Phf6 (NM_027642) Mouse Tagged ORF Clone

CAT#: MG205605

Phf6 (tGFP-tagged) - Mouse PHD finger protein 6 (Phf6)

Product Images

Circular map for MG205605

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Phf6
• Synonyms: 2700007B13Rik; 4931428F02Rik; mKIAA1823
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205605 representing NM_027642
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAGCTCAATTGAACAGAAAAAAGGGTCTACGAGACAGCGTAAATGTGGCTTTTGTAAGTCAAATA
  GAGACAAGGAATGTGGACAGTTACTGATATCTGAAAACCAGAAGGTGGCAGCACACCACAAGTGCATGCT
  GTTTTCGTCTGCTTTGGTGTCCTCACATTCTGATAATGAGAGTCTTGGTGGATTTTCTATTGAAGATGTT
  CAAAAGGAAATTAAAAGAGGCACAAAGCTGATGTGTTCTCTTTGTCATTGTCCTGGAGCAACCATTGGCT
  GTGATGTGAAAACCTGCCACAGGACATACCACTACCACTGTGCATTGCATGATAAAGCTCAGATCCGAGA
  GAAACCTTCGCAAGGGATTTACATGGTTTATTGTCGAAAACACAAGAAAACTGCACATAACTCGGAAGCT
  GATTTAGAAGAAAGTTTTAATGAGCATGAACTGGAGCCCTCATCTCCAAAGACTAAGAAGAAAAGTCGCA
  AAGGAAGACCGAGAAAAACTAACCTTAAAGGCTTGCCAGAAGACAGCAGGTCCACATCCTCCCATGGAAC
  AGATGAAATGGAAAGTAGCTCCTACCGAGATAGGTCTCCACACAGGAGCAGTCCTAATGATACCAGGCCT
  AAATGTGGATTTTGCCATGTAGGGGAGGAAGAAAATGAAGCAAGAGGGAAATTGCATATATTTAATGCCA
  AGAAGGCAGCCGCACATTACAAGTGCATGTTGTTTTCTTCTGGCACTGTTCAGCTCACAACAACATCAAG
  AGCAGAATTTGGAGATTTCGATATTAAAACTGTACTTCAGGAGATTAAGCGAGGAAAAAGGATGAAATGT
  ACACTTTGCAGTCAGCCTGGTGCTACTATTGGATGCGAAATAAAAGCCTGTGTTAAAACCTACCATTACC
  ACTGTGGTGTACAAGACAAGGCAAAATACATTGAAAATATGTCACGAGGAATTTACAAACTCTATTGTAA
  AAACCATAGTGGAAATGATGAGCGAGATGAAGAAGATGAGGAGCGAGAGAGTAAAAGCCGTGGAAGAGTA
  GCGATTGATCAGCAGCTAACCCAGCAGCAGCTTAATGGAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205605 representing NM_027642
  Red=Cloning site Green=Tags(s)
  
  MSSSIEQKKGSTRQRKCGFCKSNRDKECGQLLISENQKVAAHHKCMLFSSALVSSHSDNESLGGFSIEDV
  QKEIKRGTKLMCSLCHCPGATIGCDVKTCHRTYHYHCALHDKAQIREKPSQGIYMVYCRKHKKTAHNSEA
  DLEESFNEHELEPSSPKTKKKSRKGRPRKTNLKGLPEDSRSTSSHGTDEMESSSYRDRSPHRSSPNDTRP
  KCGFCHVGEEENEARGKLHIFNAKKAAAHYKCMLFSSGTVQLTTTSRAEFGDFDIKTVLQEIKRGKRMKC
  TLCSQPGATIGCEIKACVKTYHYHCGVQDKAKYIENMSRGIYKLYCKNHSGNDERDEEDEERESKSRGRV
  AIDQQLTQQQLNGN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027642
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027642.2
• RefSeq Size: 4385 bp
• RefSeq ORF: 1095 bp
• Locus ID: 70998
• UniProt ID: Q9D4J7
• Cytogenetics: X A5
• Gene Summary: Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.[UniProtKB/Swiss-Prot Function]