Brd8 (BC025644) Mouse Tagged ORF Clone

CAT#: MG211186

Brd8 (tGFP-tagged) - Mouse bromodomain containing 8 (cDNA clone MGC:36597 IMAGE:5323755)

Product Images

Circular map for MG211186

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Brd8
• Synonyms: SMAP, 4933408B17Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211186 representing BC025644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGTGGGGATCAGAACTGGGTATCAGTTAGCAGAGCAATCAAGCCCTTTGCAGAACCTGGCCGGC
  CTCCAGACTGGTTCTCTCAAAAACATTGTGCTTCTCAGTATTCTGAGCTCTTAGAGACTACTGAGACTCC
  AAAACGGAAACGGGGTGAAAAAGGAGAAGTGGTAGAAACTGTTGAAGATGTTATTGTTCGCAAACTGACT
  GCTGAGAGAGTTGAAGAACTGAAGAAAGTCATAAAGGAGACACAGGAGAGATACAGGCGTCTGAAAAGAG
  ATGCAGAACTAATCCAAGCCGGGCACATGGACAGCAGACTGGATGAGCTCTGCAATGACATTGCAATGAA
  AAAGAAATTGGAAGAGGAAGAGGCTGAAGTAAAAAGGAAGGCCACCGATGCTGCGTACCAGGCGCGACAA
  GCAGTGAAAACGCCTCCTCGAAGGTTACCGACTGTGATGGTCCGCTCTCCTGTAGACTCTGCCTCCCCAG
  GAGGTGATTACCCACTTGGAGACTTGACTCCAACCACTATGGAAGAGGCTACCTCTGGGGTAACCCCTGG
  GACTTTGCCGAGTACCCCAGTCACCTCGTTTCCTGGGATTCCTGACACCCTTCCTCCAGGCTCTGCACCC
  TTAGAAGCCCCCATGACCCCAATAACAGATGATTCACCCCAGAAAAAGATGCTTGGACAGAAAGCAACTC
  CACCCCCCTCCCCTCTGCTGTCAGAGCTCTTGAAGAAGGGCAGCCTCCTGCCTACTAGCCCCAGACTGGT
  GAATGAGAGTGAAATGCCTGTACCCCCTGGCCATCTGAACAGCACAGGGGTTCTCTTGGAGGTAGGAGGG
  GTTCTTCCCATGATACATGGTGGGGAGATACAGCCAACAACCAGTGCTGTGGCGGCCTCCCCGGCTGCCT
  CAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGCTGGGCCCACACAGTTCACCACTCCTCTTCCTTCCTT
  CACTACTGTTGCCAGTGAGCCGCCAGTTAAGCTTGTGCCACCCCCTGTAGAGTCTGTGTCCCAGGCTACC
  ATTGTCATGATGCCCGCGCTGCCAGCACCATCCTCTGCTGCGGCTGTCTCCACTTCTGAAAGTGGAGCTC
  CAGTGAGCCAGCCTGAGCCCTGTGTACCCCTGGAAGCTGTGGGGGATCCACATACTGTGACTGTTTCCAT
  GGATAGCAATGAAATCTCCATGATCATTAATTCCATCAAAGAAGAGTGTTTCCGCTCAGGGGTAGCAGAG
  GCTCCTGGTGGATCAAAGGCTCCAAGCATAGATGGGAAGGAAGACTTGGATCTAGCGGAGAAGATGGATA
  TTGCTGTGTCTTACACAGGTGAAGAGTTGGACTTTGAAACAGTTGGAGACATCATTGCCATCATTGAGGA
  CAAGGTGGATGATCACCCTGAAGTGCTGGATGTGGCAGCGGTAGAAGCGGCGTTATCGTTCTGTGAAGAG
  AATGATGACCCTCAGTCCCTGCCTGGCCCCTGGGAGCACCCTATCCAGCAGGAGCGCGACAAGCCAGTAC
  CTCTGCCAGCACCAGAGATGACAGTCAAACAAGAGAGGCTAGACTTCGAGGAATCAGAAAACAAAGGGCT
  CCATGACCTGGTGGACATCAGGGATTCCGGTGTTGAGATTAAGGTGGAACCCACAGAGCCAGAGCCAGGC
  ATGTCTGGGGCTGAGATAGTAGCTGGAGTTGGTCCAGTTCCAAGTATGGAGCCACCAGAACTCAGGAGTC
  AAGACTCAGATGAAGAACCTAGAAGTTCTGCAGCTGGAGACATTGGTGAGGCAGATGGTTCCAGTGGGAA
  AGGCGATGAGAGGCCACTTTCAGCTGTGAAGACAGAGGCATCCCCTGAGAGCATGTTGTCTCCATCACAT
  GGCTCAAATCTTATTGAAGATCCTTTAGAGGCAGAGACTCAACACAAGTTTGAAATGTCAGACTCATTGA
  AAGAAGAATCAGGGACTATTTTTGGAAGCCAGATAAAGGATGCCCCAGGTGACGATGAGGAAGAAGATGG
  AGTCAGTGAAGCAGCTAGCCTAGAGGAGCCTAAGGAAGAGGATCAAGGAGAAGGCTATTTGTCTGAGATG
  GATAATGAGCCCCCTGTGAGTGAGAGTGATGATGGCTTTAGTATACATAACGCCACACTGCAGTCACACA
  CTCTGGCAGACTCCATCCCAAGCAGCCCTGCCTCCTCCCAGTTTTCCGTGTGCAGTGAAGATCAAGAAGC
  AATTCAGGCTCAGAAAATATGGAAGAAAGCCATCATGCTTGTATGGAGGGCTGCAGCAAATCATAGGTAT
  GCCAATGTGTTCCTGCAACCTGTTACAGATGACATAGCTCCTGGTTACCATAGCATTGTACAGAGGCCTA
  TGGATTTGTCAACTATAAAGAAAAACATTGAAAATGGACTGATCCGAAGCACAGCTGAGTTTCAGCGTGA
  CATCATGCTGATGTTTCAGAATGCTGTTATGTACAATAGCTCAGACCATGATGTCTATCACATGGCAGTA
  GAGATGCAGAGAGATGTCTTGGAACAGATCCAGCAATTCTTGGCCACACAGTTGATTATGCAAACATCTG
  AGTCTGGAATCAGTGCTAAAAGTCTCCGGGGGAGAGACTCTACCCGAAAACAAGATGCTTCAGAGAAGGA
  CAGTGTCCCCATGGGCTCTCCTGCCTTCCTTCTCTCTCTCTTTGATGGGGGAACCAGGGGACGCCGCTGT
  GCCATTGAAGCAGATATGAAGATGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211186 representing BC025644
  Red=Cloning site Green=Tags(s)
  
  MRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSELLETTETPKRKRGEKGEVVETVEDVIVRKLT
  AERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDELCNDIAMKKKLEEEEAEVKRKATDAAYQARQ
  AVKTPPRRLPTVMVRSPVDSASPGGDYPLGDLTPTTMEEATSGVTPGTLPSTPVTSFPGIPDTLPPGSAP
  LEAPMTPITDDSPQKKMLGQKATPPPSPLLSELLKKGSLLPTSPRLVNESEMPVPPGHLNSTGVLLEVGG
  VLPMIHGGEIQPTTSAVAASPAASGAPTLSRLLEAGPTQFTTPLPSFTTVASEPPVKLVPPPVESVSQAT
  IVMMPALPAPSSAAAVSTSESGAPVSQPEPCVPLEAVGDPHTVTVSMDSNEISMIINSIKEECFRSGVAE
  APGGSKAPSIDGKEDLDLAEKMDIAVSYTGEELDFETVGDIIAIIEDKVDDHPEVLDVAAVEAALSFCEE
  NDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDFEESENKGLHDLVDIRDSGVEIKVEPTEPEPG
  MSGAEIVAGVGPVPSMEPPELRSQDSDEEPRSSAAGDIGEADGSSGKGDERPLSAVKTEASPESMLSPSH
  GSNLIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAPGDDEEEDGVSEAASLEEPKEEDQGEGYLSEM
  DNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFSVCSEDQEAIQAQKIWKKAIMLVWRAAANHRY
  ANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIRSTAEFQRDIMLMFQNAVMYNSSDHDVYHMAV
  EMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTRKQDASEKDSVPMGSPAFLLSLFDGGTRGRRC
  AIEADMKMKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC025644
• ORF Size: 2762 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025644, AAH25644
• RefSeq Size: 3277 bp
• RefSeq ORF: 2762 bp
• Locus ID: 78656
• Cytogenetics: 18 B1
• Gene Summary: May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.[UniProtKB/Swiss-Prot Function]