Gba2 (NM_172692) Mouse Tagged ORF Clone

CAT#: MG211175

Gba2 (tGFP-tagged) - Mouse glucosidase beta 2 (Gba2)

Product Images

Circular map for MG211175

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gba2
• Synonyms: F630034E04
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211175 representing NM_172692
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAACCTGCGTCCCGGCCTCAGAGCAGGTCGGCTGTGCCGAAAGAGATTCCCAAGTTTATTGTGAAG
  ATACTGGCGGCACTGAGGCTGTGCGGGTTACAGACTGCGGGAGCCCCGAGGATAGTGGACCCCAGGATGA
  ACCAAGCTACTGCAATTCAGAGGACTCTGGGCAGCTGATGGCCTCCTACGAGGGTAAAGCTAGGGGCTAC
  CAGGTGCCTCCTTTTGGCTGGCGGATCTGCTTGGCTCACGAGTTTGCAGAGAAGAGGAGACCTTTTCAAG
  CTAACAACATCTCTCTCAGTAATTTGGTAAAGCACCTCGGTATGGGCTTGAGGTACTTGAAGTGGTGGTA
  CCGAAAGACCCACGTGGAAAAGAAGACCCCTTTCATCGACATGCTCAATTCTCTACCCCTGAGACAGATC
  TATGGTTGTCCCCTGGGTGGCATTGGAGGAGGCACTATCACCCGGGGCTGGAGAGGCCAGTTCTGTCGTT
  GGCAGCTCAACCCTGGAATGTACCAGCACCAGACAGTCATTGCAGACCAATTTATAGTATGCTTGCGTCG
  AGATGGGCGGACTGTGTACCAGCAAGTTCTGTCCTTGGAGCTTCCAAATGTCCTGCGCAGCTGGAACTGG
  GGCCTGTGTGGTTACTTTGCTTTCTACCACGCCCTCTATCCCCGAGCCTGGACGGTCTATCAGCTTCCTG
  GCCAGAATGTCACCCTCACCTGTCGCCAGGTCACACCTATCTTGCCTCATGACTACCAGGACAGCAGTCT
  CCCTGTAGGAGTCTTTGTGTGGGATGTAGAAAACGAAGGAGATGAGACTCTGGACGTTTCCATCACGTTC
  TCCATGCGGAATGGACTAGGAGGCGAAGATGATGCGGCCGGGAGTTTGTGGAATGAGCCCTTCCGCCTGG
  AACAGGGCGGGACGACTGTTCAGGGGCTCCTCTTGCATCATCCAACCCCTCCGAACCCCTACACCATGGC
  TGTGGCTGCACGATGCACGGCAGATACCACGGTAACCCACACCACAGCCTTTGACCCCAATGGCACTGGA
  CAGCAGGTGTGGCAGGACCTACTTCAGGATGGACAGCTGGACTCCCCTGCTGGCCAAAGCACCCCAACAC
  AGAAAGGAGAGGGTATCGCTGGGGCTGTATGCGTCTCTAGCAAGCTGTTACCTCGAAGCCGGTGCTGCTT
  GGAGTTCTCACTGGCTTGGGATATGCCTAAGATCATGTTTGGAGCTAAAAGCCAAGTCCACTACAGGCGG
  TATACACGGTTCTTTGGTTCAGATGGTGATGTGGCGCCTGCCCTGAGCCACTATGCACTGTGCCACTATG
  CAGACTGGGAGGACAGAATCTCAGCCTGGCAGAACCCAGTACTGGATGACAGAACCTTGCCTGCCTGGTA
  CAAATCTGCACTGTTCAATGAACTGTACTTCCTGGCCGATGGAGGCACAGTGTGGCTGGAAGTTCCCGCA
  GACTCCCTACCAGAGGGGCTGGGAGGGAGTATGCGTCAGCTTCGCTCTACTCTGCAGGACTATGGGCGAT
  TTGGCTATCTTGAAGGCCAGGAGTACCGCATGTACAACACATACGATGTCCACTTTTATGCGTCCTTTGC
  CCTCGTCATGCTGTGGCCCAAACTTGAGCTCAGTCTTCAGTATGATATGGCTCTGGCAACTTTGAAGGAG
  GACCTGACCCGGCGACGGTACCTGATGAGTGGAGTGGTGGCACCTGTGAAAAGGAGGAACGTCATCCCTC
  ATGACATTGGGGATCCGGATGATGAGCCATGGCTCCGGGTCAACGCATATTTGATTCATGATACTGCTGA
  CTGGAAGGACCTGAACCTGAAGTTTGTATTGCAAATTTATCGGGACTATTACCTGACGGGTGATCAAGGC
  TTCCTGGAGGACATGTGGCCTGTGTGTCTGGCTGTGATGGAGTCCGAAATGAAGTTTGACAAGGACCAAG
  ATGGACTCATTGAGAATGGAGGCTACGCAGACCAGACCTATGATGCATGGGTCACCACAGGCCCCAGTGC
  TTACTGCGGAGGGCTGTGGCTGGCGGCAGTGGCTGTAATGGTTCAGATGGCTGTTCTGTGTGGGGCCCAA
  GATGTCCAGGAGAGGTTTGCTTCCATTCTCTGCCGAGGCCGAGAAGCTTATGAGAGACTGCTGTGGAACG
  GACGCTATTACAACTACGACAGCAGCTCCCATCCTCAGTCTCGGAGCATCATGTCTGACCAGTGTGCTGG
  GCAGTGGTTCCTGAGGGCCTGCGGCCTGGGAGAAGGAGACACTGAAGTATTTCCTACCCTGCATGTGGTC
  CGTGCTCTCCAAACCATCTTTGAACTCAACGTCCAAGCCTTTGCAGGAGGAGCCATGGGGGCCGTGAACG
  GGATGCACCCTCATGGTGTCCCTGATAGATCCAGTGTGCAGTCTGATGAAGTCTGGGTGGGTGTGGTCTA
  TGGGCTGGCAGCCACCATGATCCAAGAGGGCCTGACTTGGGAAGGTTTCCGGACAGCTGAAGGCTGTTAC
  CGCACTGTATGGGAACGCCTGGGCCTGGCTTTCCAGACCCCAGAGGCATACTGCCAGCAACAAGTGTTCC
  GCTCCCTGGCCTACATGCGGCCACTGAGCATCTGGGCCATGCAGCTGGCCCTGCAACAGCAGCAGCATAA
  AAAGAGCCGCAGGCCATCAGTCACACAAGGCACGGGACTAAGCACACAGCCTGAATGTGGACCAAAGAGA
  TCGCTGGCAAACCTCAATTCAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211175 representing NM_172692
  Red=Cloning site Green=Tags(s)
  
