Rps6ka4 (NM_019924) Mouse Tagged ORF Clone
CAT#: MG210573
- TrueORF®
Rps6ka4 (tGFP-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 4 (Rps6ka4)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_019924" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Rps6ka4 |
Synonyms | 90kDa; 1110069D02Rik; AI848992; mMSK2; Msk2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210573 representing NM_019924
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACGAGGATGAGGACGAGGGCTGCGCCGTGGAGCTGCAGATCACCGAAGCCAACCTCACCGGGC ATGAGGAGAAGGTGAGCGTGGAGAACTTCGCGCTGCTCAAGGTGCTGGGCACGGGAGCCTATGGGAAGGT GTTCCTGGTGCGGAAGACGGGTGGGCACGACGCGGGCAAGCTCTATGCCATGAAGGTGCTACGCAAGGCG GCGTTGGTGCAGCGCGCGAAGACACAGGAGCATACCCGCACCGAACGCTCGGTGCTGGAGCTGGTTCGCC AAGCACCCTTCCTGGTCACACTGCACTACGCCTTCCAGACGGATGCCAAGCTGCACCTCATCCTGGACTA CGTGAGCGGTGGTGAGATGTTCACTCACCTCTACCAGCGCCAGTACTTCAAGGAGGCTGAGGTTCGAGTG TATGGGGGAGAGATTGTGCTGGCCCTGGAACACCTGCACAAGCTGGGTATCATCTACCGGGACCTGAAGC TGGAGAACGTCTTACTTGACTCAGAAGGTCACATCGTCCTTACAGACTTTGGGCTGAGCAAGGAGTTCCT GACGGAGGAGAAAGAGCGGACCTTCTCCTTCTGTGGCACAATCGAGTACATGGCTCCCGAAATCATCCGA AGCAAGGCTGGACATGGCAAGGCTGTGGACTGGTGGAGCCTGGGTATCCTGCTCTTCGAGCTGCTGACAG GAGCCTCACCCTTCACACTGGAGGGAGAGAGGAACACTCAGGCTGAGGTGTCCCGACGGATCTTGAAGTG CTCCCCTCCCTTCCCTCTCCGGATTGGGCCTGTGGCACAGGACCTGCTACAGCGGCTGCTGTGCAAGGAC CCTAAGAAGAGGTTGGGCGCAGGTCCCCAGGGTGCGCAGGAAGTCAAGAGTCACCCCTTCTTCCAGGGTC TGGACTGGGTGGCTCTGGCTGCCAGAAAGATCCCAGCCCCATTCCGGCCCCAGATCCGCTCAGAGCTGGA TGTGGGGAATTTTGCGGAGGAATTCACCCGGCTGGAGCCCGTCTACCCCCCTGCCGGCAGCCCTCCACCT GGGGACCCTCGGATCTTTCAGGGATACTCCTTCGTGGCTCCGTCCATCCTCTTTGACCACAACAATGCAG TGATGGCTGATGTACTGCAGGCACCCGGTGCTGGATACAGGCCGGGCAGGGCAGCAGTTGCCAGGAGTGC CATGATGCAGGACTCGCCTTTCTTCCAGCAGTACGAACTGGACCTTCGGGAACCAGCACTGGGGCAGGGC AGCTTCTCTGTGTGTCGGAGATGTCGGCAGCGCCAGAGCGGCCAGGAGTTTGCTGTCAAGATCCTCAGCC GCAGGCTGGAGGAGAACACTCAGAGAGAGGTGGCTGCTCTTCGCCTGTGCCAGTCACACCCCAACGTGGT GAATCTGCATGAGGTGCTTCATGACCAGCTACACACTTACCTGGTCCTGGAGTTGCTGCGAGGCGGAGAG CTATTGGAACACATCCGCAAGAAGCGGCTCTTCAGCGAGTCCGAGGCCAGCCAGATCCTTCGGAGCCTGG TTTCGGCCGTGAGCTTCATGCACGAGGAGGCAGGCGTGGTGCACCGCGACCTGAAACCCGAGAACATCTT GTACGCGGACGACACTCCCGGGGCCCCGGTGAAGATCATCGACTTCGGGTTCGCGCGACTGCGGCCCCAG AGCCCGGCAGAGCCCATGCAGACTCCTTGCTTCACACTGCAGTACGCTGCACCCGAGCTGCTGGCACAGC AGGGCTACGATGAGTCCTGCGATCTATGGAGCCTGGGTGTCATTCTGTACATGATGCTGTCTGGCCAGGT