Ccdc115 (NM_027159) Mouse Untagged Clone

CAT#: MC203144

Ccdc115 (untagged) - Mouse coiled-coil domain containing 115 (Ccdc115), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc115
• Synonyms: 2310061I09Rik; Ccp1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019430
  GCTTCACCGGTGCTTGAGCTGGTCAGGCAGCGGGCTAGCGAAGAGTAAAAGCAGGAACTGTGCGCATGGC TTCCTAGATTCAAGTTAAGGTCCGACTTTTGGTAGCCATCGGATTCGAGAAGTATCCCGCTCCAGGCGCT AATCCGCGTCCTGGCTGGTGACAGGGATCACCCGGAAGTTGAAACCTTTGACTTCCGGGTCACGATTAGT ACTGCCCCCCCCAAGGGCGGATCTTGAGCGCTTATTGGCTGTCATGCACATGGCCTCGCAGTGATTCGGC GGCTTGGAAGGAAGTGGCTCCGCCCATCCGCGCGAGTCCCAAGACTGGCTGGGCCATGGCGGTCCAGGCT CTGCGAGAGGAGTTGGACTCCAAGTGCCTACAGCTGCTCAGTGACCTGGAGGAACTGGAGGCGAAGCGGG CGGCGCTGAACGCCAGGGTGGAGGAGGGTTGGCTCTCACTTGCCAAGGCTCGCTATGCCATGGGAGCCAA GTCGGTAGGACCCCTGCAGTATGCCTCGCGTATGGAGCCTCAGGTCTGCGTGCGTGCCAGCGAGGCCCAG GATGGACCCCAGACCTTCAGGGTGATCAAAGCTGACGCCCAGACCCCCGAGGAAGTGGGGCCTAGCGAAG CATCTCTGCGCAGGCGCAAGGGCCCCACCAAGACCAAAGAGTTAGGATCTGCTGTAGTTCCTCAAGATCC CTTGAACTGGTTTGGAATTTTGGTTCCTCACAGTCTGCGGCAGGCCCAAGCCAGCTTCCGGGATGGCCTA CAGCTGGCTGCAGATATAGCCAGTCTCCAGACTCGCATCAACTGGGGTCAGAGTCAGCTTCGAGGCCTCC AGAAAAAACTGAAGGAGTTGGACCCTGGGCCTGCCTGACCTGCATCCACATTGGGAGGCACTGAGCATAG CTGCCGTGGATGCTACCACCTTACCTCAGGCCTTCTTCTTTTTATAAGCAGCCCCTGTCTCAGCTGATGC CCCACCTATAAATGTCCTTAGTCTGCGCGGTTTCTAGCTTTTCGAGGCATGAAGTTGAGTTATGAAGTTT GATGAAGCAAAGCTGAGCAGTGTAGAGAGCACCTAGTTCTAGGCCATGGTCTCAGGGCATGACAGTGACG TGTGAATAACCTGCGGCAGCCAGCTGGGATAGTGAAGCCCTGCAGAAATGGTGACAGAGAAGAAAGGGAG TCTAGAAACATAAGGTAGATCAGGACAATTCCAGTCAATAAAAGTACCTCAGGCTGGGTGTGGCAGCGCG TGCCTGTAGCTCCAGCAACTCTAGAGGCTGAGGCAGGAGGATCACTTAAGCCCTGGGAAGTTTATAAACA AGACAGCACACACACACACACACACAAATACCAGAATAGCTAGCAGGAAATATTACTTCTGTTATAAAAG GAAAAATAACTGTCATTCTTCCGGGGAATCAGGGTGACCTTCCTGATTGCACTTAGCTTATAGAGTGAGA CCCAGCTGCTTTGCTGAAGGTGCATTCTCTCACCTGGAAACTGTGTCTTGCTTCACGTTTTTTTCCTGAG CTATCCAATTGTGTTGGGAAGGGGAATGTAATTGTTTGTGGGGTGTAAATATTTCTTCCTAAGTTGTTAA TTTTGCTTATATTAGTTTTTCTACTTATGAATTGTAAAAACAGAAATTTGTACAGAAAAATTGAATAAAG TAAAAGCTACAGTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_027159
• Insert Size: 543 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019430, AAH19430
• RefSeq Size: 1709 bp
• RefSeq ORF: 543 bp
• Locus ID: 69668
• UniProt ID: Q8VE99
• Cytogenetics: 1 B
• Gene Summary: Accessory component of the proton-transporting vacuolar (V)-ATPase protein pump involved in intracellular iron homeostasis. In aerobic conditions, required for intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. Necessary for endolysosomal acidification and lysosomal degradation (By similarity). May be involved in Golgi homeostasis (By similarity).[UniProtKB/Swiss-Prot Function]