Cnot2 (NM_001037847) Mouse Tagged ORF Clone

CAT#: MG208625

Cnot2 (tGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (Cnot2), transcript variant 2

Product Images

Circular map for MG208625

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cnot2
• Synonyms: 2600016M12Rik; 2810470K03Rik; AA537049; AA959607; AW557563; C79650
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208625 representing NM_001037847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGGACTGATGGACATACATTATCTGAGAAAAGAAACTACCAGGTGACAAACAGCATGTTTGGTG
  CTTCAAGAAAGAAGTTTGTAGAGGGGGTGGACAGCGACTACCATGATGAGAACATGTACTACAGCCAGTC
  TTCTATGTTCCCACATCGGTCAGAGAAAGATATGCTGGCATCGCCATCTACGTCAGGTCAGCTGTCTCAA
  TTTGGGGCAAGTTTATACGGGCAACAAAGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAATACCC
  CTCAGTTAAATCGCAGCTTATCACAAGGCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGGGTACC
  AACAATGTCACTTCACACGCCTCCATCTCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAATATGATG
  AACCACTCCCAGGTTGGTCAGGGCATTGGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCAGGGTTAG
  GTAGCCCCAACAGAAGCTCGCCAAGCATAATATGTATGCCAAAGCAACAGCCTTCTCGACAGCCCTTTAC
  TGTGAACAGTATGTCTGGATTTGGAATGAACAGGAATCAGGCATTTGGAATGAATAACTCCTTATCAAGT
  AACATTTTTAATGGAACAGATGGCAGTGAAAATGTGACAGGATTGGACCTTTCAGATTTCCCAGCGTTAG
  CTGACCGGAATAGAAGGGAAGGGAGCGGCAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCC
  TTACGTTGGAATGGTAACAAAACCTGCAAATGAGCAATCCCAAGACTTCTCAATACACAACGAAGATTTT
  CCAGCATTACCTGGTTCCAGCTATAAAGATCCAACGTCAAGTAATGACGACAGCAAATCTAATTTGAGTA
  CATCGGGGAAGACGACTTCAAGTACAGATGGACCCAAATTCCCTGGAGATAAAAGTTCAACAACACAAAA
  CAATAATCAACAGAAAAAGGGGATCCAGGTGTTACCTGATGGTCGAGTTACTAACATTCCTCAAGGGATG
  GTGACGGACCAATTTGGAATGATTGGCCTGTTAACATTTATCAGGGCAGCAGAGACAGACCCAGGAATGG
  TACATCTTGCTTTAGGAAGTGACTTAACAACATTAGGCCTCAATCTGAACTCACCTGAAAATCTCTATCC
  CAAATTTGCATCACCCTGGGCTTCTTCACCTTGTCGGCCTCAAGACATAGACTTCCATGTTCCATCAGAA
  TATTTAACAAACATTCACATTAGGGATAAGCTGGCTGCAATCAAACTTGGCCGATATGGAGAAGACCTCC
  TCTTCTATCTGTATTACATGAACGGCGGGGATGTATTACAACTCTTAGCTGCGGTAGAACTTTTTAACCG
  TGATTGGAGATACCACAAGGAGGAGCGGGTATGGATTACCAGGGCACCAGGCATGGAGCCAACAATGAAA
  ACCAACACGTATGAGCGGGGGACCTACTATTTCTTTGACTGTCTCAACTGGAGGAAAGTAGCTAAGGAGT
  TCCATCTGGAATATGACAAATTAGAAGAGCGGCCTCACCTGCCATCCACCTTCAACTACAACCCTGCTCA
  GCAAGCCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208625 representing NM_001037847
  Red=Cloning site Green=Tags(s)
  
  MVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQ
  FGASLYGQQSALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMM
  NHSQVGQGIGIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSS
  NIFNGTDGSENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDF
  PALPGSSYKDPTSSNDDSKSNLSTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGM
  VTDQFGMIGLLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSE
  YLTNIHIRDKLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMK
  TNTYERGTYYFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037847
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037847.1, NP_001032936.1
• RefSeq Size: 2746 bp
• RefSeq ORF: 1653 bp
• Locus ID: 72068
• UniProt ID: Q8C5L3
• Cytogenetics: 10 D2
• Gene Summary: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.[UniProtKB/Swiss-Prot Function]