Litaf (NM_019980) Mouse Tagged ORF Clone
CAT#: MG201259
- TrueORF®
Litaf (tGFP-tagged) - Mouse LPS-induced TN factor (Litaf)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_019980" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Litaf |
Synonyms | 3222402J11Rik; C85531; N4WBP3; TBX1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201259 representing NM_019980
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCTCCAGGACCTTACCAAGCAGCTGCAGGCCCTTCAGTAGTGCCCACCGCACCCCCAACCTATG AAGAAACAGTGGGTGTCAACAGTTACTACCCAACGCCCCCAGCACCTATGCCGGGACCAGCCACAGGGCT CATTACAGGCCCAGATGGGAAGGGAATGAATCCACCTTCGTACTACACCCAGCCTGTGCCTGTCCCCAAC GCCAACGCAATTGCCGTGCAGACCGTTTATGTGCAGCAGCCTGTCTCCTTCTATGACCGCCCCGTCCAGA TGTGCTGTCCTTCCTGCAGCAAGATGATCGTGACCCAGCTGTCCTACAATGCGGGAGCCCTCACCTGGCT CTCCTGTGGCAGTCTGTGTCTGCTGGGATGCGTTGCTGGCTGCTGCTTCATCCCGTTCTGCGTAGACGCC CTACAGGATGTGGACCACTACTGCCCCAACTGCAAAGCGCTCCTGGGCACCTACAAGCGCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201259 representing NM_019980
Red=Cloning site Green=Tags(s) MSAPGPYQAAAGPSVVPTAPPTYEETVGVNSYYPTPPAPMPGPATGLITGPDGKGMNPPSYYTQPVPVPN ANAIAVQTVYVQQPVSFYDRPVQMCCPSCSKMIVTQLSYNAGALTWLSCGSLCLLGCVAGCCFIPFCVDA LQDVDHYCPNCKALLGTYKRL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019980 |
ORF Size | 483 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019980.2, NP_064364.1 |
RefSeq Size | 2205 bp |
RefSeq ORF | 486 bp |
Locus ID | 56722 |
UniProt ID | Q9JLJ0 |
Cytogenetics | 16 A1 |
Gene Summary | Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps downregulate downstream signaling cascades (PubMed:23166352). Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes. Probably plays a role in regulating protein degradation via its interaction with NEDD4 (By similarity). May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role with STAT6 in the nucleus in regulating the expression of various cytokines (PubMed:15793005, PubMed:21980379). May regulate the expression of numerous cytokines, such as TNF, CCL2, CCL5, CXCL1, IL1A and IL10 (PubMed:12355436, PubMed:15025820, PubMed:16954198, PubMed:21980379, PubMed:22160695).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207534 | Litaf (untagged) - Mouse LPS-induced TN factor (Litaf), (10ug) |
USD 165.00 |
|
MR201259 | Litaf (Myc-DDK-tagged) - Mouse LPS-induced TN factor (Litaf) |
USD 150.00 |
|
MR201259L3 | Lenti ORF clone of Litaf (Myc-DDK-tagged) - Mouse LPS-induced TN factor (Litaf) |
USD 450.00 |
|
MR201259L4 | Lenti ORF clone of Litaf (mGFP-tagged) - Mouse LPS-induced TN factor (Litaf) |
USD 450.00 |
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