Fars2 (NM_024274) Mouse Tagged ORF Clone
CAT#: MG207194
- TrueORF®
Fars2 (tGFP-tagged) - Mouse phenylalanine-tRNA synthetase 2 (mitochondrial) (Fars2), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_024274" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Fars2 |
Synonyms | Fars1; pheRS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207194 representing NM_024274
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTGCTTGGCTCTCGTCAGAGCTGCCTATGAGCACATCTACCTGGTGAGGAAGGTCAGTCATGCCT GCAGATGCCATCAACATCGGGCTTGGAGCTCAAAGCCAGCTGCATCACAGTCTGCTGTCCAGGGTGCTCC AGGCAGTGTGCTGGAGATACTTGGTAAATCCTACCCTCAGGATGACCACACTAACCTCACCCAGAAGGTC CTTTCCAAGGTGGGCAGGAACCTGCACAACCAGAAGTTCCACCCTCTGTGGCTGATTAAGGAGCGGGTGA AGGAGCACTTCTACCAGCAGTATATGGTGCGATCCAGGACACCTCTATTCTCCGTCTATGACCAGCTTCC TCCAGTGGTCACCACCTGGCAGAACTTTGATAGCCTGCTAATCCCAGCTGACCACCCCAGCAGGAAAAAG GGGGACAACTATTACTTGAATCGGGCACACATGCTGAGAGCACACACATCAGCGCATCAGTGGGACTTGC TGCATGCGGGACTTAATGCCTTCCTTGTGGTAGGTGACGTGTATCGTCGTGACCAGATTGATTGCCAGCA CTACCCAGTTTTCCACCAGCTGGAGGGTGTCCGGCTCTTCTCCAAGCATGAGTTATTTGCTGGCGTAAAG GATGGAGAAAGCTTGCAGCTCTTTGAAGAAGGTTCTCGCTCTGCTCATAAGCAGGAGACACACACCATGG AGGCTGTGAAGCTTGTGGAGTTCGACCTTAAACAAGTACTGACTAGGCTTGTGACACATCTTTTTGGAGA TGGGCTGGAGGTTCGGTGGGTGGATTGCTACTTCCCCTTCACCCACCCTTCCTTCGAGATGGAGATCAAC TTCCGTGGAGAATGGCTTGAAGTCCTTGGCTGCGGTGTGATGGAGCAGCAGCTTGTCAATTCAGCTGGTG CTCAGGATCGAATCGGCTGGGCGTTTGGCCTGGGATTAGAAAGATTGGCGATGGTCCTCTACGACATTCC TGACATCCGCCTTTTCTGGAGTGAGGATGAGCGCTTCCTGAAGCAGTTCCTCCTGTCGGACATCAACCAG AGTGTCAAGTTTCAGCCTCTTAGCAAGTACCCCGCCGTGTTCAATGACATTTCATTCTGGCTGCCCTCTG AGAACTACACGGAGAATGATTTCTATGACATCGTCCGAACAGTCGGAGGAGACCTGGTAGAAAAGGTTGA TCTTATAGACAAGTTTGAACATCCAAAGACACACAGGACCAGCCACTGCTACCGCATTACCTACCGCCAC ATGGAACGGACACTGTCCCAGAGAGAAGTCGGCAATGTCCACCAGGCTGTGCAGGAGGCCGCCGTGCAGC TGCTGGGTGTGGAGGGCCGTTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207194 representing NM_024274
Red=Cloning site Green=Tags(s) MVCLALVRAAYEHIYLVRKVSHACRCHQHRAWSSKPAASQSAVQGAPGSVLEILGKSYPQDDHTNLTQKV LSKVGRNLHNQKFHPLWLIKERVKEHFYQQYMVRSRTPLFSVYDQLPPVVTTWQNFDSLLIPADHPSRKK GDNYYLNRAHMLRAHTSAHQWDLLHAGLNAFLVVGDVYRRDQIDCQHYPVFHQLEGVRLFSKHELFAGVK DGESLQLFEEGSRSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEIN FRGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMVLYDIPDIRLFWSEDERFLKQFLLSDINQ SVKFQPLSKYPAVFNDISFWLPSENYTENDFYDIVRTVGGDLVEKVDLIDKFEHPKTHRTSHCYRITYRH MERTLSQREVGNVHQAVQEAAVQLLGVEGRF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024274 |
ORF Size | 1893 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024274.3, NP_077236.1 |
RefSeq Size | 2140 bp |
RefSeq ORF | 1356 bp |
Locus ID | 69955 |
UniProt ID | Q99M01 |
Cytogenetics | 13 A3.3 |
Gene Summary | Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200336 | Fars2 (untagged) - Mouse phenylalanine-tRNA synthetase 2 (mitochondrial) (Fars2), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug) |
USD 457.00 |
|
MR207194 | Fars2 (Myc-DDK-tagged) - Mouse phenylalanine-tRNA synthetase 2 (mitochondrial) (Fars2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 457.00 |
|
MR207194L3 | Lenti ORF clone of Fars2 (Myc-DDK-tagged) - Mouse phenylalanine-tRNA synthetase 2 (mitochondrial) (Fars2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 757.00 |
|
MR207194L4 | Lenti ORF clone of Fars2 (mGFP-tagged) - Mouse phenylalanine-tRNA synthetase 2 (mitochondrial) (Fars2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 757.00 |
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