Ccdc115 (NM_027159) Mouse Tagged ORF Clone

CAT#: MG201615

Ccdc115 (tGFP-tagged) - Mouse coiled-coil domain containing 115 (Ccdc115)

Product Images

Circular map for MG201615

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ccdc115
• Synonyms: 2310061I09Rik; Ccp1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201615 representing NM_027159
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCCAGGCTCTGCGAGAGGAGTTGGACTCCAAGTGCCTACAGCTGCTCAGTGACCTGGAGGAAC
  TGGAGGCGAAGCGGGCGGCGCTGAACGCCAGGGTGGAGGAGGGTTGGCTCTCACTTGCCAAGGCTCGCTA
  TGCCATGGGAGCCAAGTCGGTAGGACCCCTGCAGTATGCCTCGCGTATGGAGCCTCAGGTCTGCGTGCGT
  GCCAGCGAGGCCCAGGATGGACCCCAGACCTTCAGGGTGATCAAAGCTGACGCCCAGACCCCCGAGGAAG
  TGGGGCCTAGCGAAGCATCTCTGCGCAGGCGCAAGGGCCCCACCAAGACCAAAGAGTTAGGATCTGCTGT
  AGTTCCTCAAGATCCCTTGAACTGGTTTGGAATTTTGGTTCCTCACAGTCTGCGGCAGGCCCAAGCCAGC
  TTCCGGGATGGCCTACAGCTGGCTGCAGATATAGCCAGTCTCCAGACTCGCATCAACTGGGGTCAGAGTC
  AGCTTCGAGGCCTCCAGAAAAAACTGAAGGAGTTGGACCCTGGGCCTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201615 representing NM_027159
  Red=Cloning site Green=Tags(s)
  
  MAVQALREELDSKCLQLLSDLEELEAKRAALNARVEEGWLSLAKARYAMGAKSVGPLQYASRMEPQVCVR
  ASEAQDGPQTFRVIKADAQTPEEVGPSEASLRRRKGPTKTKELGSAVVPQDPLNWFGILVPHSLRQAQAS
  FRDGLQLAADIASLQTRINWGQSQLRGLQKKLKELDPGPA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027159
• ORF Size: 540 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027159.2
• RefSeq Size: 1709 bp
• RefSeq ORF: 543 bp
• Locus ID: 69668
• UniProt ID: Q8VE99
• Cytogenetics: 1 B
• Gene Summary: Accessory component of the proton-transporting vacuolar (V)-ATPase protein pump involved in intracellular iron homeostasis. In aerobic conditions, required for intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. Necessary for endolysosomal acidification and lysosomal degradation (By similarity). May be involved in Golgi homeostasis (By similarity).[UniProtKB/Swiss-Prot Function]