Sfrp4 (NM_016687) Mouse Tagged ORF Clone
CAT#: MG205321
- TrueORF®
Sfrp4 (tGFP-tagged) - Mouse secreted frizzled-related sequence protein 4 (Sfrp4)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_016687" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Sfrp4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205321 representing NM_016687
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCGCTCCATCCTGGTGGCGTTATGCCTGTGGCTGCGCCTGGCGCTGGGAGTGCGCGGAGCGCCCT GCGAAGCTGTGCGCATCCCCATGTGCAGGCACATGCCCTGGAACATCACCCGGATGCCCAACCACCTGCA CCACAGCACTCAGGAGAACGCCATCCTGGCCATCGAACAGTATGAAGAGCTAGTGGACGTGAACTGCAGC TCTGTGCTGCGCTTCTTCCTTTGTGCCATGTACGCACCCATCTGTACCCTGGAGTTCCTGCACGATCCCA TCAAGCCCTGCAAGTCTGTGTGCCAGCGCGCACGTGACGACTGCGAGCCCCTCATGAAGATGTATAACCA CAGCTGGCCAGAGAGCCTGGCCTGCGATGAGCTGCCGGTCTATGACCGTGGAGTTTGTATCTCTCCAGAG GCAATAGTCACTGACCTTCCAGAAGATGTGAAGTGGATAGACATCACACCAGATATGATGGTGCAAGAAA GGTCCTTTGATGCTGACTGTAAACGTCTGAGCCCTGATCGGTGCAAGTGCAAAAAGGTGAAGCCGACCCT GGCAACATACCTGAGCAAAAACTACAGCTATGTTATTCATGCCAAAATAAAAGCCGTCCAGAGGAGTGGT TGCAATGAGGTCACAACTGTGGTAGATGTAAAAGAGATCTTCAAGTCTTTGTCACCTATCCCTCGAACAC AAGTCCCTCTCATCACCAATTCCTCCTGCCAGTGTCCACATATCCTGCCCCACCAAGATGTCCTAATCAT GTGTTATGAGTGGCGTTCAAGGATGATGCTTCTTGAAAATTGCTTAGTTGAAAAGTGGAGAGATCAACTC AGTAGAAGGTCCATACAGTGGGAAGAGAGGCTTCAGGAACAGCAGAGAACAATTCAGGACAAGAAGCAGA TAGCCAGCCGCACCAGTCGCACCAGTCGCAGTAACCCCCCAAAGTCAAAGGGAAGGCCACCTGCTCCCAA ACCTGCCAGTCCTAAGAAGAACATCAAAGCTAGAAGTGCCCCCAAAAAGTCAAACCTGAAGAAAAGCGCA AGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205321 representing NM_016687
Red=Cloning site Green=Tags(s) MLRSILVALCLWLRLALGVRGAPCEAVRIPMCRHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVNCS SVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHSWPESLACDELPVYDRGVCISPE AIVTDLPEDVKWIDITPDMMVQERSFDADCKRLSPDRCKCKKVKPTLATYLSKNYSYVIHAKIKAVQRSG CNEVTTVVDVKEIFKSLSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQL SRRSIQWEERLQEQQRTIQDKKQIASRTSRTSRSNPPKSKGRPPAPKPASPKKNIKARSAPKKSNLKKSA S TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016687 |
ORF Size | 1053 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016687.1, NP_057896.1 |
RefSeq Size | 1785 bp |
RefSeq ORF | 1056 bp |
Locus ID | 20379 |
UniProt ID | Q9Z1N6 |
Cytogenetics | 13 6.99 cM |
Gene Summary | Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (PubMed:27355534). SFRP4 plays a role in bone morphogenesis (PubMed:27355534). May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209381 | Sfrp4 (untagged) - Mouse secreted frizzled-related protein 4 (Sfrp4), (10ug) |
USD 503.00 |
|
MR205321 | Sfrp4 (Myc-DDK-tagged) - Mouse secreted frizzled-related protein 4 (Sfrp4) |
USD 457.00 |
|
MR205321L3 | Lenti ORF clone of Sfrp4 (Myc-DDK-tagged) - Mouse secreted frizzled-related protein 4 (Sfrp4) |
USD 757.00 |
|
MR205321L4 | Lenti ORF clone of Sfrp4 (mGFP-tagged) - Mouse secreted frizzled-related protein 4 (Sfrp4) |
USD 757.00 |
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