Ampd3 (NM_001276301) Mouse Untagged Clone

CAT#: MC228889

Ampd3 (untagged) - Mouse adenosine monophosphate deaminase 3 (Ampd3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ampd3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228889 representing NM_001276301
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCGCCAGTTCCCCAAGTTGAACATGTCCGATTTGGACGAACATGTCCGGCTGTTGGCGGAGAAGG
  TGTTTGCTAAGGTGCTCCGAGAAGAGGACAGCAAAGATGTCATGTCCCTGTTTACTGTCCCCGAGGACTG
  TCCCATCGGACAGAAGGAAGCCAAGGAGAGGGAGCTACAGAAGGAGCTTGCAGAGCAGAAGTCTGTGGAG
  ACAGCAAAAAGAAAGAAGAGTTTCAAGATGATCCGGTCGCAGTCTTTGTCCCTGCAGATGCCAACACAAC
  AAGACTGGAAAGGTCCTCCAACTGCCAGTCCTGCCATGTCTCCTGCAACCCCTCTGGTTCCCGGAGCTAC
  TTCTAAGCCTGGGCCAGCACCCTATGCTATGCCTGAGTATCAGCGGGTCACCATCAGTGGAGATTACTGT
  GCAGGGATCACTGTGGAAGACTATGAGCAGGCTGCCAAGAGCCTGGCCAAGGCCCTGATGATCCGGGAGA
  AGTATGCACGGCTTGCCTACCACCGCTTCCCACGGACCACAGCCCAGTACCTGGCTCATCAGGGGGAAAG
  TGTGCCTTTGGAAGAGGGTCTCCCAGACTTCCACCCTCCCCCACTGCCCCAGGAAGACCCTTATTGCCTG
  GATGATGCACCCCCCAACCTGGGCTACCTGGTTCGCATGCACGGAGGTGTTCTCTTTGTGTATGACAATC
  AGACGATGTTGGAACGCCAGGAGCCCCACAGCCTGCCCTACCCTGACCTGGAGACCTACATTGTGGACAT
  GAGCCATATTCTGGCACTCATCACTGATGGCCCCACGAAAACCTATTGTCACCGGCGACTGAACTTTCTG
  GAATCCAAGTTTAGCCTTCATGAGATGTTAAATGAAATGTCAGAGTTCAAGGAATTGAAGAGTAACCCCC
  ACAGAGACTTTTATAATGTGAGAAAGGTAGACACACACATTCATGCAGCGGCCTGCATGAATCAGAAGCA
  CCTGCTGCGTTTCATCAAGCACACGTACCAGACAGAGCCCGACAGAACTGTGGCGGAGAAGCTAGGTCGG
  AAGATCACCCTGCGGCAGGTGTTTGACAGCCTGCACATGGACCCCTACGACCTCACGGTGGACTCGCTGG
  ATGTTCATGCAGGCCGCCAGACATTTCACCGCTTCGACAAGTTCAACTCCAAGTACAACCCCGTGGGGGC
  CAGCGAGCTCCGGGACCTGTATCTGAAAACTGAGAACTACCTCGGGGGAGAATACTTTGCTCGCATGGTC
  AAGGAAGTGGCCCGGGAGCTAGAGGACAGCAAGTACCAATACTCAGAACCCCGGCTCTCCATCTATGGTC
  GCAGTCCCAAGGAGTGGTCTAGCCTGGCCCGCTGGTTCATCCAGCACAAGGTCTACTCACCCAACATGCG
  CTGGATCATCCAGGTGCCCCGCATCTATGATATATTTAGGTCAAAGAAACTACTGCCAAACTTTGGGAAG
  ATGCTGGAGAACATCTTCCTGCCCCTGTTCAAAGCTACCATCAACCCCCAAGATCATCGGGAGCTTCACC
  TCTTCCTTAAATACGTGACTGGGTTTGACAGTGTGGATGACGAATCCAAGCACAGTGACCACATGTTCTC
  AGACAAGAGTCCCAGCCCAGACCTCTGGACCAGCGAGCAGAACCCGCCCTATAGTTACTACCTGTACTAC
  ATGTATGCCAACATCATGGTGCTGAACAACCTCCGCAGGGAACGAGGCCTGAGCACGTTCCTCTTCCGGC
  CTCACTGTGGGGAGGCCGGCTCCATCACTCACCTAGTGTCTGCCTTCCTGACGGCAGACAACATTTCCCA
  CGGGCTGCTGCTTAAGAAGAGCCCTGTTTTGCAGTACCTCTACTACCTTGCTCAGATCCCTATTGCCATG
  TCTCCTCTCAGCAACAACAGCCTGTTTCTGGAATATTCCAAGAACCCCCTCCGGGAATTCCTGCACAAGG
  GACTACATGTCTCTCTCTCTACCGATGACCCCATGCAGTTTCACTACACAAAGGAAGCGCTCATGGAGGA
  GTACGCCATTGCGGCACAAGTGTGGAAGCTGAGTACCTGCGACCTGTGTGAGATCGCCAGGAACAGTGTG
  CTGCAGAGTGGCCTCTCACATCAGGAGAAGCAGAAGTTTCTGGGTCAGAATTATTACAAGGAAGGACCAG
  AGGGCAATGACATTCGAAAGACAAATGTTGCACAGATCCGGATGGCATTCCGCTACGAGACCCTGTGTAA
  TGAGCTCAGCTTTCTGTCCGATGCCATGAAGTCCGAGGAGATCACAGCCCTGACCAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276301
• Insert Size: 2301 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276301.1, NP_001263230.1
• RefSeq Size: 3979 bp
• RefSeq ORF: 2301 bp
• Locus ID: 11717
• UniProt ID: O08739
• Cytogenetics: 7 57.85 cM
• Gene Summary: This gene encodes a member of the adenosine and AMP deaminases family. The encoded protein is an AMP deaminase involved in nucleotide and energy metabolism in erythrocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (2) uses an alternate 5' exon, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.