Fcgr4 (NM_144559) Mouse Tagged ORF Clone

CAT#: MG203178

Fcgr4 (tGFP-tagged) - Mouse Fc fragment of IgG, low affinity IIIa, receptor (Fcgr3a)

Product Images

Circular map for MG203178

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fcgr4
• Synonyms: 4833442P21Rik; CD16-2; FcgammaRIV; Fcgr3a; FcgRIV; Fcrl3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203178 representing NM_144559
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCAGCTACTACTACCAACAGCTCTGGTACTTACAGCTTTCTCTGGCATTCAAGCTGGTCTCCAAA
  AGGCTGTGGTGAACCTAGACCCCAAGTGGGTCAGGGTGCTTGAGGAAGACAGCGTGACCCTCAGATGCCA
  AGGCACTTTCTCCCCCGAGGACAATTCTATCAAGTGGTTCCATAACGAAAGCCTCATCCCACACCAGGAT
  GCCAACTATGTCATCCAAAGTGCCAGAGTTAAGGACAGTGGAATGTACAGGTGCCAGACAGCCCTCTCCA
  CGATCAGTGACCCAGTGCAACTAGAGGTCCATATGGGCTGGCTATTGCTTCAGACCACTAAGTGGCTGTT
  CCAGGAGGGGGACCCCATTCATCTGAGATGCCACAGTTGGCAAAACAGACCTGTACGGAAGGTCACCTAT
  TCACAGAACGGCAAAGGCAAGAAGTATTTCCATGAAAATTCTGAATTACTCATTCCAAAAGCTACACACA
  ATGACAGTGGCTCCTACTTCTGCAGAGGGCTCATTGGACACAACAACAAATCTTCAGCATCCTTTCGTAT
  AAGCCTAGGCGATCCAGGGTCTCCATCCATGTTTCCACCGTGGCATCAAATCACATTCTGCCTGCTGATA
  GGACTCTTGTTTGCAATAGACACAGTGCTGTATTTCTCTGTGCGGAGGGGTCTTCAAAGTCCTGTGGCTG
  ACTATGAGGAACCCAAGATTCAATGGAGCAAGGAACCTCAGGACAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203178 representing NM_144559
  Red=Cloning site Green=Tags(s)
  
  MWQLLLPTALVLTAFSGIQAGLQKAVVNLDPKWVRVLEEDSVTLRCQGTFSPEDNSIKWFHNESLIPHQD
  ANYVIQSARVKDSGMYRCQTALSTISDPVQLEVHMGWLLLQTTKWLFQEGDPIHLRCHSWQNRPVRKVTY
  SQNGKGKKYFHENSELLIPKATHNDSGSYFCRGLIGHNNKSSASFRISLGDPGSPSMFPPWHQITFCLLI
  GLLFAIDTVLYFSVRRGLQSPVADYEEPKIQWSKEPQDK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144559
• ORF Size: 747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144559.1, NP_653142.1
• RefSeq Size: 1304 bp
• RefSeq ORF: 750 bp
• Locus ID: 246256
• UniProt ID: A0A0B4J1G0
• Cytogenetics: 1 78.53 cM
• Gene Summary: Receptor for the Fc region of immunoglobulin gamma (PubMed:16039578). Also acts as a receptor for the Fc region of immunoglobulin epsilon (PubMed:17558411, PubMed:18949059). Binds with intermediate affinity to both IgG2a and IgG2b (PubMed:16039578, PubMed:17558411, PubMed:19795417). Can bind to IgG2a and IgG2b monomers (PubMed:18949059). Does not display binding to IgG1 or IgG3 (PubMed:16039578). Mediates neutrophil activation by IgG complexes redundantly with Fcgr3 (PubMed:18097064). Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a (PubMed:25824719). Binds with low affinity to both the a and b allotypes of IgE (PubMed:18949059). Has also been shown to bind to IgE allotype a only but not to allotype b (PubMed:17558411). Binds aggregated IgE but not the monomeric form and bound monomeric IgG is readily displaced by IgE complexes (PubMed:18949059). Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions (PubMed:17558411, PubMed:18949059).[UniProtKB/Swiss-Prot Function]