Atp6v1b2 (NM_007509) Mouse Tagged ORF Clone

CAT#: MG208205

Atp6v1b2 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B2 (Atp6v1b2)

Product Images

Circular map for MG208205

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp6v1b2
• Synonyms: AI194269; AI790362; Atp6b2; HO57; R74844
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208205 representing NM_007509
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTGCGAGCGATGCGGGGAATCGTGAACGGGGCCGCACCCGAACTGCCCGTGCCCACCGGTGGGC
  CGATGGCCGGAGCTCGGGAGCAGGCGCTGGCGGTGAGCCGGAACTACCTATCCCAGCCTCGTCTCACCTA
  CAAGACTGTCTCTGGGGTGAATGGTCCACTAGTGATCCTAGATCATGTGAAGTTTCCCAGATACGCTGAG
  ATTGTCCACTTGACATTACCAGATGGCACAAAGAGAAGTGGGCAAGTCCTAGAAGTTAGTGGCTCCAAAG
  CAGTGGTTCAGGTATTTGAAGGGACATCTGGTATAGACGCCAAGAAAACATCCTGTGAGTTTACTGGAGA
  TATTCTCCGAACACCAGTGTCTGAGGATATGCTTGGTCGAGTATTCAACGGATCAGGAAAACCCATTGAC
  CGAGGCCCTGTGGTGCTGGCTGAAGACTTCCTTGACATCATGGGTCAGCCAATCAACCCTCAGTGTCGGA
  TCTATCCAGAAGAGATGATTCAGACGGGCATTTCCGCCATCGATGGCATGAACAGTATTGCTAGGGGACA
  GAAAATCCCCATCTTTTCTGCTGCTGGATTACCACACAACGAGATTGCAGCTCAGATCTGTCGCCAGGCT
  GGTTTGGTAAAGAAATCCAAAGATGTAGTGGACTATAGTGAAGAAAATTTTGCCATTGTGTTTGCTGCTA
  TGGGAGTAAACATGGAAACAGCCCGGTTCTTTAAATCTGACTTTGAAGAAAATGGCTCAATGGACAATGT
  CTGCCTTTTTTTGAATCTGGCTAATGACCCAACCATTGAGCGGATCATCACTCCTCGCCTGGCTCTGACC
  ACGGCCGAGTTTCTGGCATATCAGTGTGAGAAGCACGTGCTGGTGATCCTGACGGACATGAGCTCCTACG
  CTGAAGCGCTGCGAGAGGTTTCAGCTGCCAGGGAAGAGGTCCCTGGTCGGCGAGGCTTCCCAGGCTACAT
  GTATACCGACTTAGCCACAATCTATGAACGTGCCGGTCGAGTGGAAGGTAGAAACGGCTCTATTACCCAA
  ATCCCTATTCTCACCATGCCCAATGACGATATCACTCATCCCATCCCTGACTTGACTGGGTACATTACTG
  AGGGCCAGATCTATGTGGACAGACAGCTGCACAACAGACAGATTTACCCTCCTATTAATGTGCTGCCCTC
  ACTCTCTCGGTTAATGAAGTCAGCTATCGGAGAAGGAATGACCAGAAAGGATCACGCTGATGTGTCTAAC
  CAGTTGTATGCGTGCTATGCCATCGGTAAGGACGTGCAAGCCATGAAAGCCGTGGTGGGAGAGGAAGCCC
  TGACCTCAGATGATCTCCTTTACCTGGAATTTCTGCAGAAGTTTGAGAAGAACTTCATTACTCAGGGTCC
  CTATGAAAACCGAACTGTTTATGAGACTTTGGACATTGGCTGGCAGTTGCTTCGAATCTTCCCCAAAGAA
  ATGCTGAAGAGAATCCCTCAGAGCACCCTGAGCGAATTTTACCCTCGAGACTCTGCAAAACAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208205 representing NM_007509
  Red=Cloning site Green=Tags(s)
  
  MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAE
  IVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPID
  RGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQA
  GLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALT
  TAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQ
  IPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSN
  QLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFEKNFITQGPYENRTVYETLDIGWQLLRIFPKE
  MLKRIPQSTLSEFYPRDSAKH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007509
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007509.3
• RefSeq Size: 2738 bp
• RefSeq ORF: 1536 bp
• Locus ID: 11966
• UniProt ID: P62814
• Cytogenetics: 8 B3.3
• Gene Summary: Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.[UniProtKB/Swiss-Prot Function]