Mcm7 (NM_008568) Mouse Tagged ORF Clone
CAT#: MG210265
- TrueORF®
Mcm7 (tGFP-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_008568" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Mcm7 |
Synonyms | AI747533; mCDC47; Mcmd7 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210265 representing NM_008568
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTTAAGGACTACGCGATCGAAAAAGAAAAAGTTAAGAAGTTCCTCCAAGAGTTTTATTACGAGA ATGAGCTTGGAAAGAAGCAGTTCAAGTATGGGACCCAGTTGGTTCATCTGGCTCATCGGGAACAAGTGGC ACTGTATGTGGACCTGGATGACATAGCTGAAGATGACCCTGAGTTGGTCGACTCAATTTGCGAGAATGCC AAGCGCTACTCAAGACTGTTTGGTGATGTTGTGCAAGAGCTTTTGCCTGAGTACAAGGAGAAGGAGGTGG TGAATAAGGATGTCTTAGATGTTTACATTGAACACCGGCTGATGATGGAGCAGCGAAGCAGAGACCCTGG GGCAGTCCGGAACCCCCAAAACCAGTATCCTTCTGAACTCATGCGAAGATTTGAGTTGTATTTCCGAGGC CCAAGTAGCAGCAAGCCTCGAGTGATCCGGGAAGTACGAGCTGACTCTGTGGGGAAATTGTTAACTGTGC GTGGCATTGTCACTCGTGTGTCTGAAGTCAAGCCCAGGATGGTGGTGGCCACATACACTTGTGATCAGTG TGGGGCAGAGACCTACCAGCCAATCCAGTCTCCCACTTTCATGCCTCTGATCATGTGCCCCAGCCAGGAG TGCCAGACCAATCGCTCAGGGGGGCGGCTGTACCTACAGACTCGTGGCTCCAAATTTGTTAAATTCCAGG AAATGAAGATTCAAGAGCATAGTGACCAAGTTCCTGTGGGAAACATCCCTCGTAGCATCACAGTGGTTCT AGAAGGTGAGAACACAAGAATTGCCCAGCCTGGTGACCATGTCAGCGTCACTGGCATCTTCTTGCCAGTC CTGCGCACAGGGTTCCAACAGATGGCACAGGGCTTACTCTCAGAAACTTACCTGGAAGCCCACTGGATTG TGAAGATGACAAAGAGTGACGATGATGTCTCTGGGGCCGGAGAGCTGAGCTCAGAGGAGCTGAAGCAGAT TGCAGAGGAGGATTTCTATGAGAAGCTGGCCGCTTCTATTGCTCCGGAGATCTATGGACATGAAGATGTG AAGAAGGCGCTGCTGCTGCTTCTGGTGGGTGGTGTGGACCAGTCTCCTCAAGGCATGAAGATCAGAGGAA ACATCCACATCTGCCTTATGGGAGACCCTGGTGTGGCCAAATCTCAGCTCCTATCTTACATCGACCGTCT GGCACCGCGAAGTCAGTACACAACAGGCCGTGGCTCCTCTGGTGTAGGGCTCACAGCAGCTGTGTTGAGA GACTCTGTGAGTGGAGAACTGACTTTAGAGGGCGGTGCCCTGGTGCTGGCTGACCAGGGCGTGTGCTGCA TTGATGAGTTTGACAAGATGGCCGAGGCTGACCGCACAGCCATCCATGAGGTTATGGAGCAGCAGACCAT CTCCATCGCCAAGGCGGGGATTCTCACCACGCTCAATGCCCGATGCTCCATCCTGGCTGCGGCCAATCCT GCCTACGGCCGATATAATCCCCGCCGCAGTCTGGAGCAGAACGTCCAGCTTCCGGCTGCTCTGCTTTCTC GATTCGACCTCCTCTGGCTGATTCAAGACCGGCCCGACAGAGACAATGACCTCCGGTTGGCCCAGCACAT CACCTATGTCCACCAGCACAGCCGGCAGCCCCCTGCCCAATTTGAACCTTTGGACATGAAACTTATGAGA CGGTACATAGCCATGTGCCACGAGAGACAGCCCACAGTGCCTGAATCTCTGGCTGACTACATCACCGCAG CATATGTGGAGATGAGGCGAGAGGCCCGGGCCAGTAAGGATGCCACCTATACTTCTGCCCGGACCCTGCT GGCCATTCTCCGACTTTCCACTGCTCTGGCTCGACTTCGGATGGTAGACATTGTGGAGAAGGAAGATGTA AATGAAGCCATAAGGTTGATGGAGATGTCGAAGGACTCCCTTCTAGGTGAAAAGGGACAGACAGCTAGGA CCCAGAGGCCAGCAGATGTGATATTTGCCACCATCCGTGAATTGGTGTCACGGGGGCGGAGTGTCCACTT TTCTGAGGCTGAACAGCGCTGCATATCTCGTGGCTTCACACCAGCCCAGTTCCAGGCAGCTCTGGATGAA TATGAGGAGCTAAATGTCTGGCAGGTTAATACTTCTCGGACACGGATCACCTTTGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210265 representing NM_008568
Red=Cloning site Green=Tags(s) MALKDYAIEKEKVKKFLQEFYYENELGKKQFKYGTQLVHLAHREQVALYVDLDDIAEDDPELVDSICENA KRYSRLFGDVVQELLPEYKEKEVVNKDVLDVYIEHRLMMEQRSRDPGAVRNPQNQYPSELMRRFELYFRG PSSSKPRVIREVRADSVGKLLTVRGIVTRVSEVKPRMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQE CQTNRSGGRLYLQTRGSKFVKFQEMKIQEHSDQVPVGNIPRSITVVLEGENTRIAQPGDHVSVTGIFLPV LRTGFQQMAQGLLSETYLEAHWIVKMTKSDDDVSGAGELSSEELKQIAEEDFYEKLAASIAPEIYGHEDV KKALLLLLVGGVDQSPQGMKIRGNIHICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLR DSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNARCSILAAANP AYGRYNPRRSLEQNVQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPAQFEPLDMKLMR RYIAMCHERQPTVPESLADYITAAYVEMRREARASKDATYTSARTLLAILRLSTALARLRMVDIVEKEDV NEAIRLMEMSKDSLLGEKGQTARTQRPADVIFATIRELVSRGRSVHFSEAEQRCISRGFTPAQFQAALDE YEELNVWQVNTSRTRITFV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008568 |
ORF Size | 2157 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008568.3 |
RefSeq Size | 2396 bp |
RefSeq ORF | 2160 bp |
Locus ID | 17220 |
UniProt ID | Q61881 |
Cytogenetics | 5 G2 |
Gene Summary | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205195 | Mcm7 (untagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7), (10ug) |
USD 659.00 |
|
MR210265 | Mcm7 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7) |
USD 659.00 |
|
MR210265L3 | Lenti ORF clone of Mcm7 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7) |
USD 959.00 |
|
MR210265L4 | Lenti ORF clone of Mcm7 (mGFP-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7) |
USD 959.00 |
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