TCCCTTCCAAGGGGCCTCGGGCCAGGGTGGACAGAGTCAGGCAGCTGAGATCATGTGCAAGATCCGTGAA GGGCGCTTCTCCCTGGACGGGGAAGCCTGGCAAGGTGTGTCGGAGGAAGCCAAGGAGCTGGTCCGAGGGC TACTGACAGTCGACCCCGCCAAGCGGCTGAAGCTGGAGGGGCTGCGTAGCAGCTCGTGGCTTCAGGACGG CAGCGCGCGCTCCTCGCCCCCGCTCCGCACGCCGGATGTGCTGGAGTCCTCTGGGCCAGCTGTGCGTTCC GGGCTCAATGCCACTTTCATGGCGTTCAACCGAGGCAAGCGCGAGGGCTTCTTTCTCAAGAGTGTAGAGA ATGCGCCTCTGGCCAAGAGGCGCAAGCAGAAGCTCCGGAGCGCCGCCGCCTCCCGTCGCGGCTCCCCAGT GCCTGCCTCCTCCGGTCGCCTACCAGCCTCTGCCGCTAAGGGGACAACTCGCCGAGCCAACGGCCCCTTG TCCCCTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210573 representing NM_019924
Red=Cloning site Green=Tags(s) MGDEDEDEGCAVELQITEANLTGHEEKVSVENFALLKVLGTGAYGKVFLVRKTGGHDAGKLYAMKVLRKA ALVQRAKTQEHTRTERSVLELVRQAPFLVTLHYAFQTDAKLHLILDYVSGGEMFTHLYQRQYFKEAEVRV YGGEIVLALEHLHKLGIIYRDLKLENVLLDSEGHIVLTDFGLSKEFLTEEKERTFSFCGTIEYMAPEIIR SKAGHGKAVDWWSLGILLFELLTGASPFTLEGERNTQAEVSRRILKCSPPFPLRIGPVAQDLLQRLLCKD PKKRLGAGPQGAQEVKSHPFFQGLDWVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYPPAGSPPP GDPRIFQGYSFVAPSILFDHNNAVMADVLQAPGAGYRPGRAAVARSAMMQDSPFFQQYELDLREPALGQG SFSVCRRCRQRQSGQEFAVKILSRRLEENTQREVAALRLCQSHPNVVNLHEVLHDQLHTYLVLELLRGGE LLEHIRKKRLFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQ SPAEPMQTPCFTLQYAAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIRE GRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRSSSWLQDGSARSSPPLRTPDVLESSGPAVRS GLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSAAASRRGSPVPASSGRLPASAAKGTTRRANGPL SPS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_019924 |
ORF Size | 2319 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019924.1, NP_064308.1 |
RefSeq Size | 3120 bp |
RefSeq ORF | 2322 bp |
Locus ID | 56613 |
UniProt ID | Q9Z2B9 |
Cytogenetics | 19 A |
Gene Summary | Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200919 | Rps6ka4 (untagged) - Mouse ribosomal protein S6 kinase, polypeptide 4 (Rps6ka4), (10ug) |
USD 709.00 |
|
MR210573 | Rps6ka4 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 4 (Rps6ka4) |
USD 709.00 |
|
MR210573L3 | Lenti ORF clone of Rps6ka4 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 4 (Rps6ka4) |
USD 1,009.00 |
|
MR210573L4 | Lenti ORF clone of Rps6ka4 (mGFP-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 4 (Rps6ka4) |
USD 1,009.00 |
{0} Product Review(s)
Be the first one to submit a review