Fbxo21 (NM_145564) Mouse Tagged ORF Clone

CAT#: MG209562

Fbxo21 (tGFP-tagged) - Mouse F-box protein 21 (Fbxo21)

Product Images

Circular map for MG209562

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fbxo21
• Synonyms: 2810425J22Rik; AU016673
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209562 representing NM_145564
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGGTAGCGGGGGACAGCGCTATGGAGGTGGTGCCGGCGCTGGCCGAGGAGGCCGCGGCTGAGG
  CGACGGGCCCCAGCTGCCTGGTGCAGCTGCCGGGCGAGGTGCTGGAGTACATCCTGTGCAGCGGCTCGCT
  CACGGCGCTGGACATCGGCCGCGTGTCCAGCACCTGCCGCCGCCTGCGCGAGGTGTGCCAGAGCAGCGGG
  CAGGTGTGGAAGGAGCAGTTCCGGGTGAGGTGGCCCTCCCTGATGAAGCACTACAGCCCCACCGACTACG
  TCAATTGGTTAGAGGAATATAAAGTTCGGCAGAAAGCTGGGTTAGAAGCCCGGAAGATTGTAGCTTCCTT
  CTCCAAGCGGTTCTTTTCAGAGCACGTCCCCTGTAATGGCTTCAGTGACATTGAAAACCTTGAGGGCCCA
  GAGATCTTTTTTGAGGATGAGCTGGTGTGTATCCTAAACATGGAAGGAAGGAAAGCTTTGACCTGGAAAT
  ACTACGCAAAGAAAATTCTCTACTACCTGCGGCAGCAGAAAATCCTCAATAACCTCAAGGCGTTCCTGCA
  GCAGCCCGACGATTACGAGTCCTATCTCGAAGGTGCCGTTTACATCGACCAGTACTGCAACCCACTCTCC
  GACATCAGCTTCAGAGACATCCAGGCCCAGATCCACAGCATTGTGGAGCTTGTGTGCAAAACCCTCCGTG
  GCATCAACAGCCGCCACCCCAGCCTGACCTTCAGGGCAGGTGAGTCCTCTATGATAATGGAGATAGAACT
  TCAGAGCCAAGTGCTGGACGCTATCAACTATGTCCTGTATGACCAGCTGAAGTTCAAAGGGAACCGCATG
  GACTACTATAATGCCTTGAACCTCTACATGCACCAGGTGTTGACCCGCAGAACAGGAATCCCCATCAGCA
  TGTCTCTGCTCTACCTGACCGTCGCCCGGCAGCTGGGGGTTCCCCTGGAGCCTGTCAACTTCCCAAGCCA
  CTTCCTGCTGCGGTGGTGCCAAGGTGCAGAAGGGGCAACCCTGGACATCTTTGACTACATCTACATAGAT
  GCTTTTGGGAAGGGCAAGCAGCTGACAGTGAAAGAGTGTGAGTACCTGATTGGCCAGCACGTGACGGCAG
  CACTCTACGGCGTGGTGAATGTGAAGAAGGTGCTACAGCGGATGGTGGGCAACTTGCTGAGCCTGGGCAA
  GAGGGAAGGCATTGACCAGTCCTACCAGCTCCTCAGAGACTCCTTGGACCTGTACCTGGCGATGTACCCG
  GACCAGGTGCAGCTCCTGCTCCTCCAAGCCAGACTCTACTTCCACCTGGGCATCTGGCCAGAGAAGTCTT
  TCTGTCTTGTCTTGAAGGTGCTTGACATCCTCCAGCACATCCAGACCCTGGACCCCGGGCAGCACGGGGC
  GGTGGGCTATCTCGTGCAGCATACCTTAGAGCACATTGAGCGTAAGAAGGAGGAGGTGGGCGTGGAGGTG
  AAGCTGCGCTCCGAGGAGAAGCACAGGGATGTCTGCTACTCCATCGGGCTTGTGATGAAGCATAAGAGGT
  ACGGCTACAACTGTGTGATCTACGGCTGGGACCCTACGTGCATGATGGGGCATGAGTGGATCCGAAACAT
  GAATGTGCACAGCCTGCCTCATGGCCACCATCAGCCCTTCTACAACGTCCTGGTGGAGGATGGCTCCTGC
  AGATATGCAGCCCAAGAAAACCTGGAATACAACGTGGAGCCTCAGGAAATCTCGCACCCAGATGTGGGGC
  GCTACTTCTCGGAGTTCACAGGCACACACTACATCCCAAATGCAGAGCTGGAGATCCGGTACCCCGAGGA
  CCTGGAGTTTGTGTACGAGACAGTGCAGAATATTTACAGTGCAAAGGAGGACACAGCCGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209562 representing NM_145564
  Red=Cloning site Green=Tags(s)
  
  MASVAGDSAMEVVPALAEEAAAEATGPSCLVQLPGEVLEYILCSGSLTALDIGRVSSTCRRLREVCQSSG
  QVWKEQFRVRWPSLMKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGP
  EIFFEDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQYCNPLS
  DISFRDIQAQIHSIVELVCKTLRGINSRHPSLTFRAGESSMIMEIELQSQVLDAINYVLYDQLKFKGNRM
  DYYNALNLYMHQVLTRRTGIPISMSLLYLTVARQLGVPLEPVNFPSHFLLRWCQGAEGATLDIFDYIYID
  AFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKVLQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYP
  DQVQLLLLQARLYFHLGIWPEKSFCLVLKVLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEV
  KLRSEEKHRDVCYSIGLVMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSC
  RYAAQENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKEDTAE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145564
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145564.4
• RefSeq Size: 3909 bp
• RefSeq ORF: 1884 bp
• Locus ID: 231670
• UniProt ID: Q8VDH1
• Cytogenetics: 5 F
• Gene Summary: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.[UniProtKB/Swiss-Prot Function]