  MVTCVPASEQVGCAERDSQVYCEDTGGTEAVRVTDCGSPEDSGPQDEPSYCNSEDSGQLMASYEGKARGY
  QVPPFGWRICLAHEFAEKRRPFQANNISLSNLVKHLGMGLRYLKWWYRKTHVEKKTPFIDMLNSLPLRQI
  YGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPNVLRSWNW
  GLCGYFAFYHALYPRAWTVYQLPGQNVTLTCRQVTPILPHDYQDSSLPVGVFVWDVENEGDETLDVSITF
  SMRNGLGGEDDAAGSLWNEPFRLEQGGTTVQGLLLHHPTPPNPYTMAVAARCTADTTVTHTTAFDPNGTG
  QQVWQDLLQDGQLDSPAGQSTPTQKGEGIAGAVCVSSKLLPRSRCCLEFSLAWDMPKIMFGAKSQVHYRR
  YTRFFGSDGDVAPALSHYALCHYADWEDRISAWQNPVLDDRTLPAWYKSALFNELYFLADGGTVWLEVPA
  DSLPEGLGGSMRQLRSTLQDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATLKE
  DLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQIYRDYYLTGDQG
  FLEDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDAWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQ
  DVQERFASILCRGREAYERLLWNGRYYNYDSSSHPQSRSIMSDQCAGQWFLRACGLGEGDTEVFPTLHVV
  RALQTIFELNVQAFAGGAMGAVNGMHPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEGCY
  RTVWERLGLAFQTPEAYCQQQVFRSLAYMRPLSIWAMQLALQQQQHKKSRRPSVTQGTGLSTQPECGPKR
  SLANLNSE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172692
• ORF Size: 2754 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172692.3, NP_766280.2
• RefSeq Size: 3552 bp
• RefSeq ORF: 2757 bp
• Locus ID: 230101
• UniProt ID: Q69ZF3
• Cytogenetics: 4 A5
• Gene Summary: Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide (PubMed:17080196, PubMed:23250757). Glucosylceramides are membrane glycosphingolipids that have a wide intracellular distribution (PubMed:23250757). They are the main precursors of more complex glycosphingolipids that play a role in cellular growth, differentiation, adhesion, signaling, cytoskeletal dynamics and membrane properties (PubMed:25803043). Also involved in the transglucosylation of cholesterol, transferring glucose from glucosylceramides, thereby modifying its water solubility and biological properties (PubMed:26724485). Under specific conditions, may catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485). Finally, may also play a role in the metabolism of bile acids (PubMed:17080196). It is able to hydrolyze bile acid 3-O-glucosides but also to produce bile acid-glucose conjugates thanks to a bile acid glucosyl transferase activity (PubMed:17080196). However, the relevance of both activities is unclear in vivo (PubMed:17080196).[UniProtKB/Swiss-Prot Function]