Pdhx (NM_175094) Mouse Tagged ORF Clone
CAT#: MG208036
- TrueORF®
Pdhx (tGFP-tagged) - Mouse pyruvate dehydrogenase complex, component X (Pdhx)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_175094" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Pdhx |
Synonyms | AI481367; E3bp; Pdx1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208036 representing NM_175094
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTCCTGGAGGCTTCACTGTAACCAGCCGCTGCTTCGGTACCTTCTGGGATTTAGCAGCCGTC GAAGCCTGGGACTGGCTCAGGGTGCTGCTGCTTGGCCTGTCGACCGTGGAGCAAGTTGGAGGTGGTTTCA CAGTACTCAGCTGCTGCAGGCGGATCCTATTAAGGTCCTAATGCCATCACTGTCTCCTACGATGGAGCAA GGGAACATTGTGAAATGGCTGAGAAAGGAAGGTGAAGCTGTGAGTGCTGGAGACTCGTTATGTGAAATTG AGACGGACAAGGCTGTGGTCACCTTAGATGCAAACGATGATGGCATCTTAGCCAAGATAGTGGTTGAGGA AGGTGCTAAAAATATACAGCTGGGTTCACTCATTGCTTTGATGGTTGAAGAAGGGGAAGATTGGAAACAG GTGGAAATTCCCAAGGATGTCAGTGCTCCACCTCCAGTCTCAAAACCACCAGCTCCTACTCAGCCCTCTC CACAGCCGCAGATTCCTTGTCCCGCCAGGAAGGAGCACAAAGGGACGGCACGGTTCCGCTTAAGTCCAGC TGCCCGCAACATTCTGGAAAAACATTCATTGGACGCCAGCCAGGGCACAGCCACAGGCCCTCGCGGGATC TTCACTAAAGAAGATGCACTCAAGTTAGTCGAGCTGAAACAGATGGGCAAGATTACTGAATCCAGACCAG CCTCAGCTCCTCCACCCTCTCTCTCAGCATCTGTGCCTCCTCAGGCTACAGCCGGGCCATCCTATCCTCG GCCAATGACCCCACCAGTATCCATTCCTGGGCAACCGAATGCAGCGGGTACATTCACCGAAATTCCTGCT AGCAATATTCGAAGAGTTATCGCTAAGAGGTTAACTGAATCAAAAAGTACTGTGCCTCATGCCTATGCTA CTGCTGACTGTGACCTTGGCGCAGTTCTAAAGGTTAGACGAGATCTGGTCAAAGATGACATTAAAGTCTC AGTAAATGACTTTATCATCAGAGCAGCAGCTGTTACTCTTAAACAAATGCCTGGGGTTAATGTCACCTGG GATGGAGAAGGCCCAAAGCAGCTGCCCTCTGTTGACATTTCAGTGGCTGTGGCAACAGATAAAGGTTTAA TTACACCAATCATAAAAGATGCAGCCGCTAAGGGCATTCAGGAGATCGCTGACTCTGTAAAGGTTCTGTC GAAGAAAGCACGGGATGGGAAATTGATGCCAGAGGAGTACCAAGGAGGATCTTTTAGTATTTCCAACTTG GGGATGTTTGGCATCGATGAGTTCGCTGCAGTGATTAACCCGCCTCAGGCCTGTATCTTGGCCGTTGGGA GATTCCGCCCCGTGCTGAAGCTGACGGAGGATGAAGAGGGGAACCCTCAACTGCAGCAGCACCAGCTTAT AACGGTCACAATGTCAAGTGACAGCCGAGTGGTTGATGATGAACTAGCTACCAGGTTTCTTGAAACTTTC AAAGCAAACCTAGAGAATCCCATGCGACTTGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208036 representing NM_175094
Red=Cloning site Green=Tags(s) MAASWRLHCNQPLLRYLLGFSSRRSLGLAQGAAAWPVDRGASWRWFHSTQLLQADPIKVLMPSLSPTMEQ GNIVKWLRKEGEAVSAGDSLCEIETDKAVVTLDANDDGILAKIVVEEGAKNIQLGSLIALMVEEGEDWKQ VEIPKDVSAPPPVSKPPAPTQPSPQPQIPCPARKEHKGTARFRLSPAARNILEKHSLDASQGTATGPRGI FTKEDALKLVELKQMGKITESRPASAPPPSLSASVPPQATAGPSYPRPMTPPVSIPGQPNAAGTFTEIPA SNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRRDLVKDDIKVSVNDFIIRAAAVTLKQMPGVNVTW DGEGPKQLPSVDISVAVATDKGLITPIIKDAAAKGIQEIADSVKVLSKKARDGKLMPEEYQGGSFSISNL GMFGIDEFAAVINPPQACILAVGRFRPVLKLTEDEEGNPQLQQHQLITVTMSSDSRVVDDELATRFLETF KANLENPMRLG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_175094 |
ORF Size | 1503 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175094.5, NP_780303.1 |
RefSeq Size | 2527 bp |
RefSeq ORF | 1506 bp |
Locus ID | 27402 |
UniProt ID | Q8BKZ9 |
Cytogenetics | 2 E2 |
Gene Summary | Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216992 | Pdhx (untagged) - Mouse pyruvate dehydrogenase complex, component X (Pdhx), nuclear gene encoding mitochondrial protein, (10ug) |
USD 514.00 |
|
MR208036 | Pdhx (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase complex, component X (Pdhx), nuclear gene encoding mitochondrial protein |
USD 466.00 |
|
MR208036L3 | Lenti ORF clone of Pdhx (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase complex, component X (Pdhx), nuclear gene encoding mitochondrial protein |
USD 766.00 |
|
MR208036L4 | Lenti ORF clone of Pdhx (mGFP-tagged) - Mouse pyruvate dehydrogenase complex, component X (Pdhx), nuclear gene encoding mitochondrial protein |
USD 766.00 |
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