Rars (NM_025936) Mouse Tagged ORF Clone

CAT#: MG209847

Rars (tGFP-tagged) - Mouse arginyl-tRNA synthetase (Rars)

Product Images

Circular map for MG209847

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rars
• Synonyms: 2610011N19Rik; 2610037E21Rik; AL033339; AW985894
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209847 representing NM_025936
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGTCTGGTGGCTCAGTGCTCCGCGCGGTTGCTGCAGCAGGAAAGGGAGATTAAGGCTCTGACTG
  CTGAGATCGACCGGCTGAAGAACTGTGGCTGCTTGGAAGCTTCTCCAAGTTTGGAACAGTTACGAGAAGA
  AAATTTAAAATTGAAGTATAGGCTGAATATTCTTCGAAGGAGTCTTCAGGAAGAAAGGAGAAAACCAACT
  AAAAACATGATTAACATCAACAGCCGCCTGCAGGAAGTCTTTGGCTGTGCCATTAGGGCTGCATATCCAG
  ACTTGGAGAACCCTCCCCTGATCGTGACACCAAGTCAGCAGCCCAAGTTTGGAGACTATCAGTGTAATAG
  TGCCATGGGTATTTCTCAGATGCTCAAGGCAAAGGAACAGAAAGTCAGTCCCAGAGAAATCGCAGAAAAC
  ATTACCAAACACCTGCCGAACAATAAATATATTGACAAAGTTGAAATTGCGGGTCCTGGTTTCATTAATG
  TGCACCTGAGAAAAGACTTTGTGTCAGAACAGCTAACCAGTCTCCTAGTGAATGGAGTCCAGCTGCCTGT
  GCTTGGAGACAAGGAGAAGGTTATAGTTGACTTTTCCTCTCCCAACATAGCTAAGGAGATGCATGTTGGC
  CACCTCAGGTCAACCATCATAGGAGAGAGCATGAGCCGCCTCTTTGAATTTGCAGGATACGATGTGCTAA
  GGTTAAACCATGTAGGAGATTGGGGAACCCAGTTTGGCATGCTCATCGCTCACCTGCAAGATAAATTTCC
  GGATTATCTGACAGTTTCACCTCCTATTGGGGATCTGCAGGCCTTTTATAAGGAATCTAAGAAGAGGTTT
  GATGCTGATGAGGAGTTTAAGAAGCGAGCCTATCAGTGTGTCGTTTTGCTGCAGAGTAAAAACCCAGATA
  TCATGAAAGCCTGGAACCTCATCTGTGACGTGTCCCGGGAAGAGTTTAAGAAAATCTATGATGCATTGGA
  CATCACTTTAATAGAGAGAGGAGAATCCTTCTATCAAGATAGGATGAAGGACATTGTAAAGGAATTTGAA
  GATAAAGGGTTTGTGCAAGTGGATGATGGCAGAAAAATTGTGTTCGTGCCTGGGTGTTCTGTGCCATTAA
  CGATAGTGAAATCAGATGGGGGCTATACCTATGACACATCTGACCTGGCTGCCATCAAACAGAGACTGTT
  TGAAGAAAAAGCAAACAAGATTATTTATGTGGTGGACAATGGACAAGCTATACACTTCCAGACAATATTT
  GCTGCTGCTCAGATGATTGGCTGGTATGACCCGAAAGTAACTCTAGTGACCCACGTGGGATTTGGTGTCG
  TCCTAGGAGAAGACAAGAAGAAATTTAAAACACGTTCAGGTGAAACTGTGCGCCTTATGGACCTGCTGGA
  AGAAGGGCTGAAACGGTCCATGGACAAGCTAAAGGAGAAAGAGAGAGACAAGGTCTTAACTGAAGAGGAA
  TTGAAAGCAGCTCAGACATCCGTTGCATATGGCTGCATCAAATACGCCGACCTTTCTCATAATCGCCTCA
  ATGACTACATCTTCTCCTTTGACAAGATGCTGGATGACAGAGGGAACACAGCTGCTTACCTACTGTATGC
  CTTCACCAGAATCAGGTCTATTGCGCGCCTCGCCAACATTGATGAGGCGATGCTACAGAGAGCCGCTCGG
  GAAACCAAGATCATTTTGGACCATGAAAAGGAGTGGAAGCTGGGGCGGTGCATCTTACGGTTCCCCGAGA
  TCCTCCAGAAGATCTTAGACGACCTGTTTCTCCACACCCTCTGTGACTACATTTACGAGCTGGCAACCAC
  CTTCACAGAGTTCTACGATAGCTGCTACTGTGTGGAGAAAGATCGACAGACTGGGAAAGTATTGAAAGTG
  AACATGTGGCGGATGCTCCTGTGTGAGGCCGTGGCTGCTGTCATGGCCAAGGGGTTCGATATACTGGGGA
  TAAAACCCGTCCAAAGGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209847 representing NM_025936
  Red=Cloning site Green=Tags(s)
  
  MDGLVAQCSARLLQQEREIKALTAEIDRLKNCGCLEASPSLEQLREENLKLKYRLNILRRSLQEERRKPT
  KNMININSRLQEVFGCAIRAAYPDLENPPLIVTPSQQPKFGDYQCNSAMGISQMLKAKEQKVSPREIAEN
  ITKHLPNNKYIDKVEIAGPGFINVHLRKDFVSEQLTSLLVNGVQLPVLGDKEKVIVDFSSPNIAKEMHVG
  HLRSTIIGESMSRLFEFAGYDVLRLNHVGDWGTQFGMLIAHLQDKFPDYLTVSPPIGDLQAFYKESKKRF
  DADEEFKKRAYQCVVLLQSKNPDIMKAWNLICDVSREEFKKIYDALDITLIERGESFYQDRMKDIVKEFE
  DKGFVQVDDGRKIVFVPGCSVPLTIVKSDGGYTYDTSDLAAIKQRLFEEKANKIIYVVDNGQAIHFQTIF
  AAAQMIGWYDPKVTLVTHVGFGVVLGEDKKKFKTRSGETVRLMDLLEEGLKRSMDKLKEKERDKVLTEEE
  LKAAQTSVAYGCIKYADLSHNRLNDYIFSFDKMLDDRGNTAAYLLYAFTRIRSIARLANIDEAMLQRAAR
  ETKIILDHEKEWKLGRCILRFPEILQKILDDLFLHTLCDYIYELATTFTEFYDSCYCVEKDRQTGKVLKV
  NMWRMLLCEAVAAVMAKGFDILGIKPVQRM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025936
• ORF Size: 1980 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025936.3, NP_080212.2
• RefSeq Size: 2140 bp
• RefSeq ORF: 1983 bp
• Locus ID: 104458
• UniProt ID: Q9D0I9
• Cytogenetics: 11 A4
• Gene Summary: Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis (PubMed:12060739). Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.[UniProtKB/Swiss-Prot Function]