Sema5a (BC065137) Mouse Tagged ORF Clone
CAT#: MG208910
- TrueORF®
Sema5a (tGFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"BC065137" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Sema5a |
Synonyms | 5930434A13, semF |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208910 representing BC065137
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGGAGCCTGCATCCTTGCATGGCTGTTCTCAAGCCTGGGAGTGTGGAGACTTGCTAGGCCCGAGA CCCAGGACCCTGCCAAGTGCCAGAGAGCTGAGCACCCTGTCGTCTCTTACAAAGAAATTGGCCCCTGGTT ACGGGAGTTCAGAGCCGAGAATGCTGTGGATTTCTCGAGGTTAACATTTGACCCAGGACAGAAAGAACTT GTCGTAGGAGCGAGAAACTATCTCTTCAGATTAGAGCTTGAGGATCTGTCTCTCATCCAGGCTGTGGAGT GGGAGTGTGACGAAGCCACCAAGAAGGCCTGTTACAGCAAGGGCAAATCGAAGGAGGAATGTCAGAACTA CATACGAGTGCTCTTAGTTGGTGGGGACCGGCTATTCACCTGTGGGACCAATGCCTTCACACCTGTCTGT ACCATCCGCTCGTTAAGTAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCCCGCTGTCCCTACA GTCCCCAGCACAATTCTACCGCCCTACTCACTGCCAGTGGTGAGCTCTATGCTGCAACAGCCATGGATTT CCCAGGGCGAGATCCAGCCATTTACCGAAGTCTGGGCACTTTGCCTCCTCTTCGAACTGCACAGTACAAC TCCAAATGGCTCAATGAACCAAACTTTGTGTCTTCCTACGACATCGGAAATTTCACCTACTTCTTCTTCC GAGAAAACGCCGTGGAACATGACTGTGGGAAGACGGTGTTCTCCAGGGCTGCCCGGGTCTGTAAAAATGA CATTGGAGGGCGATTTCTTCTGGAGGACACCTGGACTACCTTCATGAAGGCTCGCCTCAACTGCTCCCGG CCTGGTGAGGTGCCCTTCTACTACAATGAGCTGCAGGGTACTTTCTTCCTGCCGGAGCTGGATCTGATCT ATGGTATCTTCACCACCAATGTAAACAGCATCGCTGCCTCTGCTGTCTGTGTCTTCAACCTGAGCGCCAT CTCACAGGCCTTCAATGGACCCTTCAAGTACCAAGAAAACTCTCGCTCAGCCTGGCTGCCTTATCCCAAC CCCAACCCCAATTTTCAGTGCGGCACCATGGACCAGGGCCTGTACGTAAACCTGACGGAAAGAAACCTAC AGGATGCTCAGAAGTTCATTCTGATGCATGAAGTGGTGCAGCCAGTGACCACAGTGCCCTCTTTCATGGA GGACAACAGCCGCTTCTCCCACTTAGCTGTTGATGTTGTGCAAGGCAGGGAGACTCTGGTCCACATCATT TATCTGGCCACAGATTATGGCACCATTAAGAAAGTACGAGCTCCCCTGAGTCAGAGCTCAGGCAGCTGTT TGCTGGAAGAGATTGAGCTTTTCCCAGAGAGGAGGAGTGAGCCCATCAGGAGCCTGCAGATCCTCCACAG CCAGAGTGTCCTATTTGTGGGACTACAGGAGCATGTGGCCAAGATCCCCCTGAAGAGGTGCCACTTCCAT CAAACACGCAGTGCCTGCATTGGCGCTCAGGACCCTTACTGTGGCTGGGATGCGGTGATGAAGAAATGCA CCAGCCTGGAGGAGAGTCTGAGCATGACCCAGTGGGATCAGAGCATCCCCACCTGTCCGACCAGAAATCT CACTGTAGATGGGAGCTTTGGCCCATGGTCACCGTGGACACCCTGTACACACACTGATGGCATCTGGGCT GGTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208910 representing BC065137
Red=Cloning site Green=Tags(s) MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKEL VVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVC TIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYN SKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSR PGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPN PNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHII YLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFH QTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGIWA GV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC065137 |
ORF Size | 1688 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC065137, AAH65137 |
RefSeq Size | 4259 bp |
RefSeq ORF | 1688 bp |
Locus ID | 20356 |
Cytogenetics | 15 13.02 cM |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217745 | Sema5a (untagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic, (10ug) |
USD 524.00 |
|
MR208910 | Sema5a (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic |
USD 523.00 |
|
MR208910L3 | Lenti ORF clone of Sema5a (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic |
USD 823.00 |
|
MR208910L4 | Lenti ORF clone of Sema5a (mGFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic |
USD 823.00 |
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