Clec4f (NM_016751) Mouse Tagged ORF Clone

CAT#: MG208727

Clec4f (tGFP-tagged) - Mouse C-type lectin domain family 4, member f (Clec4f)

Product Images

Circular map for MG208727

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Clec4f
• Synonyms: Clecsf13; D18063; Kclr; KUCR_MOUSE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208727 representing NM_016751
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGGCCGAGCTGAACAGAGATATGGCTAGATATTGCACAGACAACCAGTGTGTCTCCCTACAGC
  CCCAAGGACTGGGGCCTAAGTCAGCTGCTCTCATGGCTCCCAGGACCCTGCGGCACGTCCAGGTCATTCT
  GGCATTAATGGTGGTGACTGTGATCTTTTCTCTCCTTGCTCTTTTTGTGGTGGCTTCACAGCCTTGGAGA
  CCTGAGTGGAATAAAGAGCCTCCCTCCCTCCTGCTTCGGGGAAGCAACAACTCTGGACACGACAATCATT
  CCCAATTTGTCAGGGAGACAGAAATGCAAGTGGCTATCCAGAGGCTTAGGGACTATGAGGAGAATTCTAG
  CAGCTGTCACAAGGAGGTACAGATACTGAAGTACCAAATGGACAATGTTAGTTCTCTGGTGCAGTTGCTT
  GGGAGCCATCTAGAGGATGTGAATGCTGACATCCTGCAGACAAAAGATGTGCTGAAGGAATCTGGAGCCT
  TGGCTTTGGAGACCCAGGCGCTGAGAAGCTCCTTGGAGGTGGCCAGTGCTGATATCCATAGTCTGAGAGG
  AGATTTGGAAAAGGCAAACGCTATGACTTCCCAGACCCGAGGTCTCTTAAAGAGCAGCACAGAAAACACC
  AGTGCTGAGCTCCACGTGCTGGGCAGAGGCCTGGAGGAAGCCCAGTCTGAGATCCAGGCGTTGAGGGGAA
  GTCTGCAGAGTGCAAATGACCTCAGCTCCCAGACCCAGGGCTTTCTCCAGCACAGTATGGACAACATTAG
  TGCTCAGATCCAGACCGTGAGAGATGGTATGGAAAGGGCTGGTGAAAAGATGAACTCGTTAAAGAAAGAG
  CTGGAAACACTCACTGCTCAGACTCAAAAGGCAAATGGCCACCTGGAGCAGACAGATGCCCAGATCCAGG
  GCTTAAAAGCTGAGCTGAAAAGCACCAGTTCCTTGAACTCCCGGATTGAGGTGGTCAATGGCCAAATGAA
  AGATGCCAGCAGAGAGTTACAGACCCTGAGAAGGGACCTGAGTGATGTCTCAGCTTTGAAGTCCAATGTC
  CAGATGCTACAGAGCAATCTTCAGAGGGCCAAGACAGAGATGCAGACTTTGAAGGCAGATCTGCAGGCGA
  CCAAAGCCCTCACTGCCAAGATTCAGGGGGAGCAGAATCGCCTGGGGGCCCTGCAGGAAGCCGTGGCTGC
  ACAGAAGCAGGAGCAGAAGACTCAGAATCAGGTTCTCCAGCTGATCGCGCAGAACTGGAAGTACTTCAAT
  GGAAACTTTTATTACTTTTCTCGTGACAAGAAGCCGTGGCGGGAGGCTGAGAAGTTCTGTACGTCCCAGG
  GAGCGCACCTGGCTTCGGTGACCTCTCAGGAGGAACAGGCATTCCTGGTACAGACTACAAGTTCTGGGGA
  CCATTGGATTGGGCTCACTGACCAGGGCACAGAGGGCATCTGGCGCTGGGTAGATGGGACACCATTCAAC
  AATGCCCAGAGCAAAGGGTTTTGGGGGAAAAATCAGCCTGACAACTGGAGACATAGGAACGGAGAGCGAG
  AAGACTGTGTTCACGTCCGGCAGCAGTGGAATGACATGGCCTGTGGATCCTCCTACCCCTGGGTGTGCAA
  GAAGTCCACAGGGTGGTCTGCGGCCAGAGTAGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208727 representing NM_016751
  Red=Cloning site Green=Tags(s)
  
  MKEAELNRDMARYCTDNQCVSLQPQGLGPKSAALMAPRTLRHVQVILALMVVTVIFSLLALFVVASQPWR
  PEWNKEPPSLLLRGSNNSGHDNHSQFVRETEMQVAIQRLRDYEENSSSCHKEVQILKYQMDNVSSLVQLL
  GSHLEDVNADILQTKDVLKESGALALETQALRSSLEVASADIHSLRGDLEKANAMTSQTRGLLKSSTENT
  SAELHVLGRGLEEAQSEIQALRGSLQSANDLSSQTQGFLQHSMDNISAQIQTVRDGMERAGEKMNSLKKE
  LETLTAQTQKANGHLEQTDAQIQGLKAELKSTSSLNSRIEVVNGQMKDASRELQTLRRDLSDVSALKSNV
  QMLQSNLQRAKTEMQTLKADLQATKALTAKIQGEQNRLGALQEAVAAQKQEQKTQNQVLQLIAQNWKYFN
  GNFYYFSRDKKPWREAEKFCTSQGAHLASVTSQEEQAFLVQTTSSGDHWIGLTDQGTEGIWRWVDGTPFN
  NAQSKGFWGKNQPDNWRHRNGEREDCVHVRQQWNDMACGSSYPWVCKKSTGWSAARVG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016751
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016751.2, NP_058031.2
• RefSeq Size: 2402 bp
• RefSeq ORF: 1647 bp
• Locus ID: 51811
• UniProt ID: P70194
• Cytogenetics: 6 C3
• Gene Summary: Receptor with an affinity for galactose and fucose. Could be involved in endocytosis.[UniProtKB/Swiss-Prot Function]