Prkx (NM_016979) Mouse Tagged ORF Clone

CAT#: MG205408

Prkx (tGFP-tagged) - Mouse protein kinase, X-linked (Prkx)

Product Images

Circular map for MG205408

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prkx
• Synonyms: Pkare; PKX1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205408 representing NM_016979
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCCCGCTGGTGCCGCGGCGACCGTAAAAGATCCAGACCATGACCCCGTGAAGACCAAGGTCT
  CGGCGCCCGCCGCGGACCCCAAACCCAGGACATCGTCACAGAAGGCGGGGCACTCGTTACAAGATTGGGA
  CACCATAGCCACCGTGGGCACTGGGACCTTTGGCCGTGTAAACCTGGTGAAGGAGAAGACAGGCAGGCAA
  TACTGTGCCTTGAAGATTATGAGTATCCCAGATGTCATCCGCCTGAAACAGGAACAGCATGTGCAGAATG
  AGAAAGCAGTCCTGAAGGAAATAAACCATCCCTTCCTCATTAAACTGCTTTGGACAGGCCATGACAACCG
  CTTCCTATACATGTTGATGGAGTTCGTGCCTGGTGGTGAGCTCTTCACCTACCTGCGTAATCGTGGCCGA
  TTCTCCTCAGTTGCTTCAGTCTTCTACGCCACAGAGATTGTGTGTGCCATAGAATATCTACACTCAAAGG
  AAATCGTATACAGGGACCTGAAGCCTGAAAACATCCTTCTAGACAGGGAAGGACATATTAAACTGACAGA
  CTTCGGCTTCGCCAAGAAACTGGTGGATAGGACATGGACTCTCTGTGGGACACCAGAATATCTCGCCCCA
  GAAGTCATTCAGAGCAAAGGTCATGGAAGGGCTGTGGACTGGTGGGCACTGGGCATTCTTATATTCGAGA
  TGCTGTCTGGGTTTCCCCCATTTTTCGATGACAACCCGTTTGGGATTTACCAGAAAATCCTTGCATGCAA
  AATAGATTTCCCCAGACAATTGGATTTCACCTCTAAGGACCTCATCAAGAAGCTGCTGGTGGTGGACAGG
  ACCCGACGCCTAGGCAACATGAAGAATGGGGCAGAAGACATCAAGCGGCACCGGTGGTTCCGAGGTGTGG
  AGTGGGAATCTGTGCCACAAAGAAAACTGAAGCCACCCATTGTGCCCAAGTTATCGGGTGATGGTGACAT
  CTCCAACTTTGAGACTTATCCAGAGAGTGAATTGGACAAGACACCTTCTGTATCTGACAAAGACCTGGAA
  ACATTCAAAAATTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205408 representing NM_016979
  Red=Cloning site Green=Tags(s)
  
  MEPPAGAAATVKDPDHDPVKTKVSAPAADPKPRTSSQKAGHSLQDWDTIATVGTGTFGRVNLVKEKTGRQ
  YCALKIMSIPDVIRLKQEQHVQNEKAVLKEINHPFLIKLLWTGHDNRFLYMLMEFVPGGELFTYLRNRGR
  FSSVASVFYATEIVCAIEYLHSKEIVYRDLKPENILLDREGHIKLTDFGFAKKLVDRTWTLCGTPEYLAP
  EVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILACKIDFPRQLDFTSKDLIKKLLVVDR
  TRRLGNMKNGAEDIKRHRWFRGVEWESVPQRKLKPPIVPKLSGDGDISNFETYPESELDKTPSVSDKDLE
  TFKNF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016979
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016979.2
• RefSeq Size: 3217 bp
• RefSeq ORF: 1068 bp
• Locus ID: 19108
• UniProt ID: Q922R0
• Cytogenetics: X B
• Gene Summary: Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.[UniProtKB/Swiss-Prot Function]