Tmem2 (BC019745) Mouse Tagged ORF Clone

CAT#: MG210404

Tmem2 (tGFP-tagged) - Mouse transmembrane protein 2 (cDNA clone MGC:30634 IMAGE:3675225)

Product Images

Circular map for MG210404

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tmem2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210404 representing BC019745
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGATGGAGTGTTTGGAAACTACGTCCCTGTGCCTACCACAGATTGCATGGCTGTTTCAACTTTCT
  GGATTGCCCACCCCAACAACCACCTGATCAATAACGCAGCTGCAGGCTCACAGGATGCTGGAATATGGTA
  TTTATTCCACAAGGAACCAACTGGGGAGTCCAGTGGGCTGCAGCTCCTGGAGAAGCCAGAACTCACTCCG
  CTGGGGATATTTTATAACAACAGGGTTCATTCAAATTTTAAGGCTGGTTTATTTGTGGACAAAGGTGTCA
  AAACGACCAACGCCAGTGCATCTGACCCAAGGGAGTACCTCTGCCTGGATAACAGTGCAAGGTTTCGGCC
  CCATCAGGATGCAGACCCTGAGAAGCCACGCGTTGCCGCCATCATTGACAGGCTCATTGCTTTTAAGAAC
  AATGACAATGGAGCCTGGGTCCGAGGAGGAGATATTATTGTTCAGAATTCAGCATTTGCAGATAACGGAA
  AGGGGCTGACCTTTGCCAGTGATGGGAGCTTCCCAAGCGATGAAGGGTCCAGTCAGGAGGTGACGGAGTC
  CCTCTTTGTCGGGGAGAGTAGGAATTATGGCTTTCAAGGTGGCCAAAACAAGTACATGGGCACAGGGGGA
  ATCGATCAGAAGCCTCGGACGCTACCCAGGAACAGGACATTCCCAATCCGAGGCTTTCAGATTTACGATG
  GGCCTATTCATCTCACAAAGAGTACTTTCAAAAAGTATGTGCCAACTCCAGACAGGTACAGCAGTGCAAT
  TGGCTTCCTCATGAAGAATTCCTGGCAGACAACACCCAGGAATAACGTTTCCCTTGTGAAGTTTGGTCCA
  CAGGTCTCTCTGAACGTCTTCTTTGGAAAGCCTGGCCCCTGGTTTGAGGATTGTGAGCTGGACGGCGATA
  AGAACTCCATCTTCCATGACATTGATGGCTCTGTGACAGGATACAAGGACACCTATGTGGGAAGGATGGA
  CAACTACCTGATCCGCCATCCAAACTGTGTCAATGTTACTAAGTGGAATGCAGTGATCTGTAGCGGGACC
  TACGCCCAGGTGTATGTTCAGACCTGGAACACTCCGAATCTTAGCATGATCATCACACGGGATGAATACC
  CATCCCACCCAATGGTGCTTCGGGGTATCAACCAGAGGGCCATCTCTCCACAGTACCAGCCTGTCGTCAT
  GCTGGAGAAGGGCTACACCATCCACTGGAATGGTCCAGCGCCACGAACCACTTTCTTGTACCTCGTGAAC
  TTCAACAAGGATGACTGGATCCGGGTTGGCCTCTGTTACCCAGCCAACACGAGCTTTCAGGTTACCGTTG
  GCTTTCTGCAGCGGCAGAACGGCTCATTATCTAGAATTGAGGACTATGAGCCTGCGCGGTCGATGGAGGA
  ACTGCAGAAGAAGCCGTCTGAGAGAAAATTCTATTTTGACTCTGGCACAGGGTTATTGTTTCTGTATCTC
  AGAGCCCACAGCCACAGAGATGGCCACAGTTACTGTTCATCTCAGGGATGTGAAAGAGTCAAGATTCAGG
  CAGCAACAGACTCCAAAGATATCAGTAACTGCATGGCCAAAGCTTACCCGCAGTATTACAAGAAGCCCTC
  GGCGGTCAAGCGCATGCCGGCCATGCTCACGGGGCTCTGTCAAGGCTGTGGAACCCACCAGATGGTGTTT
  ACCAGTGACCCTCACAAAAGCTACCTCCCTGTACGATTCCAGTCACCTGGGAAAGCAGAAATTCAGCGAG
  GAGATCCATCCATTATTTCCGTCAATGGTACTGACTTTACCTTCCGGAGTGCGGGTGTCCTCCTCCTCGT
  TGTGGATGCCTGCAGTGTGCCTTTCCGGGTGAAGGAAAAGCGGATGTTCCTTTCTGCAGACGTCAGTCAC
  ATGGAAGAATATTTCAAGGCTAGCATCCCCCCAAGGTCAATCGTTCTGTTGAGCACAAGAGGAGAAATTA
  AGCAGCTGAATATCTCGGATTCATTAGCACTTCTGGGATTGGCCAAGCCAGCACATCTGTACAGCAAAGG
  TAGTGTCGTATTTTTGGGATTCAGCGGAAACTTCGCACCGTCATGGACGAAGCTATTCACCAGTCCCAAT
  GAGCAGGGCCTCGGAGTGCTTGAGCAGTTTCTCCCGTTGCAGATGGAGGAATATGGTTGCTCCCGGACCG
  GCAGTGTCCACAGAAGAGACCTTGACCTGTTACAGCAGGCTTTGAAAGTGCTT
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210404 representing BC019745
  Red=Cloning site Green=Tags(s)
  
  MRDGVFGNYVPVPTTDCMAVSTFWIAHPNNHLINNAAAGSQDAGIWYLFHKEPTGESSGLQLLEKPELTP
  LGIFYNNRVHSNFKAGLFVDKGVKTTNASASDPREYLCLDNSARFRPHQDADPEKPRVAAIIDRLIAFKN
  NDNGAWVRGGDIIVQNSAFADNGKGLTFASDGSFPSDEGSSQEVTESLFVGESRNYGFQGGQNKYMGTGG
  IDQKPRTLPRNRTFPIRGFQIYDGPIHLTKSTFKKYVPTPDRYSSAIGFLMKNSWQTTPRNNVSLVKFGP
  QVSLNVFFGKPGPWFEDCELDGDKNSIFHDIDGSVTGYKDTYVGRMDNYLIRHPNCVNVTKWNAVICSGT
  YAQVYVQTWNTPNLSMIITRDEYPSHPMVLRGINQRAISPQYQPVVMLEKGYTIHWNGPAPRTTFLYLVN
  FNKDDWIRVGLCYPANTSFQVTVGFLQRQNGSLSRIEDYEPARSMEELQKKPSERKFYFDSGTGLLFLYL
  RAHSHRDGHSYCSSQGCERVKIQAATDSKDISNCMAKAYPQYYKKPSAVKRMPAMLTGLCQGCGTHQMVF
  TSDPHKSYLPVRFQSPGKAEIQRGDPSIISVNGTDFTFRSAGVLLLVVDACSVPFRVKEKRMFLSADVSH
  MEEYFKASIPPRSIVLLSTRGEIKQLNISDSLALLGLAKPAHLYSKGSVVFLGFSGNFAPSWTKLFTSPN
  EQGLGVLEQFLPLQMEEYGCSRTGSVHRRDLDLLQQALKVL
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: BC019745
• ORF Size: 2225 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC019745, AAH19745
• RefSeq Size: 2374 bp
• RefSeq ORF: 2225 bp
• Locus ID: 83921
• Cytogenetics: 19 B
• Gene Summary: Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 5 kDa fragments (By similarity). Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity). Is very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate (By similarity).[UniProtKB/Swiss-Prot Function]