAGACTTGGAGGAGGTAGTGTGCATTGGATCAGTGGCAGAGCTTGAAGAACTG
  TCGGGAACAAAAATCTCAGATCTCCACAGAGAGAGCATCGACCACCTGACCATCCCATCCCGCTGCGGGA
  AAGCACCATTGCGGCGCGTCTCTGAGGTGTTTGACTGCTGGTTCGAGAGTGGAAGTATGCCCTATGCCCA
  GGTCCATTATCCGTTCGAGAGCAAGCGCGAGTTTGAGGATGCCTTTCCTGCAGACTTCATTGCTGAAGGC
  ATCGATCAAACCAGAGGATGGTTTTACACCCTGCTGGTGCTAGCCACAGCGCTCTTTGGACAGCCACCTT
  TCAAGAATGTGATTGTGAATGGCCTCATCTTGGCAAGTGATGGCCAAAAAATGAGCAAGCGGAAAAAGAA
  CTACCCAGATCCAGTTTCCATCATCGATAAGTATGGTGCGGACGCCCTCAGGTTATATCTGATCAATTCC
  CCTGTGGTAAGAGCAGAAAACCTGCGCTTCAAGGAGGAAGGTGTGCGGGATGTCCTCAAAGATGTGCTGC
  TCCCGTGGTACAACGCTTACCGCTTCTTCATCCAGAACGTCTTTAGGCTCCACAAGGAAGAAGAAGTGAA
  GTTCCTATACAACGAGCATACAGTCAGAGAAAGCCCTAACATCACCGACCGCTGGGTTCTGTCCTTCATG
  CAGTCGCTCCTTGGCTTCTTTGAGACCGAAATGGCAGCTTACAGGCTGTACACTGTGGTACCTCGCCTGG
  TCAAGTTTGTAGATATTCTAACCAACTGGTATGTCCGAATGAACAGGAGGAGGTTAAAGGGTGAGAGTGG
  GGTGGAAGACTGTGTCATGGCTCTGGAGACACTGTTCAGTGTCCTGCTTTCTCTGTGCAGACTGATGGCC
  CCTTACACGCCCTTTCTGACTGAGCTGATGTACCAGAATCTAAAGCTGCTGATTGATCCTGCTTCTCTCC
  GGGACAAGGACACACTCAGCATCCACTACCTCATGCTGCCTCGAGTTCGAGAGGAGCTGATTGACAAGAA
  AACTGAGAACGCCGTGTCTAGGATGCAGTCTGTGATCGAGCTTGGAAGAGTGATACGAGACCGAAAAACT
  ATTCCAATAAAGTATCCTCTGAAGGAAATTGTGGTCATCCACCAAGACCCAGAAGCTCTAGAGGATATCA
  GGTCCTTGGAGAAGTATATCATTGAGGAATTAAATGTTCGAAAAGTCACGCTGTCTACTGATAAAAACAA
  GTACGGTATCCGACTAAGGGCTGAACCAGATCACATGGTCCTTGGGAAGCGCCTGAAGGGGGCCTTCAAA
  GCGGTGATGATGGCCATAAAGCGGCTGAGCAATGAGGAGCTGGAGCGGTTTCAGAAGAGCGGGAGCATTG
  TTGTGGAAGGCCACGAACTGCATGAGGAAGACATTCGCCTCATGTACACCTTTGACCAGGCCACGGGAGG
  GACCGCGCAGTTTGAAGCACACTCTGATGCTCAGGCTCTAGTTCTCTTAGATGTCACACCTGACCAGTCA
  ATGGTGGATGAGGGCATGGCTCGGGAAGTCATCAATCGCATCCAGAAGCTTCGAAAGAAGTGCAATCTGG
  TTCCAACGGATGAAATAACAGTTTACTATAATGCAAAATCTGAAGGAAGATACTTGAATAATGTCATTGA
  AAGCCACACTGACTTCATATTTGCCACAATCAAAGCTCCCTTGAAGCCATATCCAGTTCCAACATCAGAT
  AATATCCTTATTCAAGAGCAAACACAGTTAAAGGGGTCCGAGCTGGAAATCACGCTCACCAAAGGATCGT
  GTGTGCCTGGCCCTGCTTGTGCTTATGTGAACCTTAACATTTGTGCAAATGGCACCGAGCAAGGTGGAGT
  GTTGCTCCTGGAGAATCCGAAAGGTGATAATCAGCTGAACCTGGTGAAACTGAAGACTGTTGTTACCAGC
  GTATTTGGTGTTAAAAATGCAAAGCTGTCAGTCTTCCACGGTGAAACAGAAATACAGAACCAAACTGACT
  TGCTGAGCCTCAGTGGGAGGACGCTGTGTGTGACTGCAGGGGCATCCCCGTCTCCCATCAGCAGCCCCAG
