Tkfc (NM_145496) Mouse Tagged ORF Clone
CAT#: MG209059
- TrueORF®
Dak (tGFP-tagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_145496" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Tkfc |
Synonyms | BC021917; Dak |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG209059 representing NM_145496
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCAAGAAGATGGTGAATTCTGTGGAAGGGTGTGCTGATGATGCCCTTGCTGGGTTAGTAGCCT CTAACCCTGACTTGCAGCTCCTGCAAGGGCACCGTGTGGCCCTACGTTCTGACCTGGACACCCTCAAGGG CCGGGTAGCACTTCTGTCAGGTGGAGGCTCAGGCCATGAGCCTGCCCATGCCGGTTTTATCGGGAAAGGG ATGCTAACAGGTGTCATCGCAGGATCTGTGTTTGCCTCTCCTCCTGTGGGCAGCATCCTGGCAGCCATTA GAGCTGTGGCCCAGGCAGGCACAGTGGGCACCCTCCTCATTGTGAAGAACTACACTGGGGATCGGCTCAA CTTTGGACTTGCCATGGAGCAGGCCAAGGCCGAGGGCATCTCTGTGGAGATGGTGATAGTTGAGGATGAC AGTGCCTTCACCGTCCTCAAGAAGGCAGGCCGGCGTGGCCTGTGTGGCACAGTACTTATCCACAAGGTGG CAGGTGCTCTGGCTGAGGAAGGAATGGGGCTGGAGGAGATCACAAAGAGGGTGAGCGTGATCGCCAAGAC CATGGGTACCCTAGGGGTGAGCTTGTCATCCTGCAGTGTCCCTGGCGCCACACACACCTTTGAGCTTGCA GCTGATGAAATAGAGCTAGGCCTGGGGATCCATGGGGAAGCTGGTGTTCGTCGGATAAAGATAGCACCTG TTGATCAGATTGTCACTCTTATGCTTGACCACATGACAAACACCTCCAATATATTTCATGTGCCTGTACG GTCAGGCTCTTCAGTGGTGCTGATAGTCAACAATCTGGGTGGCCTGTCTTTCCTGGAACTGGGCATCATA GCTGATGCTGCCATACGCTTGCTGGAGGGCCGTGGAGTGAAGGTCGCCCGTGCCCTGGTGGGCACCTTCA TGTCAGCGCTGGAGATGCCTGGTGTGTCTCTTACTTTGATGCTTGTGGATGAACCCGTGCTGAAGCTAAT AGATGCTGAAACTACTGCAAAAGCCTGGCCTCACATGGCCAAGGTCTCTGTGACTGGGAGGAAGCGGATT CGGGCAGCTCCCACAGAGCCTCCAGAAGCCCCTGAAGCCACTGCAGCAGGAGGTGTAACATCAAAGCAGA TGGCACTTGTGCTGGATCGGATATGCACCACCCTCATCGGACTGGAGGAGCACCTGAATGCCTTGGACAG GGCTGCTGGTGATGGGGATTGTGGTTCTACCCATAGCCGTGCTGCCAAAGCCATTCAGGGCTGGCTAAAG GAAGGCCCATCTCTAACCAGCCCTGCCCAGGTACTCTCCAGATTGTCTGTCCTGCTGCTGGAGAGGATGG GAGGCTCATCTGGGGCGCTCTATGGCCTGTTCCTGACTGCAGCTGCCCAGCCTCTCAAGGCCAAGACTGA CCTCCCAACCTGGTCTGCTGCCATGGATGCCGGCTTAGAGTCCATGCAGAAGTATGGAAAGGCTGCACCA GGAGACAGGACAATGCTGGATTCTCTGTGGGCAGCAGCACAGGAGTTCCAAGCCTGGAAGAGCCCAGGGG CCAGTCTCCTCCCAGTCCTGACTAAAGCAGTCAAGAGTGCTGAAGCTGCAGCCGAGGCCACCAAGAATAT GGAAGCTGGTGCTGGGAGAGCTAGTTATATCAGCTCTGCACAACTAGATCAGCCAGACCCTGGAGCAGTC GCAGCTGCTGCCATCTTCCGTGCCATCCTGGAGGTCTTGCAGACGCAGGGAGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209059 representing NM_145496
Red=Cloning site Green=Tags(s) MSSKKMVNSVEGCADDALAGLVASNPDLQLLQGHRVALRSDLDTLKGRVALLSGGGSGHEPAHAGFIGKG MLTGVIAGSVFASPPVGSILAAIRAVAQAGTVGTLLIVKNYTGDRLNFGLAMEQAKAEGISVEMVIVEDD SAFTVLKKAGRRGLCGTVLIHKVAGALAEEGMGLEEITKRVSVIAKTMGTLGVSLSSCSVPGATHTFELA ADEIELGLGIHGEAGVRRIKIAPVDQIVTLMLDHMTNTSNIFHVPVRSGSSVVLIVNNLGGLSFLELGII ADAAIRLLEGRGVKVARALVGTFMSALEMPGVSLTLMLVDEPVLKLIDAETTAKAWPHMAKVSVTGRKRI RAAPTEPPEAPEATAAGGVTSKQMALVLDRICTTLIGLEEHLNALDRAAGDGDCGSTHSRAAKAIQGWLK EGPSLTSPAQVLSRLSVLLLERMGGSSGALYGLFLTAAAQPLKAKTDLPTWSAAMDAGLESMQKYGKAAP GDRTMLDSLWAAAQEFQAWKSPGASLLPVLTKAVKSAEAAAEATKNMEAGAGRASYISSAQLDQPDPGAV AAAAIFRAILEVLQTQGA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145496 |
ORF Size | 1734 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145496.1, NP_663471.1 |
RefSeq Size | 2350 bp |
RefSeq ORF | 1737 bp |
Locus ID | 225913 |
UniProt ID | Q8VC30 |
Cytogenetics | 19 A |
Gene Summary | Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219223 | Dak (untagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak), (10ug) |
USD 593.00 |
|
MR209059 | Dak (Myc-DDK-tagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak) |
USD 538.00 |
|
MR209059L3 | Lenti ORF clone of Dak (Myc-DDK-tagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak) |
USD 838.00 |
|
MR209059L4 | Lenti ORF clone of Dak (mGFP-tagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak) |
USD 838.00 |
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