Enah (BC062927) Mouse Tagged ORF Clone

CAT#: MG208646

Enah (tGFP-tagged) - Mouse enabled homolog (Drosophila) (cDNA clone MGC:73459 IMAGE:6851217)

Product Images

Circular map for MG208646

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Enah
• Synonyms: Mena, WBP8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208646 representing BC062927
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAACAGAGTATCTGTCAGGCAAGAGCTGCTGTGATGGTCTATGATGATGCCAATAAGAAGTGGG
  TGCCAGCTGGTGGCTCAACTGGGTTCAGCAGAGTACATATATATCACCATACAGGCAACAACACATTCAG
  AGTTGTGGGCAGAAAGATTCAAGACCATCAGGTTGTGATAAACTGTGCCATTCCTAAAGGGCTGAAGTAC
  AATCAAGCTACACAGACTTTCCACCAATGGAGGGATGCTAGACAGGTGTATGGTCTCAACTTTGGCAGCA
  AAGAGGATGCCAATGTCTTCGCAAGTGCCATGATGCATGCCTTAGAAGTGTTAAATTCACAGGAAGCAGG
  GCCAACATTGCCTAGACAAAATTCACAGCTACCTGCTCAAGTTCAAAATGGCCCATCCCAAGAAGAGCTG
  GAAATCCAGAGAAGGCAACTGCAAGAACAGCAGCGACAGAAGGAACTGGAGAGGGAAAGAATGGAGAGGG
  AAAGGTTGGAGAGAGAACGACTAGAACGAGAGAGGCTAGAGAGGGAGCGCCTGGAACAAGAGCAGCTGGA
  GCGGCAGCGGCAGGAAAGGGAGCACGTGGAGCGGCTGGAGAGGGAGAGGCTGGAGCGCCTGGAGCGAGAG
  AGGCAGGAGCGGGAGCGAGAGCGCCTGGAGCAGCTGGAGCGGGAGCAAGTGGAGTGGGAGCGAGAGCGCA
  GAATGTCCAATGCTGCTGCCCCTGCCTCTGCGGAGACCCCTCTAAATCCTGAGCTGGGAGACTCCTCTGC
  TTCCGAGCCAGGCTTGCAGGCAGCCTCTCAGCCGGCCGAGTCGCCAACCCCACAGGGCCTTGTCTTGGGA
  CCACCTGCACCTCCGCCACCACCCCCTCTCCCATCAGGCCCTGCCTACGCCTCAGCACTTCCTCCTCCCC
  CAGGACCCCCTCCACCACCTCCACTGCCATCCACTGGTCCTCCTCCTCCACCCCCTCCACCACCCCCTCT
  TCCTAATCAAGCTCCTCCCCCTCCTCCCCCACCTCCTGCCCCTCCCCTCCCCGCATCTGGAATTTTCTCT
  GGATCCACGTCAGAAGACAATCGCCCTTTAACTGGACTTGCAGCTGCAATTGCGGGAGCAAAACTTAGGA
  AAGTGTCCCGGGTGGAGGATGGCTCTTTCCCAGGTGGAGGGAATACTGGGAGTGTGAGCTTGGCCTCATC
  CAAAGCAGACGCTGGGCGTGGGAATGGACCTCTTCCTCTAGGGGGTAGTGGCTTAATGGAAGAAATGAGT
  GCCCTGCTGGCCAGGAGGAGAAGAATTGCTGAGAAGGGATCAACAATAGAAACAGAACAAAAGGAAGACA
  GAAATGAAGATGCAGAGCCTATAACTGCTAAGGCCCCATCAACAAGTACACCTGAACCAACCAGAAAACC
  TTGGGAAAGAACAAACACAATGAACGGCAGTAAGTCACCTGTCATCTCCAGACCCAAATCCACACCTTCA
  TCACAGCCAAGTGCCAATGGAGTCCAGACAGAAGGCCTTGACTATGACAGGCTGAAGCAGGACATTTTAG
  ATGAGATGAGAAAAGAGCTGGCAAAGCTGAAGGAGGAGCTTATTGACGCAATCAGGCAGGAGCTGAGCAA
  GTCGAACACTGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208646 representing BC062927
  Red=Cloning site Green=Tags(s)
  
  MSEQSICQARAAVMVYDDANKKWVPAGGSTGFSRVHIYHHTGNNTFRVVGRKIQDHQVVINCAIPKGLKY
  NQATQTFHQWRDARQVYGLNFGSKEDANVFASAMMHALEVLNSQEAGPTLPRQNSQLPAQVQNGPSQEEL
  EIQRRQLQEQQRQKELERERMERERLERERLERERLERERLEQEQLERQRQEREHVERLERERLERLERE
  RQERERERLEQLEREQVEWERERRMSNAAAPASAETPLNPELGDSSASEPGLQAASQPAESPTPQGLVLG
  PPAPPPPPPLPSGPAYASALPPPPGPPPPPPLPSTGPPPPPPPPPPLPNQAPPPPPPPPAPPLPASGIFS
  GSTSEDNRPLTGLAAAIAGAKLRKVSRVEDGSFPGGGNTGSVSLASSKADAGRGNGPLPLGGSGLMEEMS
  ALLARRRRIAEKGSTIETEQKEDRNEDAEPITAKAPSTSTPEPTRKPWERTNTMNGSKSPVISRPKSTPS
  SQPSANGVQTEGLDYDRLKQDILDEMRKELAKLKEELIDAIRQELSKSNTA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC062927
• ORF Size: 1625 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062927, AAH62927
• RefSeq Size: 3942 bp
• RefSeq ORF: 1625 bp
• Locus ID: 13800
• Cytogenetics: 1 84.93 cM
• Gene Summary: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation.[UniProtKB/Swiss-Prot Function]