Unc93b1 (NM_019449) Mouse Tagged ORF Clone

CAT#: MG209291

Unc93b1 (tGFP-tagged) - Mouse unc-93 homolog B1 (C. elegans) (Unc93b1)

Product Images

Circular map for MG209291

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Unc93b1
• Synonyms: Unc-93B1; Unc93b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209291 representing NM_019449
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGAGCCTCCGCTCTACCCTGTGGCCGGGGCCGCGGGTCCTCAAGGGGATGAAGACCGGCACG
  GAGTTCCTGATGGGCCAGAGGCTCCCTTGGACGAACTCGTGGGTGCGTACCCCAACTACAATGAGGAGGA
  GGAAGAGCGCCGCTACTACCGCCGCAAGCGCCTCGGAGTGGTCAAGAACGTGCTGGCGGCCAGCACGGGT
  GTCACCCTTACTTACGGCGTCTACCTGGGTCTCCTGCAGATGCAACTGATCCTGCACTATGATGAGACCT
  ACAGAGAGGTGAAGTATGGCAACATGGGGCTGCCGGACATCGATAGCAAGATGCTGATGGGTATCAACGT
  GACGCCTATCGCTGCCCTGCTCTACACACCTGTGCTCATCAGGTTTTTTGGTACCAAGTGGATGATGTTC
  TTGGCTGTGGGCATCTATGCCCTCTTTGTCTCTACCAACTACTGGGAACGCTACTACACGCTGGTGCCCT
  CTGCTGTGGCTCTGGGCATGGCCATTGTGCCTCTGTGGGCCTCCATGGGCAACTATATCACCAGGATGTC
  CCAGAAGTACTATGAATACTCCCACTACAAGGAGCAAGATGAGCAGGGACCTCAGCAGCGCCCACCACGA
  GGCTCCCACGCACCCTATCTCCTGGTTTTCCAGGCCATCTTCTATAGCTTCTTCCACTTGAGCTTCGCGT
  GTGCCCAGCTGCCCATGATTTACTTCCTCAACAACTACCTGTATGACCTGAACCACACACTGATCAACGT
  GCAGAGCTGCGGTACTAAGAGCCAAGGCATTCTGAATGGCTTCAACAAGACGGTCCTTCGGACGCTGCCG
  CGCAGCAAAAACCTTATTGTTGTAGAGAGCGTGCTCATGGCGGTGGCCTTCTTGGCCATGCTGATGGTGC
  TGGGCCTGTGTGGAGCCGCTTACCGGCCCACGGAAGAGATCGACCTGCGCAGTGTGGGCTGGGGCAACAT
  CTTCCAGCTGCCCTTCAAACACGTCCGTGACTTTCGCTTACGCCATCTGGTGCCCTTCTTTATCTACAGT
  GGCTTTGAGGTGCTCTTTGCCTGCACTGGTTTTGCCCTGGGCTACGGCGTGTGCTCCATGGGGCTGGAGC
  GACTGGCATACCTGCTCATAGCTTACAGCCTGGGTGCCTCAGCCTCCTCGGTTCTGGGGCTGCTGGGACT
  GTGGCTGCCTCGCTCTGTCCCGCTCGTGGCTGGGGCAGGACTGCACCTACTGCTCACCCTTAGCCTCTTT
  TTCTGGGCTCCTGCTCCTCGGGTCCTCCAGCACAGTTGGATCTTTTACTTCGTGGCTGCCCTCTGGGGTG
  TGGGCAGCGCCCTCAACAAGACCGGACTTAGCACACTCCTGGGCATCCTATATGAAGACAAAGAGAGGCA
  GGACTTCATCTTCACCATCTATCACTGGTGGCAGGCCGTGGCCATCTTTGTTGTGTACCTGGGCTCCAGC
  TTGCCCATGAAGGCCAAGCTGGCAGTGTTGCTGGTGACCCTGGTAGCAGCAGCAGCCTCATACCTGTGGA
  TGGAACAGAAGTTGCAGCAAGGATTGGTCCCGCGGCAGCCGCGCATTCCGAAGCCACAGCACAAAGTCCG
  CGGCTACCGCTACCTGGAGGAGGACAACTCGGATGAGAGTGACATGGAGGGCGAGCAGGGTCAGGGGGAC
  TGCGCAGAGGACGAAGCACCACAGGCAGGGCCCCTGGGTACAGAGCCAGCTGGCCCCTGCCGCAAGCCCT
  GTCCCTATGAACAGGCTCTGGGTGGCGATGGGCCTGAGGAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209291 representing NM_019449
  Red=Cloning site Green=Tags(s)
  
  MEVEPPLYPVAGAAGPQGDEDRHGVPDGPEAPLDELVGAYPNYNEEEEERRYYRRKRLGVVKNVLAASTG
  VTLTYGVYLGLLQMQLILHYDETYREVKYGNMGLPDIDSKMLMGINVTPIAALLYTPVLIRFFGTKWMMF
  LAVGIYALFVSTNYWERYYTLVPSAVALGMAIVPLWASMGNYITRMSQKYYEYSHYKEQDEQGPQQRPPR
  GSHAPYLLVFQAIFYSFFHLSFACAQLPMIYFLNNYLYDLNHTLINVQSCGTKSQGILNGFNKTVLRTLP
  RSKNLIVVESVLMAVAFLAMLMVLGLCGAAYRPTEEIDLRSVGWGNIFQLPFKHVRDFRLRHLVPFFIYS
  GFEVLFACTGFALGYGVCSMGLERLAYLLIAYSLGASASSVLGLLGLWLPRSVPLVAGAGLHLLLTLSLF
  FWAPAPRVLQHSWIFYFVAALWGVGSALNKTGLSTLLGILYEDKERQDFIFTIYHWWQAVAIFVVYLGSS
  LPMKAKLAVLLVTLVAAAASYLWMEQKLQQGLVPRQPRIPKPQHKVRGYRYLEEDNSDESDMEGEQGQGD
  CAEDEAPQAGPLGTEPAGPCRKPCPYEQALGGDGPEEQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019449
• ORF Size: 1794 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019449.1, NP_062322.1
• RefSeq Size: 2277 bp
• RefSeq ORF: 1860 bp
• Locus ID: 54445
• UniProt ID: Q8VCW4
• Cytogenetics: 19 A
• Gene Summary: Plays an important role in innate and adaptive immunity by regulating nucleotide-sensing Toll-like receptor (TLR) signaling. Required for the transport of a subset of TLRs (including TLR3, TLR7 and TLR9) from the endoplasmic reticulum to endolysosomes where they can engage pathogen nucleotides and activate signaling cascades. May play a role in autoreactive B-cells removal.[UniProtKB/Swiss-Prot Function]