  CACCCTGCTGTGTCAGTACCTCAACCTGCAGCTCCTGAACGCAGAGCCACAAGAGTGTTTAACAGGAACT
  GTGGGCACCCTCCTGCTTGAAAACCCACTCGGACAGAACGGCCTCACCCACCAAGGGCTTGTGCATGAAG
  CAGCCAAGGTGTTCGGACTGAGGAGCAGGAGGCTAAGGCTGTTCCTGAATGAGACTCAAACACAAGAAAT
  TACAGAAGATATCCCAATGAAGACTTTGAATATGAAGACTGTATATGTTTCTGTCTTGCCAACAACTGCA
  GATGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211866 representing NM_172015
  Red=Cloning site Green=Tags(s)
  
  MVQQVPENISFPAEEEKILEFWSKHNCFQECLKQSKLRPKFTFYDGPPFATGLPHYGHILAGTIKDIVTR
  YAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNKQCRAIVMRYSAEWKSTVTRLGR
  WIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACGTPLSNFESNQNYKDVQDPSVFVTF
  PLEEDENTSLVAWTTTPWTLPSNLALCVNPEIQYVKIKDVARGKLFILTEARLSALYKQESDYEILERFP
  GASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDHRVCMDFNIIQKDSVPVC
  PVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLAAGTFTHSYPFCWRSDTPLIYKSVPSWFVRVE
  PMVDQLLKNNDLCYWVPEFVREKRFGNWLKEARDWAISRNRYWGTPIPLWVSEDLEEVVCIGSVAELEEL
  SGTKISDLHRESIDHLTIPSRCGKAPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEG
  IDQTRGWFYTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIIDKYGADALRLYLINS
  PVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVKFLYNEHTVRESPNITDRWVLSFM
  QSLLGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGESGVEDCVMALETLFSVLLSLCRLMA
  PYTPFLTELMYQNLKLLIDPASLRDKDTLSIHYLMLPRVREELIDKKTENAVSRMQSVIELGRVIRDRKT
  IPIKYPLKEIVVIHQDPEALEDIRSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFK
  AVMMAIKRLSNEELERFQKSGSIVVEGHELHEEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQS
  MVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYLNNVIESHTDFIFATIKAPLKPYPVPTSD
  NILIQEQTQLKGSELEITLTKGSCVPGPACAYVNLNICANGTEQGGVLLLENPKGDNQLNLVKLKTVVTS
  VFGVKNAKLSVFHGETEIQNQTDLLSLSGRTLCVTAGASPSPISSPSTLLCQYLNLQLLNAEPQECLTGT
  VGTLLLENPLGQNGLTHQGLVHEAAKVFGLRSRRLRLFLNETQTQEITEDIPMKTLNMKTVYVSVLPTTA
  DG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172015
• ORF Size: 3786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172015.3, NP_742012.2
• RefSeq Size: 4396 bp
• RefSeq ORF: 3789 bp
• Locus ID: 105148
• UniProt ID: Q8BU30
• Cytogenetics: 13 A5
• Gene Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.[UniProtKB/Swiss-Prot Function]